Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing
Green tea drinking can ameliorate postmenopausal osteoporosis by increasing the bone mineral density. (-)-Epigallocatechin-3-gallate (EGCG), the abundant and active compound of tea catechin, was proven to be able to reduce bone loss and ameliorate microarchitecture in female ovariectomized rats. EGC...
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MDPI AG
2020-04-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/4/620 |
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| author | Sung-Yen Lin Jung Yu Kan Cheng-Chang Lu Han Hsiang Huang Tsung-Lin Cheng Hsuan-Ti Huang Cheng-Jung Ho Tien-Ching Lee Shu-Chun Chuang Yi-Shan Lin Lin Kang Chung-Hwan Chen |
| author_facet | Sung-Yen Lin Jung Yu Kan Cheng-Chang Lu Han Hsiang Huang Tsung-Lin Cheng Hsuan-Ti Huang Cheng-Jung Ho Tien-Ching Lee Shu-Chun Chuang Yi-Shan Lin Lin Kang Chung-Hwan Chen |
| author_sort | Sung-Yen Lin |
| collection | DOAJ |
| description | Green tea drinking can ameliorate postmenopausal osteoporosis by increasing the bone mineral density. (-)-Epigallocatechin-3-gallate (EGCG), the abundant and active compound of tea catechin, was proven to be able to reduce bone loss and ameliorate microarchitecture in female ovariectomized rats. EGCG can also enhance the osteogenic differentiation of murine bone marrow mesenchymal stem cells and inhibit the osteoclastogenesis in RAW264.7 cells by modulation of the receptor activator of nuclear factor-kB (RANK)/RANK ligand (RANKL)/osteoprotegrin (OPG) (RANK/RANKL/OPG) pathway. Our previous study also found that EGCG can promote bone defect healing in the distal femur partially via bone morphogenetic protein-2 (BMP-2). Considering the osteoinduction property of BMP-2, we hypothesized that EGCG could accelerate the bone healing process with an increased expression of BMP-2. In this manuscript, we studied whether the local use of EGCG can facilitate tibial fracture healing. Fifty-six 4-month-old rats were randomly assigned to two groups after being weight-matched: a control group with vehicle treatment (Ctrl) and a study group with 10 µmol/L, 40 µL, EGCG treatment (EGCG). Two days after the operation, the rats were treated daily with EGCG or vehicle by percutaneous local injection for 2 weeks. The application of EGCG enhanced callus formation by increasing the bone volume and subsequently improved the mechanical properties of the tibial bone, including the maximal load, break load, stiffness, and Young’s modulus. The results of the histology and BMP-2 immunohistochemistry staining showed that EGCG treatment accelerated the bone matrix formation and produced a stronger expression of BMP-2. Taken together, this study for the first time demonstrated that local treatment of EGCG can accelerate the fracture healing process at least partly via BMP-2. |
| first_indexed | 2024-03-10T20:24:50Z |
| format | Article |
| id | doaj.art-1a80ceb9aeff4e8480887ff96ff39931 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T20:24:50Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-1a80ceb9aeff4e8480887ff96ff399312023-11-19T21:49:54ZengMDPI AGBiomolecules2218-273X2020-04-0110462010.3390/biom10040620Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture HealingSung-Yen Lin0Jung Yu Kan1Cheng-Chang Lu2Han Hsiang Huang3Tsung-Lin Cheng4Hsuan-Ti Huang5Cheng-Jung Ho6Tien-Ching Lee7Shu-Chun Chuang8Yi-Shan Lin9Lin Kang10Chung-Hwan Chen11Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80708, TaiwanGraduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80708, TaiwanDepartment of Veterinary Medicine, National Chiayi University, Chiayi City 60054, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80708, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80708, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80708, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80708, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80708, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80708, TaiwanDepartment of Obstetrics and Gynecology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70101, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80708, TaiwanGreen tea drinking can ameliorate postmenopausal osteoporosis by increasing the bone mineral density. (-)-Epigallocatechin-3-gallate (EGCG), the abundant and active compound of tea catechin, was proven to be able to reduce bone loss and ameliorate microarchitecture in female ovariectomized rats. EGCG can also enhance the osteogenic differentiation of murine bone marrow mesenchymal stem cells and inhibit the osteoclastogenesis in RAW264.7 cells by modulation of the receptor activator of nuclear factor-kB (RANK)/RANK ligand (RANKL)/osteoprotegrin (OPG) (RANK/RANKL/OPG) pathway. Our previous study also found that EGCG can promote bone defect healing in the distal femur partially via bone morphogenetic protein-2 (BMP-2). Considering the osteoinduction property of BMP-2, we hypothesized that EGCG could accelerate the bone healing process with an increased expression of BMP-2. In this manuscript, we studied whether the local use of EGCG can facilitate tibial fracture healing. Fifty-six 4-month-old rats were randomly assigned to two groups after being weight-matched: a control group with vehicle treatment (Ctrl) and a study group with 10 µmol/L, 40 µL, EGCG treatment (EGCG). Two days after the operation, the rats were treated daily with EGCG or vehicle by percutaneous local injection for 2 weeks. The application of EGCG enhanced callus formation by increasing the bone volume and subsequently improved the mechanical properties of the tibial bone, including the maximal load, break load, stiffness, and Young’s modulus. The results of the histology and BMP-2 immunohistochemistry staining showed that EGCG treatment accelerated the bone matrix formation and produced a stronger expression of BMP-2. Taken together, this study for the first time demonstrated that local treatment of EGCG can accelerate the fracture healing process at least partly via BMP-2.https://www.mdpi.com/2218-273X/10/4/620(-)-epigallocatechin-3-gallate (EGCG)bone morphogenetic protein-2 (BMP-2)catethinfracture healinglocal use |
| spellingShingle | Sung-Yen Lin Jung Yu Kan Cheng-Chang Lu Han Hsiang Huang Tsung-Lin Cheng Hsuan-Ti Huang Cheng-Jung Ho Tien-Ching Lee Shu-Chun Chuang Yi-Shan Lin Lin Kang Chung-Hwan Chen Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing Biomolecules (-)-epigallocatechin-3-gallate (EGCG) bone morphogenetic protein-2 (BMP-2) catethin fracture healing local use |
| title | Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing |
| title_full | Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing |
| title_fullStr | Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing |
| title_full_unstemmed | Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing |
| title_short | Green Tea Catechin (-)-Epigallocatechin-3-Gallate (EGCG) Facilitates Fracture Healing |
| title_sort | green tea catechin epigallocatechin 3 gallate egcg facilitates fracture healing |
| topic | (-)-epigallocatechin-3-gallate (EGCG) bone morphogenetic protein-2 (BMP-2) catethin fracture healing local use |
| url | https://www.mdpi.com/2218-273X/10/4/620 |
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