Characteristic and Functional Study of Intersex, a Gene Related to Female Fertility in Bemisia tabaci
The intersex (ix) gene acts in concert with doublesex (dsx) at the end of the sex determination hierarchy to control somatic sexual differentiation in Drosophila melanogaster. Here, we report the Drosophila ix homolog in Bemisia tabaci (Btix) with differential splicing events. Four isoforms were fou...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.00055/full |
| _version_ | 1818066411278303232 |
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| author | Yating Liu Jinjian Yang Zhijia Huo Shaoli Wang Qingjun Wu Xuguo Zhou Wen Xie Youjun Zhang |
| author_facet | Yating Liu Jinjian Yang Zhijia Huo Shaoli Wang Qingjun Wu Xuguo Zhou Wen Xie Youjun Zhang |
| author_sort | Yating Liu |
| collection | DOAJ |
| description | The intersex (ix) gene acts in concert with doublesex (dsx) at the end of the sex determination hierarchy to control somatic sexual differentiation in Drosophila melanogaster. Here, we report the Drosophila ix homolog in Bemisia tabaci (Btix) with differential splicing events. Four isoforms were found in B. tabaci adults, including two sex-specific transcripts (BtixF and BtixM). Knockdown of Btix had no measurable effects on female morphological phenotypes but reduced the expression of the vitellogenin gene and resulted in the production of significantly fewer eggs, a lower eclosion rate, and a shorter body size of female progeny in comparison with control females. These results increase our understanding of the genes underlying sex determination in B. tabaci and reveal a potential target for RNA interference-based pest management. |
| first_indexed | 2024-12-10T15:07:21Z |
| format | Article |
| id | doaj.art-1a89c4f233f14aa9bd84a06129783d2d |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-10T15:07:21Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-1a89c4f233f14aa9bd84a06129783d2d2022-12-22T01:44:01ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-02-011110.3389/fphys.2020.00055508463Characteristic and Functional Study of Intersex, a Gene Related to Female Fertility in Bemisia tabaciYating Liu0Jinjian Yang1Zhijia Huo2Shaoli Wang3Qingjun Wu4Xuguo Zhou5Wen Xie6Youjun Zhang7Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Entomology, University of Kentucky, Lexington, KY, United StatesDepartment of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaThe intersex (ix) gene acts in concert with doublesex (dsx) at the end of the sex determination hierarchy to control somatic sexual differentiation in Drosophila melanogaster. Here, we report the Drosophila ix homolog in Bemisia tabaci (Btix) with differential splicing events. Four isoforms were found in B. tabaci adults, including two sex-specific transcripts (BtixF and BtixM). Knockdown of Btix had no measurable effects on female morphological phenotypes but reduced the expression of the vitellogenin gene and resulted in the production of significantly fewer eggs, a lower eclosion rate, and a shorter body size of female progeny in comparison with control females. These results increase our understanding of the genes underlying sex determination in B. tabaci and reveal a potential target for RNA interference-based pest management.https://www.frontiersin.org/article/10.3389/fphys.2020.00055/fullBemisia tabaciintersexRNA interferencesex determinationpest control |
| spellingShingle | Yating Liu Jinjian Yang Zhijia Huo Shaoli Wang Qingjun Wu Xuguo Zhou Wen Xie Youjun Zhang Characteristic and Functional Study of Intersex, a Gene Related to Female Fertility in Bemisia tabaci Frontiers in Physiology Bemisia tabaci intersex RNA interference sex determination pest control |
| title | Characteristic and Functional Study of Intersex, a Gene Related to Female Fertility in Bemisia tabaci |
| title_full | Characteristic and Functional Study of Intersex, a Gene Related to Female Fertility in Bemisia tabaci |
| title_fullStr | Characteristic and Functional Study of Intersex, a Gene Related to Female Fertility in Bemisia tabaci |
| title_full_unstemmed | Characteristic and Functional Study of Intersex, a Gene Related to Female Fertility in Bemisia tabaci |
| title_short | Characteristic and Functional Study of Intersex, a Gene Related to Female Fertility in Bemisia tabaci |
| title_sort | characteristic and functional study of intersex a gene related to female fertility in bemisia tabaci |
| topic | Bemisia tabaci intersex RNA interference sex determination pest control |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.00055/full |
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