OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS
<p class="MsoNormal" style="margin: 0cm 0cm 0pt; line-height: normal; text-align: justify; mso-layout-grid-align: none;"> Most well-known enzymatic methods have been employed in the bioanalysis of food, required by law in a number of countries. Food is a complex sam...
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Format: | Article |
Language: | English |
Published: |
Universidade Estadual Paulista
2008-11-01
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Series: | Alimentos e Nutrição |
Subjects: | |
Online Access: | http://serv-bib.fcfar.unesp.br/seer/index.php/alimentos/article/view/141 |
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author | L. A. CAMARGO M. F. S. PERES E. A. L. GATTÁS |
author_facet | L. A. CAMARGO M. F. S. PERES E. A. L. GATTÁS |
author_sort | L. A. CAMARGO |
collection | DOAJ |
description | <p class="MsoNormal" style="margin: 0cm 0cm 0pt; line-height: normal; text-align: justify; mso-layout-grid-align: none;"> Most well-known enzymatic methods have been employed in the bioanalysis of food, required by law in a number of countries. Food is a complex sample and hence difficult to analyze. In food analysis, there are many different types of sample, such as: clear or turbid, colorless or colored, neutral or acid, liquids, pastes and solids, that can be analyzed by enzymatic methods. The oxidase - peroxidase system may be used for specific analyses. In the reaction catalyzed by the oxidase, hydrogen is transferred from the substrate to oxygen and hydrogen peroxide is formed as an intermediate. Hydrogen peroxide then reacts with a leuco dye in the presence of the enzyme peroxidase and a color develops which is measured in the visible spectrum. This article is a review of two enzymes, peroxidase (POD) and glycerol-3-phosphate oxidase (GPO), pointing out their principal sources, characteristics and applications. |
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format | Article |
id | doaj.art-1a910bf0e1db4c95901f23bea98404b5 |
institution | Directory Open Access Journal |
issn | 0103-4235 2179-4448 |
language | English |
last_indexed | 2024-12-18T10:21:49Z |
publishDate | 2008-11-01 |
publisher | Universidade Estadual Paulista |
record_format | Article |
series | Alimentos e Nutrição |
spelling | doaj.art-1a910bf0e1db4c95901f23bea98404b52022-12-21T21:11:06ZengUniversidade Estadual PaulistaAlimentos e Nutrição0103-42352179-44482008-11-01181101109OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLSL. A. CAMARGOM. F. S. PERESE. A. L. GATTÁS<p class="MsoNormal" style="margin: 0cm 0cm 0pt; line-height: normal; text-align: justify; mso-layout-grid-align: none;"> Most well-known enzymatic methods have been employed in the bioanalysis of food, required by law in a number of countries. Food is a complex sample and hence difficult to analyze. In food analysis, there are many different types of sample, such as: clear or turbid, colorless or colored, neutral or acid, liquids, pastes and solids, that can be analyzed by enzymatic methods. The oxidase - peroxidase system may be used for specific analyses. In the reaction catalyzed by the oxidase, hydrogen is transferred from the substrate to oxygen and hydrogen peroxide is formed as an intermediate. Hydrogen peroxide then reacts with a leuco dye in the presence of the enzyme peroxidase and a color develops which is measured in the visible spectrum. This article is a review of two enzymes, peroxidase (POD) and glycerol-3-phosphate oxidase (GPO), pointing out their principal sources, characteristics and applications.http://serv-bib.fcfar.unesp.br/seer/index.php/alimentos/article/view/141Enzymesglycerol-3-phosphate oxidaseapplicationsperoxidaseapplications |
spellingShingle | L. A. CAMARGO M. F. S. PERES E. A. L. GATTÁS OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS Alimentos e Nutrição Enzymes glycerol-3-phosphate oxidase applications peroxidase applications |
title | OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS |
title_full | OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS |
title_fullStr | OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS |
title_full_unstemmed | OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS |
title_short | OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS |
title_sort | oxidoreductases for bioanalysis and food controls |
topic | Enzymes glycerol-3-phosphate oxidase applications peroxidase applications |
url | http://serv-bib.fcfar.unesp.br/seer/index.php/alimentos/article/view/141 |
work_keys_str_mv | AT lacamargo oxidoreductasesforbioanalysisandfoodcontrols AT mfsperes oxidoreductasesforbioanalysisandfoodcontrols AT ealgattas oxidoreductasesforbioanalysisandfoodcontrols |