Gut mycobiome dysbiosis in rats showing retinal changes indicative of diabetic retinopathy.
The current study compared the gut mycobiomes of diabetic rats generated by a streptozotocin chemical challenge, diabetic rats with retinal changes and normal control rats over a period of 4 months. Sustained increase in blood sugar levels (>150 mg/dL) confirmed the induction of diabetes. Histolo...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0267080 |
| _version_ | 1828723949979041792 |
|---|---|
| author | Shalem Raj Padakandla Taraprasad Das Gumpili Sai Prashanthi Kiran Kumar Angadi S Sreenivasa Reddy G Bhanuprakash Reddy Sisinthy Shivaji |
| author_facet | Shalem Raj Padakandla Taraprasad Das Gumpili Sai Prashanthi Kiran Kumar Angadi S Sreenivasa Reddy G Bhanuprakash Reddy Sisinthy Shivaji |
| author_sort | Shalem Raj Padakandla |
| collection | DOAJ |
| description | The current study compared the gut mycobiomes of diabetic rats generated by a streptozotocin chemical challenge, diabetic rats with retinal changes and normal control rats over a period of 4 months. Sustained increase in blood sugar levels (>150 mg/dL) confirmed the induction of diabetes. Histology and immunohistochemistry were used to identify changes in the retinal tissues in the diabetic rats indicative of the animals progressing into diabetic retinopathy. Gut mycobiomes generated using faecal DNA, indicated dysbiosis at the genus level in both diabetic (DM) and diabetic rats with retinal changes (DRC) when compared with the control rats. In Tables 3-6 the specific genera that were significantly increased/decreased in DM1 and DM2 and in DRC1 and DRC2 respectively compared to the respective controls CT1-CT4 rats are listed. Further, the mycobiomes of the DM and DRC rats separated into distinct clusters following heat-map analysis of the discriminating genera. In addition, β-diversity analysis separated the mycobiomes of DM and DRC rats from that of the control rats, but the mycobiomes of diabetic rats and diabetic rats with retinal changes showed an overlap. Based on the inferred functions of the discriminating genera in the mycobiomes, we speculated that increase in pathogenic fungi might contribute to the inflammatory status both in diabetic rats and rats showing retinal changes. |
| first_indexed | 2024-04-12T12:59:49Z |
| format | Article |
| id | doaj.art-1a7ef14e77f749d98a9cd8957b4e9bf3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T12:59:49Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1a7ef14e77f749d98a9cd8957b4e9bf32022-12-22T03:32:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01174e026708010.1371/journal.pone.0267080Gut mycobiome dysbiosis in rats showing retinal changes indicative of diabetic retinopathy.Shalem Raj PadakandlaTaraprasad DasGumpili Sai PrashanthiKiran Kumar AngadiS Sreenivasa ReddyG Bhanuprakash ReddySisinthy ShivajiThe current study compared the gut mycobiomes of diabetic rats generated by a streptozotocin chemical challenge, diabetic rats with retinal changes and normal control rats over a period of 4 months. Sustained increase in blood sugar levels (>150 mg/dL) confirmed the induction of diabetes. Histology and immunohistochemistry were used to identify changes in the retinal tissues in the diabetic rats indicative of the animals progressing into diabetic retinopathy. Gut mycobiomes generated using faecal DNA, indicated dysbiosis at the genus level in both diabetic (DM) and diabetic rats with retinal changes (DRC) when compared with the control rats. In Tables 3-6 the specific genera that were significantly increased/decreased in DM1 and DM2 and in DRC1 and DRC2 respectively compared to the respective controls CT1-CT4 rats are listed. Further, the mycobiomes of the DM and DRC rats separated into distinct clusters following heat-map analysis of the discriminating genera. In addition, β-diversity analysis separated the mycobiomes of DM and DRC rats from that of the control rats, but the mycobiomes of diabetic rats and diabetic rats with retinal changes showed an overlap. Based on the inferred functions of the discriminating genera in the mycobiomes, we speculated that increase in pathogenic fungi might contribute to the inflammatory status both in diabetic rats and rats showing retinal changes.https://doi.org/10.1371/journal.pone.0267080 |
| spellingShingle | Shalem Raj Padakandla Taraprasad Das Gumpili Sai Prashanthi Kiran Kumar Angadi S Sreenivasa Reddy G Bhanuprakash Reddy Sisinthy Shivaji Gut mycobiome dysbiosis in rats showing retinal changes indicative of diabetic retinopathy. PLoS ONE |
| title | Gut mycobiome dysbiosis in rats showing retinal changes indicative of diabetic retinopathy. |
| title_full | Gut mycobiome dysbiosis in rats showing retinal changes indicative of diabetic retinopathy. |
| title_fullStr | Gut mycobiome dysbiosis in rats showing retinal changes indicative of diabetic retinopathy. |
| title_full_unstemmed | Gut mycobiome dysbiosis in rats showing retinal changes indicative of diabetic retinopathy. |
| title_short | Gut mycobiome dysbiosis in rats showing retinal changes indicative of diabetic retinopathy. |
| title_sort | gut mycobiome dysbiosis in rats showing retinal changes indicative of diabetic retinopathy |
| url | https://doi.org/10.1371/journal.pone.0267080 |
| work_keys_str_mv | AT shalemrajpadakandla gutmycobiomedysbiosisinratsshowingretinalchangesindicativeofdiabeticretinopathy AT taraprasaddas gutmycobiomedysbiosisinratsshowingretinalchangesindicativeofdiabeticretinopathy AT gumpilisaiprashanthi gutmycobiomedysbiosisinratsshowingretinalchangesindicativeofdiabeticretinopathy AT kirankumarangadi gutmycobiomedysbiosisinratsshowingretinalchangesindicativeofdiabeticretinopathy AT ssreenivasareddy gutmycobiomedysbiosisinratsshowingretinalchangesindicativeofdiabeticretinopathy AT gbhanuprakashreddy gutmycobiomedysbiosisinratsshowingretinalchangesindicativeofdiabeticretinopathy AT sisinthyshivaji gutmycobiomedysbiosisinratsshowingretinalchangesindicativeofdiabeticretinopathy |