Improving Vesicular Integrity and Antioxidant Activity of Novel Mixed Soy Lecithin-Based Liposomes Containing Squalene and Their Stability against UV Light
In order to improve the membrane lipophilicity and the affinity towards the environment of lipid bilayers, squalene (SQ) could be conjugated to phospholipids in the formation of liposomes. The effect of membrane composition and concentrations on the degradation of liposomes prepared via the extrusio...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-12-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/25/24/5873 |
| _version_ | 1797544932614864896 |
|---|---|
| author | Sahar Pakbaten Toopkanloo Tai Boon Tan Faridah Abas Mohammad Azam Imededdine Arbi Nehdi Chin Ping Tan |
| author_facet | Sahar Pakbaten Toopkanloo Tai Boon Tan Faridah Abas Mohammad Azam Imededdine Arbi Nehdi Chin Ping Tan |
| author_sort | Sahar Pakbaten Toopkanloo |
| collection | DOAJ |
| description | In order to improve the membrane lipophilicity and the affinity towards the environment of lipid bilayers, squalene (SQ) could be conjugated to phospholipids in the formation of liposomes. The effect of membrane composition and concentrations on the degradation of liposomes prepared via the extrusion method was investigated. Liposomes were prepared using a mixture of SQ, cholesterol (CH) and Tween80 (TW80). Based on the optimal conditions, liposome batches were prepared in the absence and presence of SQ. Their physicochemical and stability behavior were evaluated as a function of liposome constituent. From the optimization study, the liposomal formulation containing 5% (<i>w</i>/<i>w</i>) mixed soy lecithin (ML), 0.5% (<i>w</i>/<i>w</i>) SQ, 0.3% (<i>w</i>/<i>w</i>) CH and 0.75% (<i>w</i>/<i>w</i>) TW80 had optimal physicochemical properties and displayed a unilamellar structure. Liposome prepared using the optimal formulation had a low particle size (158.31 ± 2.96 nm) and acceptable %increase in the particle size (15.09% ± 3.76%) and %trolox equivalent antioxidant capacity (%TEAC) loss (35.69% ± 0.72%) against UV light treatment (280–320 nm) for 6 h. The interesting outcome of this research was the association of naturally occurring substance SQ for size reduction without the extra input of energy or mechanical procedures, and improvement of vesicle stability and antioxidant activity of ML-based liposome. This study also demonstrated that the presence of SQ in the membrane might increase the acyl chain dynamics and decrease the viscosity of the dispersion, thereby limiting long-term stability of the liposome. |
| first_indexed | 2024-03-10T14:07:30Z |
| format | Article |
| id | doaj.art-1aa27c2cc9824ffd9b3b116ac6686b7f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:07:30Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1aa27c2cc9824ffd9b3b116ac6686b7f2023-11-21T00:27:53ZengMDPI AGMolecules1420-30492020-12-012524587310.3390/molecules25245873Improving Vesicular Integrity and Antioxidant Activity of Novel Mixed Soy Lecithin-Based Liposomes Containing Squalene and Their Stability against UV LightSahar Pakbaten Toopkanloo0Tai Boon Tan1Faridah Abas2Mohammad Azam3Imededdine Arbi Nehdi4Chin Ping Tan5Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, MalaysiaDepartment of Food Service and Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, MalaysiaChemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaChemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, MalaysiaIn order to improve the membrane lipophilicity and the affinity towards the environment of lipid bilayers, squalene (SQ) could be conjugated to phospholipids in the formation of liposomes. The effect of membrane composition and concentrations on the degradation of liposomes prepared via the extrusion method was investigated. Liposomes were prepared using a mixture of SQ, cholesterol (CH) and Tween80 (TW80). Based on the optimal conditions, liposome batches were prepared in the absence and presence of SQ. Their physicochemical and stability behavior were evaluated as a function of liposome constituent. From the optimization study, the liposomal formulation containing 5% (<i>w</i>/<i>w</i>) mixed soy lecithin (ML), 0.5% (<i>w</i>/<i>w</i>) SQ, 0.3% (<i>w</i>/<i>w</i>) CH and 0.75% (<i>w</i>/<i>w</i>) TW80 had optimal physicochemical properties and displayed a unilamellar structure. Liposome prepared using the optimal formulation had a low particle size (158.31 ± 2.96 nm) and acceptable %increase in the particle size (15.09% ± 3.76%) and %trolox equivalent antioxidant capacity (%TEAC) loss (35.69% ± 0.72%) against UV light treatment (280–320 nm) for 6 h. The interesting outcome of this research was the association of naturally occurring substance SQ for size reduction without the extra input of energy or mechanical procedures, and improvement of vesicle stability and antioxidant activity of ML-based liposome. This study also demonstrated that the presence of SQ in the membrane might increase the acyl chain dynamics and decrease the viscosity of the dispersion, thereby limiting long-term stability of the liposome.https://www.mdpi.com/1420-3049/25/24/5873liposome integritysqualenemembrane compositionphotodegradationX-ray diffraction |
| spellingShingle | Sahar Pakbaten Toopkanloo Tai Boon Tan Faridah Abas Mohammad Azam Imededdine Arbi Nehdi Chin Ping Tan Improving Vesicular Integrity and Antioxidant Activity of Novel Mixed Soy Lecithin-Based Liposomes Containing Squalene and Their Stability against UV Light Molecules liposome integrity squalene membrane composition photodegradation X-ray diffraction |
| title | Improving Vesicular Integrity and Antioxidant Activity of Novel Mixed Soy Lecithin-Based Liposomes Containing Squalene and Their Stability against UV Light |
| title_full | Improving Vesicular Integrity and Antioxidant Activity of Novel Mixed Soy Lecithin-Based Liposomes Containing Squalene and Their Stability against UV Light |
| title_fullStr | Improving Vesicular Integrity and Antioxidant Activity of Novel Mixed Soy Lecithin-Based Liposomes Containing Squalene and Their Stability against UV Light |
| title_full_unstemmed | Improving Vesicular Integrity and Antioxidant Activity of Novel Mixed Soy Lecithin-Based Liposomes Containing Squalene and Their Stability against UV Light |
| title_short | Improving Vesicular Integrity and Antioxidant Activity of Novel Mixed Soy Lecithin-Based Liposomes Containing Squalene and Their Stability against UV Light |
| title_sort | improving vesicular integrity and antioxidant activity of novel mixed soy lecithin based liposomes containing squalene and their stability against uv light |
| topic | liposome integrity squalene membrane composition photodegradation X-ray diffraction |
| url | https://www.mdpi.com/1420-3049/25/24/5873 |
| work_keys_str_mv | AT saharpakbatentoopkanloo improvingvesicularintegrityandantioxidantactivityofnovelmixedsoylecithinbasedliposomescontainingsqualeneandtheirstabilityagainstuvlight AT taiboontan improvingvesicularintegrityandantioxidantactivityofnovelmixedsoylecithinbasedliposomescontainingsqualeneandtheirstabilityagainstuvlight AT faridahabas improvingvesicularintegrityandantioxidantactivityofnovelmixedsoylecithinbasedliposomescontainingsqualeneandtheirstabilityagainstuvlight AT mohammadazam improvingvesicularintegrityandantioxidantactivityofnovelmixedsoylecithinbasedliposomescontainingsqualeneandtheirstabilityagainstuvlight AT imededdinearbinehdi improvingvesicularintegrityandantioxidantactivityofnovelmixedsoylecithinbasedliposomescontainingsqualeneandtheirstabilityagainstuvlight AT chinpingtan improvingvesicularintegrityandantioxidantactivityofnovelmixedsoylecithinbasedliposomescontainingsqualeneandtheirstabilityagainstuvlight |