Establishment of repeated liver biopsy technique in experimental mice
Biopsy is a commonly used method for determining pathological diagnoses by directly using human tissues and cells. Biopsies are widely used to determine disease progression and treatment efficacy. Although organs and tissues are usually obtained by sacrifice during animal experiments, it is theoreti...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023041853 |
| _version_ | 1797807156382138368 |
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| author | Wenhua Shao Mayuko Ichimura-Shimizu Hirohisa Ogawa Shengjian Jin Mitsuko Sutoh Satoko Nakamura Miki Onodera Hirosuke Tawara Shunji Toyohara Ryoji Hokao Yasusei Kudo Takeshi Oya Koichi Tsuneyama |
| author_facet | Wenhua Shao Mayuko Ichimura-Shimizu Hirohisa Ogawa Shengjian Jin Mitsuko Sutoh Satoko Nakamura Miki Onodera Hirosuke Tawara Shunji Toyohara Ryoji Hokao Yasusei Kudo Takeshi Oya Koichi Tsuneyama |
| author_sort | Wenhua Shao |
| collection | DOAJ |
| description | Biopsy is a commonly used method for determining pathological diagnoses by directly using human tissues and cells. Biopsies are widely used to determine disease progression and treatment efficacy. Although organs and tissues are usually obtained by sacrifice during animal experiments, it is theoretically possible to use the same biopsy techniques in humans. In the present study, we examined the feasibility of performing four repeated liver biopsies in a spontaneous metabolic syndrome mouse model. Even though a small number of mice died accidently, most mice were able to undergo four liver biopsies without significant adverse events. We also performed three liver biopsies in mouse liver tumor carcinogen models at 4, 8, and 12 weeks of age. In addition to the sample collected at 16 weeks of age during sacrifice, we successfully collected four liver samples from the same mice at different stages of disease progression. The application of this liver biopsy technique might make it possible for direct evaluation of pathological conditions in the same individual over time, thereby reducing the number of experimental animals. |
| first_indexed | 2024-03-13T06:18:19Z |
| format | Article |
| id | doaj.art-1a99eb2914954b22a6fbef791738c239 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T06:18:19Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-1a99eb2914954b22a6fbef791738c2392023-06-10T04:28:14ZengElsevierHeliyon2405-84402023-06-0196e16978Establishment of repeated liver biopsy technique in experimental miceWenhua Shao0Mayuko Ichimura-Shimizu1Hirohisa Ogawa2Shengjian Jin3Mitsuko Sutoh4Satoko Nakamura5Miki Onodera6Hirosuke Tawara7Shunji Toyohara8Ryoji Hokao9Yasusei Kudo10Takeshi Oya11Koichi Tsuneyama12Department of Molecular Pathology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan; Department of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, Tokushima, JapanDepartment of Pathology and Laboratory Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Pathology and Laboratory Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, Tokushima, JapanInstitute for Animal Reproduction, Kasumigaura, Ibaraki, JapanInstitute for Animal Reproduction, Kasumigaura, Ibaraki, JapanInstitute for Animal Reproduction, Kasumigaura, Ibaraki, JapanInstitute for Animal Reproduction, Kasumigaura, Ibaraki, JapanInstitute for Animal Reproduction, Kasumigaura, Ibaraki, JapanInstitute for Animal Reproduction, Kasumigaura, Ibaraki, JapanDepartment of Oral Bioscience, Tokushima University Graduate School of Biomedical Sciences, Tokushima, JapanDepartment of Molecular Pathology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, JapanDepartment of Pathology and Laboratory Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan; Corresponding author. Department of Pathology and Laboratory Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan.Biopsy is a commonly used method for determining pathological diagnoses by directly using human tissues and cells. Biopsies are widely used to determine disease progression and treatment efficacy. Although organs and tissues are usually obtained by sacrifice during animal experiments, it is theoretically possible to use the same biopsy techniques in humans. In the present study, we examined the feasibility of performing four repeated liver biopsies in a spontaneous metabolic syndrome mouse model. Even though a small number of mice died accidently, most mice were able to undergo four liver biopsies without significant adverse events. We also performed three liver biopsies in mouse liver tumor carcinogen models at 4, 8, and 12 weeks of age. In addition to the sample collected at 16 weeks of age during sacrifice, we successfully collected four liver samples from the same mice at different stages of disease progression. The application of this liver biopsy technique might make it possible for direct evaluation of pathological conditions in the same individual over time, thereby reducing the number of experimental animals.http://www.sciencedirect.com/science/article/pii/S2405844023041853Liver biopsyMetabolic syndromeMouse model |
| spellingShingle | Wenhua Shao Mayuko Ichimura-Shimizu Hirohisa Ogawa Shengjian Jin Mitsuko Sutoh Satoko Nakamura Miki Onodera Hirosuke Tawara Shunji Toyohara Ryoji Hokao Yasusei Kudo Takeshi Oya Koichi Tsuneyama Establishment of repeated liver biopsy technique in experimental mice Heliyon Liver biopsy Metabolic syndrome Mouse model |
| title | Establishment of repeated liver biopsy technique in experimental mice |
| title_full | Establishment of repeated liver biopsy technique in experimental mice |
| title_fullStr | Establishment of repeated liver biopsy technique in experimental mice |
| title_full_unstemmed | Establishment of repeated liver biopsy technique in experimental mice |
| title_short | Establishment of repeated liver biopsy technique in experimental mice |
| title_sort | establishment of repeated liver biopsy technique in experimental mice |
| topic | Liver biopsy Metabolic syndrome Mouse model |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023041853 |
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