Preliminary characterization of the self-incompatibility genotypes of European plum (Prunus domestica L.) cultivars

European plum is an important fruit crop with complex, hexaploid genome of unknown origin. The characterization of the selfincompatibility (S) locus of 16 European plum cultivars was carried out using the PaConsI-F primer in combination with the EM-PC1consRD primer for the first intron and the EM-PC...

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Main Authors: J. Halász, A. Kurilla, A. Hegedűs
Format: Article
Language:English
Published: University of Debrecen 2014-09-01
Series:International Journal of Horticultural Science
Subjects:
Online Access:https://ojs.lib.unideb.hu/IJHS/article/view/1128
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author J. Halász
A. Kurilla
A. Hegedűs
author_facet J. Halász
A. Kurilla
A. Hegedűs
author_sort J. Halász
collection DOAJ
description European plum is an important fruit crop with complex, hexaploid genome of unknown origin. The characterization of the selfincompatibility (S) locus of 16 European plum cultivars was carried out using the PaConsI-F primer in combination with the EM-PC1consRD primer for the first intron and the EM-PC2consFD and EM-PC3consRD primers for the second intron amplification. Altogether, 18 different alleles were scored indicating high genetic diversity. These alleles were labelled using alphabetical codes from SA to SS. We  identified 5 different alleles in 9 cultivars, 4 alleles in 5 cultivars, while 3 alleles were shown in two of the assayed cultivars. A total of 16 different S-genotypes were assigned, and discrimination of all plum cultivars was successful based on their unique S-genotypes. However, further research is required to reliably identify the S-alleles based on their DNA sequence and clarify complete S-genotypes.
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spelling doaj.art-1a96c02b2a1c4b59b369657b3d0944732022-12-21T22:10:11ZengUniversity of DebrecenInternational Journal of Horticultural Science1585-04042676-931X2014-09-01203-410.31421/IJHS/20/3-4/1128Preliminary characterization of the self-incompatibility genotypes of European plum (Prunus domestica L.) cultivarsJ. Halász0A. Kurilla1A. Hegedűs2Corvinus University of Budapest, Faculty of Horticultural Science, Department of Genetics and Plant Breeding, 1118 Budapest, Ménesi út 44., HungaryCorvinus University of Budapest, Faculty of Horticultural Science, Department of Genetics and Plant Breeding, 1118 Budapest, Ménesi út 44., HungaryCorvinus University of Budapest, Faculty of Horticultural Science, Department of Genetics and Plant Breeding, 1118 Budapest, Ménesi út 44., HungaryEuropean plum is an important fruit crop with complex, hexaploid genome of unknown origin. The characterization of the selfincompatibility (S) locus of 16 European plum cultivars was carried out using the PaConsI-F primer in combination with the EM-PC1consRD primer for the first intron and the EM-PC2consFD and EM-PC3consRD primers for the second intron amplification. Altogether, 18 different alleles were scored indicating high genetic diversity. These alleles were labelled using alphabetical codes from SA to SS. We  identified 5 different alleles in 9 cultivars, 4 alleles in 5 cultivars, while 3 alleles were shown in two of the assayed cultivars. A total of 16 different S-genotypes were assigned, and discrimination of all plum cultivars was successful based on their unique S-genotypes. However, further research is required to reliably identify the S-alleles based on their DNA sequence and clarify complete S-genotypes.https://ojs.lib.unideb.hu/IJHS/article/view/1128self-incompatibilityS-alleleEuropean plumPrunus domestica L.
spellingShingle J. Halász
A. Kurilla
A. Hegedűs
Preliminary characterization of the self-incompatibility genotypes of European plum (Prunus domestica L.) cultivars
International Journal of Horticultural Science
self-incompatibility
S-allele
European plum
Prunus domestica L.
title Preliminary characterization of the self-incompatibility genotypes of European plum (Prunus domestica L.) cultivars
title_full Preliminary characterization of the self-incompatibility genotypes of European plum (Prunus domestica L.) cultivars
title_fullStr Preliminary characterization of the self-incompatibility genotypes of European plum (Prunus domestica L.) cultivars
title_full_unstemmed Preliminary characterization of the self-incompatibility genotypes of European plum (Prunus domestica L.) cultivars
title_short Preliminary characterization of the self-incompatibility genotypes of European plum (Prunus domestica L.) cultivars
title_sort preliminary characterization of the self incompatibility genotypes of european plum prunus domestica l cultivars
topic self-incompatibility
S-allele
European plum
Prunus domestica L.
url https://ojs.lib.unideb.hu/IJHS/article/view/1128
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AT akurilla preliminarycharacterizationoftheselfincompatibilitygenotypesofeuropeanplumprunusdomesticalcultivars
AT ahegedus preliminarycharacterizationoftheselfincompatibilitygenotypesofeuropeanplumprunusdomesticalcultivars