Detection of insecticide resistance markers in Anopheles funestus from the Democratic Republic of the Congo using a targeted amplicon sequencing panel
Abstract Vector control strategies have been successful in reducing the number of malaria cases and deaths globally, but the spread of insecticide resistance represents a significant threat to disease control. Insecticide resistance has been reported across Anopheles (An.) vector populations, includ...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-44457-0 |
| _version_ | 1797560174070726656 |
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| author | Holly Acford-Palmer Monica Campos Janvier Bandibabone Sévérin N’Do Chimanuka Bantuzeko Bertin Zawadi Thomas Walker Jody E. Phelan Louisa A. Messenger Taane G. Clark Susana Campino |
| author_facet | Holly Acford-Palmer Monica Campos Janvier Bandibabone Sévérin N’Do Chimanuka Bantuzeko Bertin Zawadi Thomas Walker Jody E. Phelan Louisa A. Messenger Taane G. Clark Susana Campino |
| author_sort | Holly Acford-Palmer |
| collection | DOAJ |
| description | Abstract Vector control strategies have been successful in reducing the number of malaria cases and deaths globally, but the spread of insecticide resistance represents a significant threat to disease control. Insecticide resistance has been reported across Anopheles (An.) vector populations, including species within the An. funestus group. These mosquitoes are responsible for intense malaria transmission across sub-Saharan Africa, including in the Democratic Republic of the Congo (DRC), a country contributing > 12% of global malaria infections and mortality events. To support the continuous efficacy of vector control strategies, it is essential to monitor insecticide resistance using molecular surveillance tools. In this study, we developed an amplicon sequencing (“Amp-seq”) approach targeting An. funestus, and using multiplex PCR, dual index barcoding, and next-generation sequencing for high throughput and low-cost applications. Using our Amp-seq approach, we screened 80 An. funestus field isolates from the DRC across a panel of nine genes with mutations linked to insecticide resistance (ace-1, CYP6P4, CYP6P9a, GSTe2, vgsc, and rdl) and mosquito speciation (cox-1, mtND5, and ITS2). Amongst the 18 non-synonymous mutations detected, was N485I, in the ace-1 gene associated with carbamate resistance. Overall, our panel represents an extendable and much-needed method for the molecular surveillance of insecticide resistance in An. funestus populations. |
| first_indexed | 2024-03-10T17:56:41Z |
| format | Article |
| id | doaj.art-1a838087c29d4628853c4f8af7af7fd3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:56:41Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1a838087c29d4628853c4f8af7af7fd32023-11-20T09:10:16ZengNature PortfolioScientific Reports2045-23222023-10-0113111010.1038/s41598-023-44457-0Detection of insecticide resistance markers in Anopheles funestus from the Democratic Republic of the Congo using a targeted amplicon sequencing panelHolly Acford-Palmer0Monica Campos1Janvier Bandibabone2Sévérin N’Do3Chimanuka Bantuzeko4Bertin Zawadi5Thomas Walker6Jody E. Phelan7Louisa A. Messenger8Taane G. Clark9Susana Campino10Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical MedicineDepartment of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical MedicineCentre de Recherche en Sciences Naturelles de LwiroMédecins Sans Frontières (MSF) OCBACentre de Recherche en Sciences Naturelles de LwiroCentre de Recherche en Sciences Naturelles de LwiroSchool of Life Sciences, Gibbet Hill Campus, University of WarwickDepartment of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical MedicineDepartment of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical MedicineDepartment of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical MedicineDepartment of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical MedicineAbstract Vector control strategies have been successful in reducing the number of malaria cases and deaths globally, but the spread of insecticide resistance represents a significant threat to disease control. Insecticide resistance has been reported across Anopheles (An.) vector populations, including species within the An. funestus group. These mosquitoes are responsible for intense malaria transmission across sub-Saharan Africa, including in the Democratic Republic of the Congo (DRC), a country contributing > 12% of global malaria infections and mortality events. To support the continuous efficacy of vector control strategies, it is essential to monitor insecticide resistance using molecular surveillance tools. In this study, we developed an amplicon sequencing (“Amp-seq”) approach targeting An. funestus, and using multiplex PCR, dual index barcoding, and next-generation sequencing for high throughput and low-cost applications. Using our Amp-seq approach, we screened 80 An. funestus field isolates from the DRC across a panel of nine genes with mutations linked to insecticide resistance (ace-1, CYP6P4, CYP6P9a, GSTe2, vgsc, and rdl) and mosquito speciation (cox-1, mtND5, and ITS2). Amongst the 18 non-synonymous mutations detected, was N485I, in the ace-1 gene associated with carbamate resistance. Overall, our panel represents an extendable and much-needed method for the molecular surveillance of insecticide resistance in An. funestus populations.https://doi.org/10.1038/s41598-023-44457-0 |
| spellingShingle | Holly Acford-Palmer Monica Campos Janvier Bandibabone Sévérin N’Do Chimanuka Bantuzeko Bertin Zawadi Thomas Walker Jody E. Phelan Louisa A. Messenger Taane G. Clark Susana Campino Detection of insecticide resistance markers in Anopheles funestus from the Democratic Republic of the Congo using a targeted amplicon sequencing panel Scientific Reports |
| title | Detection of insecticide resistance markers in Anopheles funestus from the Democratic Republic of the Congo using a targeted amplicon sequencing panel |
| title_full | Detection of insecticide resistance markers in Anopheles funestus from the Democratic Republic of the Congo using a targeted amplicon sequencing panel |
| title_fullStr | Detection of insecticide resistance markers in Anopheles funestus from the Democratic Republic of the Congo using a targeted amplicon sequencing panel |
| title_full_unstemmed | Detection of insecticide resistance markers in Anopheles funestus from the Democratic Republic of the Congo using a targeted amplicon sequencing panel |
| title_short | Detection of insecticide resistance markers in Anopheles funestus from the Democratic Republic of the Congo using a targeted amplicon sequencing panel |
| title_sort | detection of insecticide resistance markers in anopheles funestus from the democratic republic of the congo using a targeted amplicon sequencing panel |
| url | https://doi.org/10.1038/s41598-023-44457-0 |
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