Suitability of Ex Vivo-Expanded Microtic Perichondrocytes for Auricular Reconstruction
Tissue engineering (TE) techniques offer solutions for tissue regeneration but require large quantities of cells. For microtia patients, TE methods represent a unique opportunity for therapies with low donor-site morbidity and reliance on the surgeon’s individual expertise. Microtia-derived chondroc...
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MDPI AG
2024-01-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/13/2/141 |
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| author | Yvonne Jakob Johann Kern David Gvaramia Philipp Fisch Ralph Magritz Sven Reutter Nicole Rotter |
| author_facet | Yvonne Jakob Johann Kern David Gvaramia Philipp Fisch Ralph Magritz Sven Reutter Nicole Rotter |
| author_sort | Yvonne Jakob |
| collection | DOAJ |
| description | Tissue engineering (TE) techniques offer solutions for tissue regeneration but require large quantities of cells. For microtia patients, TE methods represent a unique opportunity for therapies with low donor-site morbidity and reliance on the surgeon’s individual expertise. Microtia-derived chondrocytes and perichondrocytes are considered a valuable cell source for autologous reconstruction of the pinna. The aim of this study was to investigate the suitability of perichondrocytes from microtia patients for autologous reconstruction in comparison to healthy perichondrocytes and microtia chondrocytes. Perichondrocytes were isolated via two different methods: explant culture and enzymatic digestion. The isolated cells were analyzed in vitro for their chondrogenic cell properties. We examined migration activity, colony-forming ability, expression of mesenchymal stem cell markers, and gene expression profile. We found that microtic perichondrocytes exhibit similar chondrogenic properties compared to chondrocytes in vitro. We investigated the behavior in three-dimensional cell cultures (spheroids and scaffold-based 3D cell cultures) and assessed the expression of cartilage-specific proteins via immunohistochemistry, e.g., collagen II, which was detected in all samples. Our results show that perichondrocytes from microtia patients are comparable to healthy perichondrocytes and chondrocytes in terms of chondrogenic cell properties and could therefore be a promising cell source for auricular reconstruction. |
| first_indexed | 2024-03-08T09:55:33Z |
| format | Article |
| id | doaj.art-1a8904bfcd3f4d96a6508283ae1e853f |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-08T09:55:33Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-1a8904bfcd3f4d96a6508283ae1e853f2024-01-29T13:50:20ZengMDPI AGCells2073-44092024-01-0113214110.3390/cells13020141Suitability of Ex Vivo-Expanded Microtic Perichondrocytes for Auricular ReconstructionYvonne Jakob0Johann Kern1David Gvaramia2Philipp Fisch3Ralph Magritz4Sven Reutter5Nicole Rotter6Department of Otorhinolaryngology Head and Neck Surgery, Medical Faculty Mannheim, Heidelberg University, Theodor-Kutzer-Ufer 1-3, D-68167 Mannheim, GermanyDepartment of Otorhinolaryngology Head and Neck Surgery, Medical Faculty Mannheim, Heidelberg University, Theodor-Kutzer-Ufer 1-3, D-68167 Mannheim, GermanyDepartment of Otorhinolaryngology Head and Neck Surgery, Medical Faculty Mannheim, Heidelberg University, Theodor-Kutzer-Ufer 1-3, D-68167 Mannheim, GermanyTissue Engineering and Biofabrication Laboratory, Department of Health Sciences & Technology, ETH Zurich, Otto-Stern-Weg 7, CH-8093 Zurich, SwitzerlandClinic for Otorhinolaryngology, Oberhavel-Kliniken GmbH, Klinik Henningsdorf, Marwitzer Strasse 91, D-16761 Henningsdorf, GermanyDepartment of Otorhinolaryngology Head and Neck Surgery, Medical Faculty Mannheim, Heidelberg University, Theodor-Kutzer-Ufer 1-3, D-68167 Mannheim, GermanyDepartment of Otorhinolaryngology Head and Neck Surgery, Medical Faculty Mannheim, Heidelberg University, Theodor-Kutzer-Ufer 1-3, D-68167 Mannheim, GermanyTissue engineering (TE) techniques offer solutions for tissue regeneration but require large quantities of cells. For microtia patients, TE methods represent a unique opportunity for therapies with low donor-site morbidity and reliance on the surgeon’s individual expertise. Microtia-derived chondrocytes and perichondrocytes are considered a valuable cell source for autologous reconstruction of the pinna. The aim of this study was to investigate the suitability of perichondrocytes from microtia patients for autologous reconstruction in comparison to healthy perichondrocytes and microtia chondrocytes. Perichondrocytes were isolated via two different methods: explant culture and enzymatic digestion. The isolated cells were analyzed in vitro for their chondrogenic cell properties. We examined migration activity, colony-forming ability, expression of mesenchymal stem cell markers, and gene expression profile. We found that microtic perichondrocytes exhibit similar chondrogenic properties compared to chondrocytes in vitro. We investigated the behavior in three-dimensional cell cultures (spheroids and scaffold-based 3D cell cultures) and assessed the expression of cartilage-specific proteins via immunohistochemistry, e.g., collagen II, which was detected in all samples. Our results show that perichondrocytes from microtia patients are comparable to healthy perichondrocytes and chondrocytes in terms of chondrogenic cell properties and could therefore be a promising cell source for auricular reconstruction.https://www.mdpi.com/2073-4409/13/2/141perichondrocytesmicrotiatissue engineeringauricular reconstruction3D cell culturesspheroids |
| spellingShingle | Yvonne Jakob Johann Kern David Gvaramia Philipp Fisch Ralph Magritz Sven Reutter Nicole Rotter Suitability of Ex Vivo-Expanded Microtic Perichondrocytes for Auricular Reconstruction Cells perichondrocytes microtia tissue engineering auricular reconstruction 3D cell cultures spheroids |
| title | Suitability of Ex Vivo-Expanded Microtic Perichondrocytes for Auricular Reconstruction |
| title_full | Suitability of Ex Vivo-Expanded Microtic Perichondrocytes for Auricular Reconstruction |
| title_fullStr | Suitability of Ex Vivo-Expanded Microtic Perichondrocytes for Auricular Reconstruction |
| title_full_unstemmed | Suitability of Ex Vivo-Expanded Microtic Perichondrocytes for Auricular Reconstruction |
| title_short | Suitability of Ex Vivo-Expanded Microtic Perichondrocytes for Auricular Reconstruction |
| title_sort | suitability of ex vivo expanded microtic perichondrocytes for auricular reconstruction |
| topic | perichondrocytes microtia tissue engineering auricular reconstruction 3D cell cultures spheroids |
| url | https://www.mdpi.com/2073-4409/13/2/141 |
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