Triglyceride profiling in adipose tissues from obese insulin sensitive, insulin resistant and type 2 diabetes mellitus individuals
Abstract Background Lipid intermediates produced during triacylglycerols (TAGs) synthesis and lipolysis in adipocytes interfere with the intracellular insulin signaling pathway and development of insulin resistance. This study aims to compare TAG species and their fatty acid composition in adipose t...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12967-018-1548-x |
| _version_ | 1818522067376537600 |
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| author | Haya Al-Sulaiti Ilhame Diboun Sameem Banu Mohamed Al-Emadi Parvaneh Amani Thomas M. Harvey Alex S. Dömling Aishah Latiff Mohamed A. Elrayess |
| author_facet | Haya Al-Sulaiti Ilhame Diboun Sameem Banu Mohamed Al-Emadi Parvaneh Amani Thomas M. Harvey Alex S. Dömling Aishah Latiff Mohamed A. Elrayess |
| author_sort | Haya Al-Sulaiti |
| collection | DOAJ |
| description | Abstract Background Lipid intermediates produced during triacylglycerols (TAGs) synthesis and lipolysis in adipocytes interfere with the intracellular insulin signaling pathway and development of insulin resistance. This study aims to compare TAG species and their fatty acid composition in adipose tissues from insulin sensitive (IS), insulin resistant (IR) and type 2 diabetes mellitus (T2DM) obese individuals. Methods Human subcutaneous and omental adipose tissue biopsies were obtained from 64 clinically characterized obese individuals during weight reduction surgery. TAGs were extracted from the adipose tissues using the Bligh and Dyer method, then were subjected to non-aqueous reverse phase ultra-high performance liquid chromatography and full scan mass spectrometry acquisition and data dependent MS/MS on LTQ dual cell linear ion trap. TAGs and their fatty acid contents were identified and compared between IS, IR and T2DM individuals and their levels were correlated with metabolic traits of participants and the adipogenic potential of preadipocyte cultures established from their adipose tissues. Results Data revealed 76 unique TAG species in adipose tissues identified based on their exact mass. Analysis of TAG levels revealed a number of TAGs that were significantly altered with disease progression including C46:4, C48:5, C48:4, C38:1, C50:3, C40:2, C56:3, C56:4, C56:7 and C58:7. Enrichment analysis revealed C12:0 fatty acid to be associated with TAGs least abundant in T2DM whereas C18:3 was found in both depleted and enriched TAGs in T2DM. Significant correlations of various adipose tissue-derived TAG species and metabolic traits were observed, including age and body mass index, systemic total cholesterol, TAGs, and interleukin-6 in addition to adipogenic potential of preadipocytes derived from the same adipose tissues. Conclusion Pilot data suggest that adipose tissues from obese IR and T2DM individuals exhibit TAG-specific signatures that may contribute to their increased risk compared to their IS counterparts. Future experiments are warranted to investigate the functional relevance of these specific lipidomic profiles. |
| first_indexed | 2024-12-11T05:28:26Z |
| format | Article |
| id | doaj.art-1a8c79f181534d349a72bde390f4b8d5 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-11T05:28:26Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-1a8c79f181534d349a72bde390f4b8d52022-12-22T01:19:31ZengBMCJournal of Translational Medicine1479-58762018-06-0116111310.1186/s12967-018-1548-xTriglyceride profiling in adipose tissues from obese insulin sensitive, insulin resistant and type 2 diabetes mellitus individualsHaya Al-Sulaiti0Ilhame Diboun1Sameem Banu2Mohamed Al-Emadi3Parvaneh Amani4Thomas M. Harvey5Alex S. Dömling6Aishah Latiff7Mohamed A. Elrayess8Toxicology and Multipurpose Lab, Anti Doping Laboratory QatarDepartment of Economics, Mathematics and Statistics, Birkbeck, University of LondonToxicology and Multipurpose Lab, Anti Doping Laboratory QatarGeneral Surgery Department, Al-Emdi HospitalGeneral Surgery Department, Al-Emdi HospitalToxicology and Multipurpose