MiR-638 suppresses the progression of oral squamous cell carcinoma through wnt/β-catenin pathway by targeting phospholipase D1
Objective The current study aimed to explore the function of miR-638 on the progression of oral squamous cell carcinoma (OSCC) and relevant molecular mechanisms.Methods Expression profile of miR-638 in OSCC tissues and cells was detected using quantitative real-time polymerase chain reaction (qRT-PC...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-12-01
|
| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1647222 |
| _version_ | 1817981438599888896 |
|---|---|
| author | Kai-Liang Tang Han-Ying Tang Yi- Du Tian Tian Shi-Jiang Xiong |
| author_facet | Kai-Liang Tang Han-Ying Tang Yi- Du Tian Tian Shi-Jiang Xiong |
| author_sort | Kai-Liang Tang |
| collection | DOAJ |
| description | Objective The current study aimed to explore the function of miR-638 on the progression of oral squamous cell carcinoma (OSCC) and relevant molecular mechanisms.Methods Expression profile of miR-638 in OSCC tissues and cells was detected using quantitative real-time polymerase chain reaction (qRT-PCR) method. Chi-square test was performed to estimate the relationship between miR-638 and clinical parameters of OSCC cases. Cell viability and motility abilities were estimated using MTT and transwell assays, respectively. Potential targets of miR-638 in OSCC were identified through bioinformatics analysis and luciferase reporter assay.Results MiR-638 exhibited decreased expression in OSCC tissues and cells, compared to non-cancerous controls (P < .05 for both). Moreover, its down-regulation was closely correlated with lymph node metastasis (P = .044) and TNM stages (P = .001). Enforced miR-638 expression reduced cell proliferation, migration and invasion, while its knockdown exhibited opposite effects. Phospholipase D1 (PLD1) was confirmed as a target of miR-638 in OSCC. MiR-638 could inhibit wnt/β-catenin pathway through targeting PLD1, thus realizing its anti-tumour action in OSCC.Conclusion MiR-638 may be a tumour suppressor in OSCC by targeting PLD1/Wnt/β-catenin pathway. |
| first_indexed | 2024-04-13T23:07:00Z |
| format | Article |
| id | doaj.art-1a88945de12941128572a1ecdf2340b0 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-13T23:07:00Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-1a88945de12941128572a1ecdf2340b02022-12-22T02:25:40ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014713278328510.1080/21691401.2019.1647222MiR-638 suppresses the progression of oral squamous cell carcinoma through wnt/β-catenin pathway by targeting phospholipase D1Kai-Liang Tang0Han-Ying Tang1Yi- Du2Tian Tian3Shi-Jiang Xiong4Department of VIP Center and Shandong Provincial Key Laboratory of Oral Biomedicine, School and Hospital of Stomatology, Shandong University, Jinan, Shandong, ChinaDepartment of Oral prosthology, Jinan Stomatological Hospital, Jinan, Shandong, ChinaDepartment of Endodontics, Jinan Stomatological Hospital, Jinan, Shandong, ChinaDepartment of Stomatology, Affiliated Hospital of Binzhou Medical College, Binzhou, Shandong, ChinaDepartment of VIP Center and Shandong Provincial Key Laboratory of Oral Biomedicine, School and Hospital of Stomatology, Shandong University, Jinan, Shandong, ChinaObjective The current study aimed to explore the function of miR-638 on the progression of oral squamous cell carcinoma (OSCC) and relevant molecular mechanisms.Methods Expression profile of miR-638 in OSCC tissues and cells was detected using quantitative real-time polymerase chain reaction (qRT-PCR) method. Chi-square test was performed to estimate the relationship between miR-638 and clinical parameters of OSCC cases. Cell viability and motility abilities were estimated using MTT and transwell assays, respectively. Potential targets of miR-638 in OSCC were identified through bioinformatics analysis and luciferase reporter assay.Results MiR-638 exhibited decreased expression in OSCC tissues and cells, compared to non-cancerous controls (P < .05 for both). Moreover, its down-regulation was closely correlated with lymph node metastasis (P = .044) and TNM stages (P = .001). Enforced miR-638 expression reduced cell proliferation, migration and invasion, while its knockdown exhibited opposite effects. Phospholipase D1 (PLD1) was confirmed as a target of miR-638 in OSCC. MiR-638 could inhibit wnt/β-catenin pathway through targeting PLD1, thus realizing its anti-tumour action in OSCC.Conclusion MiR-638 may be a tumour suppressor in OSCC by targeting PLD1/Wnt/β-catenin pathway.https://www.tandfonline.com/doi/10.1080/21691401.2019.1647222Oral squamous cell carcinomamicroRNAsmiR-638wnt/β-catenin pathwayPLD1 |
| spellingShingle | Kai-Liang Tang Han-Ying Tang Yi- Du Tian Tian Shi-Jiang Xiong MiR-638 suppresses the progression of oral squamous cell carcinoma through wnt/β-catenin pathway by targeting phospholipase D1 Artificial Cells, Nanomedicine, and Biotechnology Oral squamous cell carcinoma microRNAs miR-638 wnt/β-catenin pathway PLD1 |
| title | MiR-638 suppresses the progression of oral squamous cell carcinoma through wnt/β-catenin pathway by targeting phospholipase D1 |
| title_full | MiR-638 suppresses the progression of oral squamous cell carcinoma through wnt/β-catenin pathway by targeting phospholipase D1 |
| title_fullStr | MiR-638 suppresses the progression of oral squamous cell carcinoma through wnt/β-catenin pathway by targeting phospholipase D1 |
| title_full_unstemmed | MiR-638 suppresses the progression of oral squamous cell carcinoma through wnt/β-catenin pathway by targeting phospholipase D1 |
| title_short | MiR-638 suppresses the progression of oral squamous cell carcinoma through wnt/β-catenin pathway by targeting phospholipase D1 |
| title_sort | mir 638 suppresses the progression of oral squamous cell carcinoma through wnt β catenin pathway by targeting phospholipase d1 |
| topic | Oral squamous cell carcinoma microRNAs miR-638 wnt/β-catenin pathway PLD1 |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1647222 |
| work_keys_str_mv | AT kailiangtang mir638suppressestheprogressionoforalsquamouscellcarcinomathroughwntbcateninpathwaybytargetingphospholipased1 AT hanyingtang mir638suppressestheprogressionoforalsquamouscellcarcinomathroughwntbcateninpathwaybytargetingphospholipased1 AT yidu mir638suppressestheprogressionoforalsquamouscellcarcinomathroughwntbcateninpathwaybytargetingphospholipased1 AT tiantian mir638suppressestheprogressionoforalsquamouscellcarcinomathroughwntbcateninpathwaybytargetingphospholipased1 AT shijiangxiong mir638suppressestheprogressionoforalsquamouscellcarcinomathroughwntbcateninpathwaybytargetingphospholipased1 |