Effect of ACADL on the differentiation of goat subcutaneous adipocyte
Objective The aim of this study was to clone the mRNA sequence of the Acyl-CoA dehydrogenase long chain (ACADL) gene of goats and explore the effect of ACADL on the differentiation of subcutaneous fat cells on this basis. Methods We obtained the ACADL gene of goats by cloning and used quantitative r...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2023-06-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-22-0308.pdf |
| _version_ | 1797831731651280896 |
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| author | A Li YY Li QB Wuqie X Li H Zhang Y Wang YL Wang JJ Zhu YQ Lin |
| author_facet | A Li YY Li QB Wuqie X Li H Zhang Y Wang YL Wang JJ Zhu YQ Lin |
| author_sort | A Li |
| collection | DOAJ |
| description | Objective The aim of this study was to clone the mRNA sequence of the Acyl-CoA dehydrogenase long chain (ACADL) gene of goats and explore the effect of ACADL on the differentiation of subcutaneous fat cells on this basis. Methods We obtained the ACADL gene of goats by cloning and used quantitative real-time polymerase chain reaction (qPCR) to detect the ACADL expression patterns of different goat tissues and subcutaneous fat cells at different lipid induction stages. In addition, we transfect intramuscular and subcutaneous adipocytes separately by constructing overexpressed ACADL vectors and synthesizing Si-ACADL; finally, we observed the changes in oil red stained cell levels under the microscope, and qPCR detected changes in mRNA levels. Results The results showed goat ACADL gene expressed in sebum fat. During adipocyte differentiation, ACADL gradually increased from 0 to 24 h of culture, and decreased. Overexpression of ACADL promoted differentiation of subcutaneous adipocytes in goat and inhibited their differentiation after interference. Conclusion So, we infer ACADL may have an important role in positive regulating the differentiation process in goat subcutaneous adipocytes. This study will provide basic data for further study of the role of ACADL in goat subcutaneous adipocyte differentiation and lays the foundation for final elucidating of its molecular mechanisms in regulating subcutaneous fat deposition in goats. |
| first_indexed | 2024-04-09T13:57:29Z |
| format | Article |
| id | doaj.art-1a929b328884412ea099c06339290b98 |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-04-09T13:57:29Z |
| publishDate | 2023-06-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-1a929b328884412ea099c06339290b982023-05-07T23:25:09ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352023-06-0136682983910.5713/ab.22.030824998Effect of ACADL on the differentiation of goat subcutaneous adipocyteA Li0YY Li1QB Wuqie2X Li3H Zhang4Y Wang5YL Wang6JJ Zhu7YQ Lin8 Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu 610041, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu 610041, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu 610041, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu 610041, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu 610041, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu 610041, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu 610041, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu 610041, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu 610041, ChinaObjective The aim of this study was to clone the mRNA sequence of the Acyl-CoA dehydrogenase long chain (ACADL) gene of goats and explore the effect of ACADL on the differentiation of subcutaneous fat cells on this basis. Methods We obtained the ACADL gene of goats by cloning and used quantitative real-time polymerase chain reaction (qPCR) to detect the ACADL expression patterns of different goat tissues and subcutaneous fat cells at different lipid induction stages. In addition, we transfect intramuscular and subcutaneous adipocytes separately by constructing overexpressed ACADL vectors and synthesizing Si-ACADL; finally, we observed the changes in oil red stained cell levels under the microscope, and qPCR detected changes in mRNA levels. Results The results showed goat ACADL gene expressed in sebum fat. During adipocyte differentiation, ACADL gradually increased from 0 to 24 h of culture, and decreased. Overexpression of ACADL promoted differentiation of subcutaneous adipocytes in goat and inhibited their differentiation after interference. Conclusion So, we infer ACADL may have an important role in positive regulating the differentiation process in goat subcutaneous adipocytes. This study will provide basic data for further study of the role of ACADL in goat subcutaneous adipocyte differentiation and lays the foundation for final elucidating of its molecular mechanisms in regulating subcutaneous fat deposition in goats.http://www.animbiosci.org/upload/pdf/ab-22-0308.pdfacyl-coa dehydrogenase long chain (acadl)adipocytesdifferentiationgoatsubcutaneous |
| spellingShingle | A Li YY Li QB Wuqie X Li H Zhang Y Wang YL Wang JJ Zhu YQ Lin Effect of ACADL on the differentiation of goat subcutaneous adipocyte Animal Bioscience acyl-coa dehydrogenase long chain (acadl) adipocytes differentiation goat subcutaneous |
| title | Effect of ACADL on the differentiation of goat subcutaneous adipocyte |
| title_full | Effect of ACADL on the differentiation of goat subcutaneous adipocyte |
| title_fullStr | Effect of ACADL on the differentiation of goat subcutaneous adipocyte |
| title_full_unstemmed | Effect of ACADL on the differentiation of goat subcutaneous adipocyte |
| title_short | Effect of ACADL on the differentiation of goat subcutaneous adipocyte |
| title_sort | effect of acadl on the differentiation of goat subcutaneous adipocyte |
| topic | acyl-coa dehydrogenase long chain (acadl) adipocytes differentiation goat subcutaneous |
| url | http://www.animbiosci.org/upload/pdf/ab-22-0308.pdf |
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