Lab, Anti Doping Laboratory QatarDepartment of Drug Design, University of GroningenToxicology and Multipurpose Lab, Anti Doping Laboratory QatarToxicology and Multipurpose Lab, Anti Doping Laboratory QatarAbstract Background Lipid intermediates produced during triacylglycerols (TAGs) synthesis and lipolysis in adipocytes interfere with the intracellular insulin signaling pathway and development of insulin resistance. This study aims to compare TAG species and their fatty acid composition in adipose tissues from insulin sensitive (IS), insulin resistant (IR) and type 2 diabetes mellitus (T2DM) obese individuals. Methods Human subcutaneous and omental adipose tissue biopsies were obtained from 64 clinically characterized obese individuals during weight reduction surgery. TAGs were extracted from the adipose tissues using the Bligh and Dyer method, then were subjected to non-aqueous reverse phase ultra-high performance liquid chromatography and full scan mass spectrometry acquisition and data dependent MS/MS on LTQ dual cell linear ion trap. TAGs and their fatty acid contents were identified and compared between IS, IR and T2DM individuals and their levels were correlated with metabolic traits of participants and the adipogenic potential of preadipocyte cultures established from their adipose tissues. Results Data revealed 76 unique TAG species in adipose tissues identified based on their exact mass. Analysis of TAG levels revealed a number of TAGs that were significantly altered with disease progression including C46:4, C48:5, C48:4, C38:1, C50:3, C40:2, C56:3, C56:4, C56:7 and C58:7. Enrichment analysis revealed C12:0 fatty acid to be associated with TAGs least abundant in T2DM whereas C18:3 was found in both depleted and enriched TAGs in T2DM. Significant correlations of various adipose tissue-derived TAG species and metabolic traits were observed, including age and body mass index, systemic total cholesterol, TAGs, and interleukin-6 in addition to adipogenic potential of preadipocytes derived from the same adipose tissues. Conclusion Pilot data suggest that adipose tissues from obese IR and T2DM individuals exhibit TAG-specific signatures that may contribute to their increased risk compared to their IS counterparts. Future experiments are warranted to investigate the functional relevance of these specific lipidomic profiles.http://link.springer.com/article/10.1186/s12967-018-1548-xLipidomicsAdipose tissueTriaclyglycerolsInsulin sensitivityInsulin resistanceType 2 diabetes mellitus |
| spellingShingle | Haya Al-Sulaiti Ilhame Diboun Sameem Banu Mohamed Al-Emadi Parvaneh Amani Thomas M. Harvey Alex S. Dömling Aishah Latiff Mohamed A. Elrayess Triglyceride profiling in adipose tissues from obese insulin sensitive, insulin resistant and type 2 diabetes mellitus individuals Journal of Translational Medicine Lipidomics Adipose tissue Triaclyglycerols Insulin sensitivity Insulin resistance Type 2 diabetes mellitus |
| title | Triglyceride profiling in adipose tissues from obese insulin sensitive, insulin resistant and type 2 diabetes mellitus individuals |
| title_full | Triglyceride profiling in adipose tissues from obese insulin sensitive, insulin resistant and type 2 diabetes mellitus individuals |
| title_fullStr | Triglyceride profiling in adipose tissues from obese insulin sensitive, insulin resistant and type 2 diabetes mellitus individuals |
| title_full_unstemmed | Triglyceride profiling in adipose tissues from obese insulin sensitive, insulin resistant and type 2 diabetes mellitus individuals |
| title_short | Triglyceride profiling in adipose tissues from obese insulin sensitive, insulin resistant and type 2 diabetes mellitus individuals |
| title_sort | triglyceride profiling in adipose tissues from obese insulin sensitive insulin resistant and type 2 diabetes mellitus individuals |
| topic | Lipidomics Adipose tissue Triaclyglycerols Insulin sensitivity Insulin resistance Type 2 diabetes mellitus |
| url | http://link.springer.com/article/10.1186/s12967-018-1548-x |
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