Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome
Cytokinins are multifaceted plant hormones that play crucial roles in plant interactions with the environment. Modulations in cytokinin metabolism and signaling have been successfully used for elevating plant tolerance to biotic and abiotic stressors. Here, we analyzed <i>Arabidopsis thaliana&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/11/1563 |
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| author | Veronika Berková Michaela Kameniarová Vladěna Ondrisková Miroslav Berka Simona Menšíková Romana Kopecká Markéta Luklová Jan Novák Lukáš Spíchal Aaron M. Rashotte Břetislav Brzobohatý Martin Černý |
| author_facet | Veronika Berková Michaela Kameniarová Vladěna Ondrisková Miroslav Berka Simona Menšíková Romana Kopecká Markéta Luklová Jan Novák Lukáš Spíchal Aaron M. Rashotte Břetislav Brzobohatý Martin Černý |
| author_sort | Veronika Berková |
| collection | DOAJ |
| description | Cytokinins are multifaceted plant hormones that play crucial roles in plant interactions with the environment. Modulations in cytokinin metabolism and signaling have been successfully used for elevating plant tolerance to biotic and abiotic stressors. Here, we analyzed <i>Arabidopsis thaliana</i> response to INhibitor of CYtokinin DEgradation (INCYDE), a potent inhibitor of cytokinin dehydrogenase. We found that at low nanomolar concentration, the effect of INCYCE on seedling growth and development was not significantly different from that of trans-Zeatin treatment. However, an alteration in the spatial distribution of cytokinin signaling was found at low micromolar concentrations, and proteomics analysis revealed a significant impact on the molecular level. An in-depth proteome analysis of an early (24 h) response and a dose-dependent response after 168 h highlighted the effects on primary and secondary metabolism, including alterations in ribosomal subunits, RNA metabolism, modulations of proteins associated with chromatin, and the flavonoid and phenylpropanoid biosynthetic pathway. The observed attenuation in stress-response mechanisms, including abscisic acid signaling and the metabolism of jasmonates, could explain previously reported positive effects of INCYDE under mild stress conditions. |
| first_indexed | 2024-03-10T14:52:34Z |
| format | Article |
| id | doaj.art-1a797899274d4666b12f4a5b96f36942 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T14:52:34Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-1a797899274d4666b12f4a5b96f369422023-11-20T20:49:27ZengMDPI AGPlants2223-77472020-11-01911156310.3390/plants9111563Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant ProteomeVeronika Berková0Michaela Kameniarová1Vladěna Ondrisková2Miroslav Berka3Simona Menšíková4Romana Kopecká5Markéta Luklová6Jan Novák7Lukáš Spíchal8Aaron M. Rashotte9Břetislav Brzobohatý10Martin Černý11Department of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicDepartment of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicDepartment of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicDepartment of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicDepartment of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicDepartment of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicDepartment of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicDepartment of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicDepartment of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, 77200 Olomouc, Czech RepublicDepartment of Biological Sciences, Auburn University, Auburn, AL 811, USADepartment of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicDepartment of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech RepublicCytokinins are multifaceted plant hormones that play crucial roles in plant interactions with the environment. Modulations in cytokinin metabolism and signaling have been successfully used for elevating plant tolerance to biotic and abiotic stressors. Here, we analyzed <i>Arabidopsis thaliana</i> response to INhibitor of CYtokinin DEgradation (INCYDE), a potent inhibitor of cytokinin dehydrogenase. We found that at low nanomolar concentration, the effect of INCYCE on seedling growth and development was not significantly different from that of trans-Zeatin treatment. However, an alteration in the spatial distribution of cytokinin signaling was found at low micromolar concentrations, and proteomics analysis revealed a significant impact on the molecular level. An in-depth proteome analysis of an early (24 h) response and a dose-dependent response after 168 h highlighted the effects on primary and secondary metabolism, including alterations in ribosomal subunits, RNA metabolism, modulations of proteins associated with chromatin, and the flavonoid and phenylpropanoid biosynthetic pathway. The observed attenuation in stress-response mechanisms, including abscisic acid signaling and the metabolism of jasmonates, could explain previously reported positive effects of INCYDE under mild stress conditions.https://www.mdpi.com/2223-7747/9/11/1563cytokininCKXinhibitor of cytokinin degradationproteomeArabidopsis thalianastress response attenuation |
| spellingShingle | Veronika Berková Michaela Kameniarová Vladěna Ondrisková Miroslav Berka Simona Menšíková Romana Kopecká Markéta Luklová Jan Novák Lukáš Spíchal Aaron M. Rashotte Břetislav Brzobohatý Martin Černý Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome Plants cytokinin CKX inhibitor of cytokinin degradation proteome Arabidopsis thaliana stress response attenuation |
| title | Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome |
| title_full | Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome |
| title_fullStr | Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome |
| title_full_unstemmed | Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome |
| title_short | Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome |
| title_sort | arabidopsis response to inhibitor of cytokinin degradation incyde modulations of cytokinin signaling and plant proteome |
| topic | cytokinin CKX inhibitor of cytokinin degradation proteome Arabidopsis thaliana stress response attenuation |
| url | https://www.mdpi.com/2223-7747/9/11/1563 |
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