Anthelmintic Activity of <i>Tanacetum vulgare</i> L. (Leaf and Flower) Extracts against Trichostrongylidae Nematodes in Sheep In Vitro
Due to the high prevalence of gastrointestinal nematodes in sheep, the growing anthelmintic resistance, and the development of organic farming systems, sustainable alternatives are being sought. One such method is phytotherapy. This study aimed to evaluate the in vitro ovicidal and larvicidal activi...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/13/2176 |
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| author | Alīna Kļaviņa Dace Keidāne Kristīne Ganola Ivars Lūsis Renāte Šukele Dace Bandere Liga Kovalcuka |
| author_facet | Alīna Kļaviņa Dace Keidāne Kristīne Ganola Ivars Lūsis Renāte Šukele Dace Bandere Liga Kovalcuka |
| author_sort | Alīna Kļaviņa |
| collection | DOAJ |
| description | Due to the high prevalence of gastrointestinal nematodes in sheep, the growing anthelmintic resistance, and the development of organic farming systems, sustainable alternatives are being sought. One such method is phytotherapy. This study aimed to evaluate the in vitro ovicidal and larvicidal activity of extracts of tansy (<i>Tanacetum vulgare</i> L.) growing in Latvia on gastrointestinal nematodes (Trichostrongylidae) in sheep. The leaves and flowers of the tansy were extracted separately in 70%, 50%, and 30% ethanol and acetone. Six concentrations were prepared from each extract 500 mg/mL, 200 mg/mL, 100 mg/mL, 50 mg/mL, 20 mg/mL, and 10 mg/mL. In vitro egg hatching test and micro-agar larval development test were performed. Extracts of tansy have strong larvicidal activity. The highest percentage of larvae inhibition for most of the extracts was 100%, but for egg inhibition, it was 95.8% for the 200 mg/mL concentration of 50% acetone and 93.3% for the 500 mg/mL concentration of 50% ethanol leaf extracts. All tansy extracts had ovicidal and larvicidal activity against Trichostrongylidae in sheep. |
| first_indexed | 2024-03-11T01:47:46Z |
| format | Article |
| id | doaj.art-1a98f1e6ba614c8ea5874c7fd34b08f8 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T01:47:46Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-1a98f1e6ba614c8ea5874c7fd34b08f82023-11-18T16:04:06ZengMDPI AGAnimals2076-26152023-07-011313217610.3390/ani13132176Anthelmintic Activity of <i>Tanacetum vulgare</i> L. (Leaf and Flower) Extracts against Trichostrongylidae Nematodes in Sheep In VitroAlīna Kļaviņa0Dace Keidāne1Kristīne Ganola2Ivars Lūsis3Renāte Šukele4Dace Bandere5Liga Kovalcuka6Institute of Food and Environmental Hygiene, Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, K. Helmana Street 8, LV-3004 Jelgava, LatviaInstitute of Food and Environmental Hygiene, Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, K. Helmana Street 8, LV-3004 Jelgava, LatviaInstitute of Food and Environmental Hygiene, Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, K. Helmana Street 8, LV-3004 Jelgava, LatviaInstitute of Food and Environmental Hygiene, Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, K. Helmana Street 8, LV-3004 Jelgava, LatviaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Rīga Stradiņš University, Konsula Street 21, LV-1007 Riga, LatviaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Rīga Stradiņš University, Konsula Street 21, LV-1007 Riga, LatviaClinical Institute, Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, K. Helmana Street 8, LV-3004 Jelgava, LatviaDue to the high prevalence of gastrointestinal nematodes in sheep, the growing anthelmintic resistance, and the development of organic farming systems, sustainable alternatives are being sought. One such method is phytotherapy. This study aimed to evaluate the in vitro ovicidal and larvicidal activity of extracts of tansy (<i>Tanacetum vulgare</i> L.) growing in Latvia on gastrointestinal nematodes (Trichostrongylidae) in sheep. The leaves and flowers of the tansy were extracted separately in 70%, 50%, and 30% ethanol and acetone. Six concentrations were prepared from each extract 500 mg/mL, 200 mg/mL, 100 mg/mL, 50 mg/mL, 20 mg/mL, and 10 mg/mL. In vitro egg hatching test and micro-agar larval development test were performed. Extracts of tansy have strong larvicidal activity. The highest percentage of larvae inhibition for most of the extracts was 100%, but for egg inhibition, it was 95.8% for the 200 mg/mL concentration of 50% acetone and 93.3% for the 500 mg/mL concentration of 50% ethanol leaf extracts. All tansy extracts had ovicidal and larvicidal activity against Trichostrongylidae in sheep.https://www.mdpi.com/2076-2615/13/13/2176phytotherapyplant extracts<i>Tanacetum vulgare</i>sheepgastrointestinal parasitesanthelmintic resistance |
| spellingShingle | Alīna Kļaviņa Dace Keidāne Kristīne Ganola Ivars Lūsis Renāte Šukele Dace Bandere Liga Kovalcuka Anthelmintic Activity of <i>Tanacetum vulgare</i> L. (Leaf and Flower) Extracts against Trichostrongylidae Nematodes in Sheep In Vitro Animals phytotherapy plant extracts <i>Tanacetum vulgare</i> sheep gastrointestinal parasites anthelmintic resistance |
| title | Anthelmintic Activity of <i>Tanacetum vulgare</i> L. (Leaf and Flower) Extracts against Trichostrongylidae Nematodes in Sheep In Vitro |
| title_full | Anthelmintic Activity of <i>Tanacetum vulgare</i> L. (Leaf and Flower) Extracts against Trichostrongylidae Nematodes in Sheep In Vitro |
| title_fullStr | Anthelmintic Activity of <i>Tanacetum vulgare</i> L. (Leaf and Flower) Extracts against Trichostrongylidae Nematodes in Sheep In Vitro |
| title_full_unstemmed | Anthelmintic Activity of <i>Tanacetum vulgare</i> L. (Leaf and Flower) Extracts against Trichostrongylidae Nematodes in Sheep In Vitro |
| title_short | Anthelmintic Activity of <i>Tanacetum vulgare</i> L. (Leaf and Flower) Extracts against Trichostrongylidae Nematodes in Sheep In Vitro |
| title_sort | anthelmintic activity of i tanacetum vulgare i l leaf and flower extracts against trichostrongylidae nematodes in sheep in vitro |
| topic | phytotherapy plant extracts <i>Tanacetum vulgare</i> sheep gastrointestinal parasites anthelmintic resistance |
| url | https://www.mdpi.com/2076-2615/13/13/2176 |
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