High Electrochemiluminescence from Ru(bpy)32+ Embedded Metal–Organic Frameworks to Visualize Single Molecule Movement at the Cellular Membrane
Abstract Direct imaging of single‐molecule and its movement is of fundamental importance in biology, but challenging. Herein, aided by the nanoconfinement effect and resultant high reaction activity within metal–organic frameworks (MOFs), the designed Ru(bpy)32+ embedded MOF complex (RuMOFs) exhibit...
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| Format: | Article |
| Language: | English |
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Wiley
2022-12-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202204715 |
| _version_ | 1811291063068917760 |
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| author | Binxiao Li Xuedong Huang Yanwei Lu Zihui Fan Bin Li Dechen Jiang Neso Sojic Baohong Liu |
| author_facet | Binxiao Li Xuedong Huang Yanwei Lu Zihui Fan Bin Li Dechen Jiang Neso Sojic Baohong Liu |
| author_sort | Binxiao Li |
| collection | DOAJ |
| description | Abstract Direct imaging of single‐molecule and its movement is of fundamental importance in biology, but challenging. Herein, aided by the nanoconfinement effect and resultant high reaction activity within metal–organic frameworks (MOFs), the designed Ru(bpy)32+ embedded MOF complex (RuMOFs) exhibits bright electrochemiluminescence (ECL) emission permitting high‐quality imaging of ECL events at single molecule level. By labeling individual proteins of living cells with single RuMOFs, the distribution of membrane tyrosine‐protein‐kinase‐like7 (PTK7) proteins at low‐expressing cells is imaged via ECL. More importantly, the efficient capture of ECL photons generated inside the MOFs results in a stable ECL emission up to 1 h, allowing the in operando visualization of protein movements at the cellular membrane. As compared with the fluorescence observation, near‐zero ECL background surrounding the target protein with the ECL emitter gives a better contrast for the dynamic imaging of discrete protein movement. This achievement of single molecule ECL dynamic imaging using RuMOFs will provide a more effective nanoemitter to observe the distribution and motion of individual proteins at living cells. |
| first_indexed | 2024-04-13T04:22:50Z |
| format | Article |
| id | doaj.art-1a885322e07544e5b638f836f456e002 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-13T04:22:50Z |
| publishDate | 2022-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-1a885322e07544e5b638f836f456e0022022-12-22T03:02:39ZengWileyAdvanced Science2198-38442022-12-01935n/an/a10.1002/advs.202204715High Electrochemiluminescence from Ru(bpy)32+ Embedded Metal–Organic Frameworks to Visualize Single Molecule Movement at the Cellular MembraneBinxiao Li0Xuedong Huang1Yanwei Lu2Zihui Fan3Bin Li4Dechen Jiang5Neso Sojic6Baohong Liu7Department of Chemistry Shanghai Stomatological Hospital State Key Laboratory of Molecular Engineering of Polymers Fudan University Shanghai 200433 ChinaDepartment of Chemistry Shanghai Stomatological Hospital State Key Laboratory of Molecular Engineering of Polymers Fudan University Shanghai 200433 ChinaDepartment of Chemistry Shanghai Stomatological Hospital State Key Laboratory of Molecular Engineering of Polymers Fudan University Shanghai 200433 ChinaDepartment of Chemistry Shanghai Stomatological Hospital State Key Laboratory of Molecular Engineering of Polymers Fudan University Shanghai 200433 ChinaDepartment of Chemistry Shanghai Stomatological Hospital State Key Laboratory of Molecular Engineering of Polymers Fudan University Shanghai 200433 ChinaState Key Laboratory of Analytical Chemistry for Life and School of Chemistry and Chemical Engineering Nanjing University Nanjing Jiangsu 210093 ChinaBordeaux INP Institute of Molecular Science (ISM), and CNRS UMR 5255 University of Bordeaux Pessac 33607 FranceDepartment of Chemistry Shanghai Stomatological Hospital State Key Laboratory of Molecular Engineering of Polymers Fudan University Shanghai 200433 ChinaAbstract Direct imaging of single‐molecule and its movement is of fundamental importance in biology, but challenging. Herein, aided by the nanoconfinement effect and resultant high reaction activity within metal–organic frameworks (MOFs), the designed Ru(bpy)32+ embedded MOF complex (RuMOFs) exhibits bright electrochemiluminescence (ECL) emission permitting high‐quality imaging of ECL events at single molecule level. By labeling individual proteins of living cells with single RuMOFs, the distribution of membrane tyrosine‐protein‐kinase‐like7 (PTK7) proteins at low‐expressing cells is imaged via ECL. More importantly, the efficient capture of ECL photons generated inside the MOFs results in a stable ECL emission up to 1 h, allowing the in operando visualization of protein movements at the cellular membrane. As compared with the fluorescence observation, near‐zero ECL background surrounding the target protein with the ECL emitter gives a better contrast for the dynamic imaging of discrete protein movement. This achievement of single molecule ECL dynamic imaging using RuMOFs will provide a more effective nanoemitter to observe the distribution and motion of individual proteins at living cells.https://doi.org/10.1002/advs.202204715dynamic trackingmetal–organic frameworksnanoconfined‐enhanced emissionnanoemittersingle‐molecule ECL |
| spellingShingle | Binxiao Li Xuedong Huang Yanwei Lu Zihui Fan Bin Li Dechen Jiang Neso Sojic Baohong Liu High Electrochemiluminescence from Ru(bpy)32+ Embedded Metal–Organic Frameworks to Visualize Single Molecule Movement at the Cellular Membrane Advanced Science dynamic tracking metal–organic frameworks nanoconfined‐enhanced emission nanoemitter single‐molecule ECL |
| title | High Electrochemiluminescence from Ru(bpy)32+ Embedded Metal–Organic Frameworks to Visualize Single Molecule Movement at the Cellular Membrane |
| title_full | High Electrochemiluminescence from Ru(bpy)32+ Embedded Metal–Organic Frameworks to Visualize Single Molecule Movement at the Cellular Membrane |
| title_fullStr | High Electrochemiluminescence from Ru(bpy)32+ Embedded Metal–Organic Frameworks to Visualize Single Molecule Movement at the Cellular Membrane |
| title_full_unstemmed | High Electrochemiluminescence from Ru(bpy)32+ Embedded Metal–Organic Frameworks to Visualize Single Molecule Movement at the Cellular Membrane |
| title_short | High Electrochemiluminescence from Ru(bpy)32+ Embedded Metal–Organic Frameworks to Visualize Single Molecule Movement at the Cellular Membrane |
| title_sort | high electrochemiluminescence from ru bpy 32 embedded metal organic frameworks to visualize single molecule movement at the cellular membrane |
| topic | dynamic tracking metal–organic frameworks nanoconfined‐enhanced emission nanoemitter single‐molecule ECL |
| url | https://doi.org/10.1002/advs.202204715 |
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