OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS

<p class="MsoNormal" style="margin: 0cm 0cm 0pt; line-height: normal; text-align: justify; mso-layout-grid-align: none;"> Most well-known enzymatic methods have been employed in the bioanalysis of food, required by law in a number of countries. Food is a complex sam...

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Main Authors: L. A. CAMARGO, M. F. S. PERES, E. A. L. GATTÁS
Format: Article
Language:English
Published: Universidade Estadual Paulista 2008-11-01
Series:Alimentos e Nutrição
Subjects:
Online Access:http://serv-bib.fcfar.unesp.br/seer/index.php/alimentos/article/view/141
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author L. A. CAMARGO
M. F. S. PERES
E. A. L. GATTÁS
author_facet L. A. CAMARGO
M. F. S. PERES
E. A. L. GATTÁS
author_sort L. A. CAMARGO
collection DOAJ
description <p class="MsoNormal" style="margin: 0cm 0cm 0pt; line-height: normal; text-align: justify; mso-layout-grid-align: none;"> Most well-known enzymatic methods have been employed in the bioanalysis of food, required by law in a number of countries. Food is a complex sample and hence difficult to analyze. In food analysis, there are many different types of sample, such as: clear or turbid, colorless or colored, neutral or acid, liquids, pastes and solids, that can be analyzed by enzymatic methods. The oxidase - peroxidase system may be used for specific analyses. In the reaction catalyzed by the oxidase, hydrogen is transferred from the substrate to oxygen and hydrogen peroxide is formed as an intermediate. Hydrogen peroxide then reacts with a leuco dye in the presence of the enzyme peroxidase and a color develops which is measured in the visible spectrum. This article is a review of two enzymes, peroxidase (POD) and glycerol-3-phosphate oxidase (GPO), pointing out their principal sources, characteristics and applications.
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spelling doaj.art-1a910bf0e1db4c95901f23bea98404b52022-12-21T21:11:06ZengUniversidade Estadual PaulistaAlimentos e Nutrição0103-42352179-44482008-11-01181101109OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLSL. A. CAMARGOM. F. S. PERESE. A. L. GATTÁS<p class="MsoNormal" style="margin: 0cm 0cm 0pt; line-height: normal; text-align: justify; mso-layout-grid-align: none;"> Most well-known enzymatic methods have been employed in the bioanalysis of food, required by law in a number of countries. Food is a complex sample and hence difficult to analyze. In food analysis, there are many different types of sample, such as: clear or turbid, colorless or colored, neutral or acid, liquids, pastes and solids, that can be analyzed by enzymatic methods. The oxidase - peroxidase system may be used for specific analyses. In the reaction catalyzed by the oxidase, hydrogen is transferred from the substrate to oxygen and hydrogen peroxide is formed as an intermediate. Hydrogen peroxide then reacts with a leuco dye in the presence of the enzyme peroxidase and a color develops which is measured in the visible spectrum. This article is a review of two enzymes, peroxidase (POD) and glycerol-3-phosphate oxidase (GPO), pointing out their principal sources, characteristics and applications.http://serv-bib.fcfar.unesp.br/seer/index.php/alimentos/article/view/141Enzymesglycerol-3-phosphate oxidaseapplicationsperoxidaseapplications
spellingShingle L. A. CAMARGO
M. F. S. PERES
E. A. L. GATTÁS
OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS
Alimentos e Nutrição
Enzymes
glycerol-3-phosphate oxidase
applications
peroxidase
applications
title OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS
title_full OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS
title_fullStr OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS
title_full_unstemmed OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS
title_short OXIDOREDUCTASES FOR BIOANALYSIS AND FOOD CONTROLS
title_sort oxidoreductases for bioanalysis and food controls
topic Enzymes
glycerol-3-phosphate oxidase
applications
peroxidase
applications
url http://serv-bib.fcfar.unesp.br/seer/index.php/alimentos/article/view/141
work_keys_str_mv AT lacamargo oxidoreductasesforbioanalysisandfoodcontrols
AT mfsperes oxidoreductasesforbioanalysisandfoodcontrols
AT ealgattas oxidoreductasesforbioanalysisandfoodcontrols