Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand Approaches
A novel approach, in the context of bioprinting, is the targeted printing of a defined number of cells at desired positions in predefined locations, which thereby opens up new perspectives for life science engineering. One major challenge in this application is to realize the targeted printing of ce...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/8/4/206 |
| _version_ | 1827628743158398976 |
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| author | Emine Karakaya Faina Bider Andreas Frank Jörg Teßmar Lisa Schöbel Leonard Forster Stefan Schrüfer Hans-Werner Schmidt Dirk Wolfram Schubert Andreas Blaeser Aldo R. Boccaccini Rainer Detsch |
| author_facet | Emine Karakaya Faina Bider Andreas Frank Jörg Teßmar Lisa Schöbel Leonard Forster Stefan Schrüfer Hans-Werner Schmidt Dirk Wolfram Schubert Andreas Blaeser Aldo R. Boccaccini Rainer Detsch |
| author_sort | Emine Karakaya |
| collection | DOAJ |
| description | A novel approach, in the context of bioprinting, is the targeted printing of a defined number of cells at desired positions in predefined locations, which thereby opens up new perspectives for life science engineering. One major challenge in this application is to realize the targeted printing of cells onto a gel substrate with high cell survival rates in advanced bioinks. For this purpose, different alginate-dialdehyde—polyethylene glycol (ADA-PEG) inks with different PEG modifications and chain lengths (1–8 kDa) were characterized to evaluate their application as bioinks for drop on demand (DoD) printing. The biochemical properties of the inks, printing process, NIH/3T3 fibroblast cell distribution within a droplet and shear forces during printing were analyzed. Finally, different hydrogels were evaluated as a printing substrate. By analysing different PEG chain lengths with covalently crosslinked and non-crosslinked ADA-PEG inks, it was shown that the influence of Schiff’s bases on the viscosity of the corresponding materials is very low. Furthermore, it was shown that longer polymer chains resulted in less stable hydrogels, leading to fast degradation rates. Several bioinks highly exhibit biocompatibility, while the calculated nozzle shear stress increased from approx. 1.3 and 2.3 kPa. Moreover, we determined the number of cells for printed droplets depending on the initial cell concentration, which is crucially needed for targeted cell printing approaches. |
| first_indexed | 2024-03-09T13:39:03Z |
| format | Article |
| id | doaj.art-1a7c856f4cb343339a6cbdee91c73cf3 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T13:39:03Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-1a7c856f4cb343339a6cbdee91c73cf32023-11-30T21:09:02ZengMDPI AGGels2310-28612022-03-018420610.3390/gels8040206Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand ApproachesEmine Karakaya0Faina Bider1Andreas Frank2Jörg Teßmar3Lisa Schöbel4Leonard Forster5Stefan Schrüfer6Hans-Werner Schmidt7Dirk Wolfram Schubert8Andreas Blaeser9Aldo R. Boccaccini10Rainer Detsch11Department of Materials Science and Engineering, Institute of Biomaterials, Friedrich-Alexander University Erlangen-Nürnberg, Cauerstraße 6, 91058 Erlangen, GermanyDepartment of Materials Science and Engineering, Institute of Biomaterials, Friedrich-Alexander University Erlangen-Nürnberg, Cauerstraße 6, 91058 Erlangen, GermanyMacromolecular Chemistry I and Bavarian Polymer Institute (BPI), University of Bayreuth, Universitätsstrasse 30, 95447 Bayreuth, GermanyDepartment of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute (BPI), University of Würzburg, Pleicherwall 2, 97070 Würzburg, GermanyDepartment of Materials Science and Engineering, Institute of Biomaterials, Friedrich-Alexander University Erlangen-Nürnberg, Cauerstraße 6, 91058 Erlangen, GermanyDepartment of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute (BPI), University of Würzburg, Pleicherwall 2, 97070 Würzburg, GermanyDepartment of Materials Science and Engineering, Institute of Polymer Materials, University Erlangen-Nürnberg, Martenstraße 7, 91058 Erlangen, GermanyMacromolecular Chemistry I and Bavarian Polymer Institute (BPI), University of Bayreuth, Universitätsstrasse 30, 95447 Bayreuth, GermanyDepartment of Materials Science and Engineering, Institute of Polymer Materials, University Erlangen-Nürnberg, Martenstraße 7, 91058 Erlangen, GermanyDepartment of Mechanical Engineering, BioMedical Printing Technology, Technical University of Darmstadt, Magdalenenstr. 2, 64289 Darmstadt, GermanyDepartment of Materials Science and Engineering, Institute of Biomaterials, Friedrich-Alexander University Erlangen-Nürnberg, Cauerstraße 6, 91058 Erlangen, GermanyDepartment of Materials Science and Engineering, Institute of Biomaterials, Friedrich-Alexander University Erlangen-Nürnberg, Cauerstraße 6, 91058 Erlangen, GermanyA novel approach, in the context of bioprinting, is the targeted printing of a defined number of cells at desired positions in predefined locations, which thereby opens up new perspectives for life science engineering. One major challenge in this application is to realize the targeted printing of cells onto a gel substrate with high cell survival rates in advanced bioinks. For this purpose, different alginate-dialdehyde—polyethylene glycol (ADA-PEG) inks with different PEG modifications and chain lengths (1–8 kDa) were characterized to evaluate their application as bioinks for drop on demand (DoD) printing. The biochemical properties of the inks, printing process, NIH/3T3 fibroblast cell distribution within a droplet and shear forces during printing were analyzed. Finally, different hydrogels were evaluated as a printing substrate. By analysing different PEG chain lengths with covalently crosslinked and non-crosslinked ADA-PEG inks, it was shown that the influence of Schiff’s bases on the viscosity of the corresponding materials is very low. Furthermore, it was shown that longer polymer chains resulted in less stable hydrogels, leading to fast degradation rates. Several bioinks highly exhibit biocompatibility, while the calculated nozzle shear stress increased from approx. 1.3 and 2.3 kPa. Moreover, we determined the number of cells for printed droplets depending on the initial cell concentration, which is crucially needed for targeted cell printing approaches.https://www.mdpi.com/2310-2861/8/4/206bioprintingdrop on demandsodium alginatepolyethylene glycolshear stress |
| spellingShingle | Emine Karakaya Faina Bider Andreas Frank Jörg Teßmar Lisa Schöbel Leonard Forster Stefan Schrüfer Hans-Werner Schmidt Dirk Wolfram Schubert Andreas Blaeser Aldo R. Boccaccini Rainer Detsch Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand Approaches Gels bioprinting drop on demand sodium alginate polyethylene glycol shear stress |
| title | Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand Approaches |
| title_full | Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand Approaches |
| title_fullStr | Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand Approaches |
| title_full_unstemmed | Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand Approaches |
| title_short | Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand Approaches |
| title_sort | targeted printing of cells evaluation of ada peg bioinks for drop on demand approaches |
| topic | bioprinting drop on demand sodium alginate polyethylene glycol shear stress |
| url | https://www.mdpi.com/2310-2861/8/4/206 |
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