Candidatus Desulforudis audaxviator dominates a 975 m deep groundwater community in central Sweden
Abstract The continental bedrock contains groundwater-bearing fractures that are home to microbial populations that are vital in mediating the Earth’s biogeochemical cycles. However, their diversity is poorly understood due to the difficulty of obtaining samples from this environment. Here, a ground...
| Päätekijät: | , , , , , , |
|---|---|
| Aineistotyyppi: | Artikkeli |
| Kieli: | English |
| Julkaistu: |
Nature Portfolio
2024-10-01
|
| Sarja: | Communications Biology |
| Linkit: | https://doi.org/10.1038/s42003-024-07027-2 |
| _version_ | 1827059683551084544 |
|---|---|
| author | George Westmeijer Femke van Dam Riikka Kietäväinen Carolina González-Rosales Stefan Bertilsson Henrik Drake Mark Dopson |
| author_facet | George Westmeijer Femke van Dam Riikka Kietäväinen Carolina González-Rosales Stefan Bertilsson Henrik Drake Mark Dopson |
| author_sort | George Westmeijer |
| collection | DOAJ |
| description | Abstract The continental bedrock contains groundwater-bearing fractures that are home to microbial populations that are vital in mediating the Earth’s biogeochemical cycles. However, their diversity is poorly understood due to the difficulty of obtaining samples from this environment. Here, a groundwater-bearing fracture at 975 m depth was isolated by employing packers in order to characterize the microbial community via metagenomes combined with prokaryotic and eukaryotic marker genes (16S and 18S ribosomal RNA gene). Genome-resolved analyses revealed a community dominated by sulfate-reducing Bacillota, predominantly represented by Candidatus Desulforudis audaxviator and with Wood-Ljungdahl as the most prevalent pathway for inorganic carbon fixation. Moreover, the eukaryotic community had a considerable diversity and was comprised of mainly flatworms, chlorophytes, crustaceans, ochrophytes, and fungi. These findings support the important role of the Bacillota, with the sulfate reducer Candidatus Desulforudis audaxviator as its main representative, as primary producers in the often energy-limited groundwaters of the continental subsurface. |
| first_indexed | 2025-02-18T18:46:18Z |
| format | Article |
| id | doaj.art-1a8f29bcce1e4a0f9eb64cf38d9025d0 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2025-02-18T18:46:18Z |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-1a8f29bcce1e4a0f9eb64cf38d9025d02024-10-20T11:41:37ZengNature PortfolioCommunications Biology2399-36422024-10-017111010.1038/s42003-024-07027-2Candidatus Desulforudis audaxviator dominates a 975 m deep groundwater community in central SwedenGeorge Westmeijer0Femke van Dam1Riikka Kietäväinen2Carolina González-Rosales3Stefan Bertilsson4Henrik Drake5Mark Dopson6Centre for Ecology and Evolution in Microbial model Systems (EEMiS), Linnaeus University, Stuvaregatan 4Department of Biology and Environmental Sciences, Linnaeus University, Stuvaregatan 4Geological Survey of FinlandCentre for Ecology and Evolution in Microbial model Systems (EEMiS), Linnaeus University, Stuvaregatan 4Department of Aquatic Sciences and Assessment, Swedish University of Agricultural SciencesDepartment of Biology and Environmental Sciences, Linnaeus University, Stuvaregatan 4Centre for Ecology and Evolution in Microbial model Systems (EEMiS), Linnaeus University, Stuvaregatan 4Abstract The continental bedrock contains groundwater-bearing fractures that are home to microbial populations that are vital in mediating the Earth’s biogeochemical cycles. However, their diversity is poorly understood due to the difficulty of obtaining samples from this environment. Here, a groundwater-bearing fracture at 975 m depth was isolated by employing packers in order to characterize the microbial community via metagenomes combined with prokaryotic and eukaryotic marker genes (16S and 18S ribosomal RNA gene). Genome-resolved analyses revealed a community dominated by sulfate-reducing Bacillota, predominantly represented by Candidatus Desulforudis audaxviator and with Wood-Ljungdahl as the most prevalent pathway for inorganic carbon fixation. Moreover, the eukaryotic community had a considerable diversity and was comprised of mainly flatworms, chlorophytes, crustaceans, ochrophytes, and fungi. These findings support the important role of the Bacillota, with the sulfate reducer Candidatus Desulforudis audaxviator as its main representative, as primary producers in the often energy-limited groundwaters of the continental subsurface.https://doi.org/10.1038/s42003-024-07027-2 |
| spellingShingle | George Westmeijer Femke van Dam Riikka Kietäväinen Carolina González-Rosales Stefan Bertilsson Henrik Drake Mark Dopson Candidatus Desulforudis audaxviator dominates a 975 m deep groundwater community in central Sweden Communications Biology |
| title | Candidatus Desulforudis audaxviator dominates a 975 m deep groundwater community in central Sweden |
| title_full | Candidatus Desulforudis audaxviator dominates a 975 m deep groundwater community in central Sweden |
| title_fullStr | Candidatus Desulforudis audaxviator dominates a 975 m deep groundwater community in central Sweden |
| title_full_unstemmed | Candidatus Desulforudis audaxviator dominates a 975 m deep groundwater community in central Sweden |
| title_short | Candidatus Desulforudis audaxviator dominates a 975 m deep groundwater community in central Sweden |
| title_sort | candidatus desulforudis audaxviator dominates a 975 m deep groundwater community in central sweden |
| url | https://doi.org/10.1038/s42003-024-07027-2 |
| work_keys_str_mv | AT georgewestmeijer candidatusdesulforudisaudaxviatordominatesa975mdeepgroundwatercommunityincentralsweden AT femkevandam candidatusdesulforudisaudaxviatordominatesa975mdeepgroundwatercommunityincentralsweden AT riikkakietavainen candidatusdesulforudisaudaxviatordominatesa975mdeepgroundwatercommunityincentralsweden AT carolinagonzalezrosales candidatusdesulforudisaudaxviatordominatesa975mdeepgroundwatercommunityincentralsweden AT stefanbertilsson candidatusdesulforudisaudaxviatordominatesa975mdeepgroundwatercommunityincentralsweden AT henrikdrake candidatusdesulforudisaudaxviatordominatesa975mdeepgroundwatercommunityincentralsweden AT markdopson candidatusdesulforudisaudaxviatordominatesa975mdeepgroundwatercommunityincentralsweden |