Engineering Venom’s Toxin-Neutralizing Antibody Fragments and Its Therapeutic Potential
Serum therapy remains the only specific treatment against envenoming, but anti-venoms are still prepared by fragmentation of polyclonal antibodies isolated from hyper-immunized horse serum. Most of these anti-venoms are considered to be efficient, but their production is tedious, and their use may...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-08-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/6/8/2541 |
| _version_ | 1828153667896737792 |
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| author | Larissa M. Alvarenga Muhammad Zahid Anne di Tommaso Matthieu O. Juste Nicolas Aubrey Philippe Billiald Julien Muzard |
| author_facet | Larissa M. Alvarenga Muhammad Zahid Anne di Tommaso Matthieu O. Juste Nicolas Aubrey Philippe Billiald Julien Muzard |
| author_sort | Larissa M. Alvarenga |
| collection | DOAJ |
| description | Serum therapy remains the only specific treatment against envenoming, but anti-venoms are still prepared by fragmentation of polyclonal antibodies isolated from hyper-immunized horse serum. Most of these anti-venoms are considered to be efficient, but their production is tedious, and their use may be associated with adverse effects. Recombinant antibodies and smaller functional units are now emerging as credible alternatives and constitute a source of still unexploited biomolecules capable of neutralizing venoms. This review will be a walk through the technologies that have recently been applied leading to novel antibody formats with better properties in terms of homogeneity, specific activity and possible safety. |
| first_indexed | 2024-04-11T22:28:07Z |
| format | Article |
| id | doaj.art-1a91446c293548e9bd4a053c1858a43e |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T22:28:07Z |
| publishDate | 2014-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-1a91446c293548e9bd4a053c1858a43e2022-12-22T03:59:34ZengMDPI AGToxins2072-66512014-08-01682541256710.3390/toxins6082541toxins6082541Engineering Venom’s Toxin-Neutralizing Antibody Fragments and Its Therapeutic PotentialLarissa M. Alvarenga0Muhammad Zahid1Anne di Tommaso2Matthieu O. Juste3Nicolas Aubrey4Philippe Billiald5Julien Muzard6Muséum National d'Histoire Naturelle, UMR MNHN-CNRS 7245, 12 rue Buffon, 75231 Paris Cedex 05, FranceMuséum National d'Histoire Naturelle, UMR MNHN-CNRS 7245, 12 rue Buffon, 75231 Paris Cedex 05, FranceUniversité de Tours—INRA, UMR 1282, Faculté de Pharmacie, 31 Avenue Monge, 37200 Tours Cedex, FranceUniversité de Tours—INRA, UMR 1282, Faculté de Pharmacie, 31 Avenue Monge, 37200 Tours Cedex, FranceUniversité de Tours—INRA, UMR 1282, Faculté de Pharmacie, 31 Avenue Monge, 37200 Tours Cedex, FranceMuséum National d'Histoire Naturelle, UMR MNHN-CNRS 7245, 12 rue Buffon, 75231 Paris Cedex 05, FranceMuséum National d'Histoire Naturelle, UMR MNHN-CNRS 7245, 12 rue Buffon, 75231 Paris Cedex 05, FranceSerum therapy remains the only specific treatment against envenoming, but anti-venoms are still prepared by fragmentation of polyclonal antibodies isolated from hyper-immunized horse serum. Most of these anti-venoms are considered to be efficient, but their production is tedious, and their use may be associated with adverse effects. Recombinant antibodies and smaller functional units are now emerging as credible alternatives and constitute a source of still unexploited biomolecules capable of neutralizing venoms. This review will be a walk through the technologies that have recently been applied leading to novel antibody formats with better properties in terms of homogeneity, specific activity and possible safety.http://www.mdpi.com/2072-6651/6/8/2541scorpion stingssnakebiteantivenomserum therapyantibodyscFvdiabodynanobody |
| spellingShingle | Larissa M. Alvarenga Muhammad Zahid Anne di Tommaso Matthieu O. Juste Nicolas Aubrey Philippe Billiald Julien Muzard Engineering Venom’s Toxin-Neutralizing Antibody Fragments and Its Therapeutic Potential Toxins scorpion stings snakebite antivenom serum therapy antibody scFv diabody nanobody |
| title | Engineering Venom’s Toxin-Neutralizing Antibody Fragments and Its Therapeutic Potential |
| title_full | Engineering Venom’s Toxin-Neutralizing Antibody Fragments and Its Therapeutic Potential |
| title_fullStr | Engineering Venom’s Toxin-Neutralizing Antibody Fragments and Its Therapeutic Potential |
| title_full_unstemmed | Engineering Venom’s Toxin-Neutralizing Antibody Fragments and Its Therapeutic Potential |
| title_short | Engineering Venom’s Toxin-Neutralizing Antibody Fragments and Its Therapeutic Potential |
| title_sort | engineering venom s toxin neutralizing antibody fragments and its therapeutic potential |
| topic | scorpion stings snakebite antivenom serum therapy antibody scFv diabody nanobody |
| url | http://www.mdpi.com/2072-6651/6/8/2541 |
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