Seaweed Extract-Stimulated Priming in <i>Arabidopsis thaliana</i> and <i>Solanum lycopersicum</i>
Plant priming is an induced physiological state where plants are protected from biotic and abiotic stresses. Whether seaweed extracts promote priming is largely unknown as is the mechanism by which priming may occur. In this study, we examined the effect of a seaweed extract (SWE) on two distinct st...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/11/2476 |
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| author | Md Tohidul Islam Tony Arioli David M. Cahill |
| author_facet | Md Tohidul Islam Tony Arioli David M. Cahill |
| author_sort | Md Tohidul Islam |
| collection | DOAJ |
| description | Plant priming is an induced physiological state where plants are protected from biotic and abiotic stresses. Whether seaweed extracts promote priming is largely unknown as is the mechanism by which priming may occur. In this study, we examined the effect of a seaweed extract (SWE) on two distinct stages of plant priming (priming phase and post-challenge primed state) by characterising (i) plant gene expression responses using qRT-PCR and (ii) signal transduction responses by evaluating reactive oxygen species (ROS) production. The SWE is made from the brown algae <i>Ascophyllum nodosum</i> and <i>Durvillaea potatorum.</i> The priming phase was examined using both <i>Arabidopsis thaliana</i> and <i>Solanum lycopersicum</i>. At this stage, the SWE up-regulated key priming-related genes, such as those related to systemic acquired resistance (SAR) and activated the production of ROS. These responses were found to be temporal (lasting 3 days). The post-challenge primed state was examined using <i>A. thaliana</i> challenged with a root pathogen. Similarly, defence response-related genes, such as <i>PR1</i> and <i>NPR1</i>, were up-regulated and ROS production was activated (lasting 5 days). This study found that SWE induces plant priming-like responses by (i) up-regulating genes associated with plant defence responses and (ii) increasing production of ROS associated with signalling responses. |
| first_indexed | 2024-03-10T05:07:37Z |
| format | Article |
| id | doaj.art-1a85f91a8e694352bf17fdddca9f1173 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T05:07:37Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-1a85f91a8e694352bf17fdddca9f11732023-11-23T01:06:23ZengMDPI AGPlants2223-77472021-11-011011247610.3390/plants10112476Seaweed Extract-Stimulated Priming in <i>Arabidopsis thaliana</i> and <i>Solanum lycopersicum</i>Md Tohidul Islam0Tony Arioli1David M. Cahill2School of Life and Environmental Sciences, Deakin University Geelong Waurn Ponds Campus, Waurn Ponds, VIC 3216, AustraliaSchool of Life and Environmental Sciences, Deakin University Geelong Waurn Ponds Campus, Waurn Ponds, VIC 3216, AustraliaSchool of Life and Environmental Sciences, Deakin University Geelong Waurn Ponds Campus, Waurn Ponds, VIC 3216, AustraliaPlant priming is an induced physiological state where plants are protected from biotic and abiotic stresses. Whether seaweed extracts promote priming is largely unknown as is the mechanism by which priming may occur. In this study, we examined the effect of a seaweed extract (SWE) on two distinct stages of plant priming (priming phase and post-challenge primed state) by characterising (i) plant gene expression responses using qRT-PCR and (ii) signal transduction responses by evaluating reactive oxygen species (ROS) production. The SWE is made from the brown algae <i>Ascophyllum nodosum</i> and <i>Durvillaea potatorum.</i> The priming phase was examined using both <i>Arabidopsis thaliana</i> and <i>Solanum lycopersicum</i>. At this stage, the SWE up-regulated key priming-related genes, such as those related to systemic acquired resistance (SAR) and activated the production of ROS. These responses were found to be temporal (lasting 3 days). The post-challenge primed state was examined using <i>A. thaliana</i> challenged with a root pathogen. Similarly, defence response-related genes, such as <i>PR1</i> and <i>NPR1</i>, were up-regulated and ROS production was activated (lasting 5 days). This study found that SWE induces plant priming-like responses by (i) up-regulating genes associated with plant defence responses and (ii) increasing production of ROS associated with signalling responses.https://www.mdpi.com/2223-7747/10/11/2476seaweed<i>Ascophyllum nodosum</i><i>Durvillaea potatorum</i><i>Phytophthora cinnamomi</i><i>Arabidopsis thaliana</i>priming |
| spellingShingle | Md Tohidul Islam Tony Arioli David M. Cahill Seaweed Extract-Stimulated Priming in <i>Arabidopsis thaliana</i> and <i>Solanum lycopersicum</i> Plants seaweed <i>Ascophyllum nodosum</i> <i>Durvillaea potatorum</i> <i>Phytophthora cinnamomi</i> <i>Arabidopsis thaliana</i> priming |
| title | Seaweed Extract-Stimulated Priming in <i>Arabidopsis thaliana</i> and <i>Solanum lycopersicum</i> |
| title_full | Seaweed Extract-Stimulated Priming in <i>Arabidopsis thaliana</i> and <i>Solanum lycopersicum</i> |
| title_fullStr | Seaweed Extract-Stimulated Priming in <i>Arabidopsis thaliana</i> and <i>Solanum lycopersicum</i> |
| title_full_unstemmed | Seaweed Extract-Stimulated Priming in <i>Arabidopsis thaliana</i> and <i>Solanum lycopersicum</i> |
| title_short | Seaweed Extract-Stimulated Priming in <i>Arabidopsis thaliana</i> and <i>Solanum lycopersicum</i> |
| title_sort | seaweed extract stimulated priming in i arabidopsis thaliana i and i solanum lycopersicum i |
| topic | seaweed <i>Ascophyllum nodosum</i> <i>Durvillaea potatorum</i> <i>Phytophthora cinnamomi</i> <i>Arabidopsis thaliana</i> priming |
| url | https://www.mdpi.com/2223-7747/10/11/2476 |
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