Evidence for an interaction between cannabinoidergic and dopaminergic systems with melanocortin MC3/ MC4 receptors in regulating food intake of neonatal chick
The current study aimed to see how the central dopaminergic and cannabinoidergic mechanisms affect melanocortin-induced food intake in the neonatal layer chickens. In this regard, 9 experiments were designed. In experiment 1, chicks injected with control solution, MTII (2.5, 5, and 10 ng). In experi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Ferdowsi University of Mashhad
2021-12-01
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| Series: | The Iranian Journal of Veterinary Science and Technology |
| Subjects: | |
| Online Access: | https://ijvst.um.ac.ir/article_40556_b74f4f3cd883aca75fabda1a45742302.pdf |
| _version_ | 1818083005736943616 |
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| author | Mohammad Bameri Morteza Zendedel kheybari Bita Vazir Ahmad Asghari Negar Panahi |
| author_facet | Mohammad Bameri Morteza Zendedel kheybari Bita Vazir Ahmad Asghari Negar Panahi |
| author_sort | Mohammad Bameri |
| collection | DOAJ |
| description | The current study aimed to see how the central dopaminergic and cannabinoidergic mechanisms affect melanocortin-induced food intake in the neonatal layer chickens. In this regard, 9 experiments were designed. In experiment 1, chicks injected with control solution, MTII (2.5, 5, and 10 ng). In experiment 2, control solution, L-DOPA (125 nmol), MTII (10 ng), and L-DOPA + MTII were applied to the birds. Experiments 3-9 were similar to experiment 2, except birds injected with 6-OHDA (150 nmol), SCH23390 (5 nmol), AMI-193 (5 nmol), NGB2904 (6.4 nmol), L-741,742 (6 nmol), SR141716A (6.25 µg), and AM630 (5 µg) instead of L-DOPA. Then, cumulative food intake was recorded at 30, 60, and 120 min following injection. According to the results, in comparison with the control group, dose-dependent hypophagia was observed in 3-h food-deprived neonatal layer chickens following ICV injection of MTII (2.5, 5, and 10 ng) (P<0.05). ICV injection of L-DOPA and SR141716A increased hypophagia induced by MTII in chickens (P<0.05), while 6-OHDA greatly suppressed MTII- induced hypophagia (P<0.05). In addition, SCH23390 and AMI-193 greatly weakened the MTII-induced hypophagia in neonatal layer chickens (P<0.05). However, NGB2904, L-741742 and AM630 had no role on hypophagia induced by MTII (P>0.05). These results demonstrated that melanocortin-induced hypophagia in the neonatal layer chickens is likely mediated by D1, D2, and CB1 receptors. |
| first_indexed | 2024-12-10T19:31:07Z |
| format | Article |
| id | doaj.art-1a817ca2c9f14fbab6d677d92ba64370 |
| institution | Directory Open Access Journal |
| issn | 2008-465X 2423-6306 |
| language | English |
| last_indexed | 2024-12-10T19:31:07Z |
| publishDate | 2021-12-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | The Iranian Journal of Veterinary Science and Technology |
| spelling | doaj.art-1a817ca2c9f14fbab6d677d92ba643702022-12-22T01:36:14ZengFerdowsi University of MashhadThe Iranian Journal of Veterinary Science and Technology2008-465X2423-63062021-12-01132374510.22067/ijvst.2021.69380.102840556Evidence for an interaction between cannabinoidergic and dopaminergic systems with melanocortin MC3/ MC4 receptors in regulating food intake of neonatal chickMohammad Bameri0Morteza Zendedel kheybari1Bita Vazir2Ahmad Asghari3Negar Panahi4Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of Clinical Science, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.The current study aimed to see how the central dopaminergic and cannabinoidergic mechanisms affect melanocortin-induced food intake in the neonatal layer chickens. In this regard, 9 experiments were designed. In experiment 1, chicks injected with control solution, MTII (2.5, 5, and 10 ng). In experiment 2, control solution, L-DOPA (125 nmol), MTII (10 ng), and L-DOPA + MTII were applied to the birds. Experiments 3-9 were similar to experiment 2, except birds injected with 6-OHDA (150 nmol), SCH23390 (5 nmol), AMI-193 (5 nmol), NGB2904 (6.4 nmol), L-741,742 (6 nmol), SR141716A (6.25 µg), and AM630 (5 µg) instead of L-DOPA. Then, cumulative food intake was recorded at 30, 60, and 120 min following injection. According to the results, in comparison with the control group, dose-dependent hypophagia was observed in 3-h food-deprived neonatal layer chickens following ICV injection of MTII (2.5, 5, and 10 ng) (P<0.05). ICV injection of L-DOPA and SR141716A increased hypophagia induced by MTII in chickens (P<0.05), while 6-OHDA greatly suppressed MTII- induced hypophagia (P<0.05). In addition, SCH23390 and AMI-193 greatly weakened the MTII-induced hypophagia in neonatal layer chickens (P<0.05). However, NGB2904, L-741742 and AM630 had no role on hypophagia induced by MTII (P>0.05). These results demonstrated that melanocortin-induced hypophagia in the neonatal layer chickens is likely mediated by D1, D2, and CB1 receptors.https://ijvst.um.ac.ir/article_40556_b74f4f3cd883aca75fabda1a45742302.pdfdopaminecannabinoidmelanocortinfood intakelayer chicken |
| spellingShingle | Mohammad Bameri Morteza Zendedel kheybari Bita Vazir Ahmad Asghari Negar Panahi Evidence for an interaction between cannabinoidergic and dopaminergic systems with melanocortin MC3/ MC4 receptors in regulating food intake of neonatal chick The Iranian Journal of Veterinary Science and Technology dopamine cannabinoid melanocortin food intake layer chicken |
| title | Evidence for an interaction between cannabinoidergic and dopaminergic systems with melanocortin MC3/ MC4 receptors in regulating food intake of neonatal chick |
| title_full | Evidence for an interaction between cannabinoidergic and dopaminergic systems with melanocortin MC3/ MC4 receptors in regulating food intake of neonatal chick |
| title_fullStr | Evidence for an interaction between cannabinoidergic and dopaminergic systems with melanocortin MC3/ MC4 receptors in regulating food intake of neonatal chick |
| title_full_unstemmed | Evidence for an interaction between cannabinoidergic and dopaminergic systems with melanocortin MC3/ MC4 receptors in regulating food intake of neonatal chick |
| title_short | Evidence for an interaction between cannabinoidergic and dopaminergic systems with melanocortin MC3/ MC4 receptors in regulating food intake of neonatal chick |
| title_sort | evidence for an interaction between cannabinoidergic and dopaminergic systems with melanocortin mc3 mc4 receptors in regulating food intake of neonatal chick |
| topic | dopamine cannabinoid melanocortin food intake layer chicken |
| url | https://ijvst.um.ac.ir/article_40556_b74f4f3cd883aca75fabda1a45742302.pdf |
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