Correlation of the tumor escape phenotype with loss of PRELP expression in melanoma
Abstract Background Despite immunotherapies having revolutionized the treatment of advanced cutaneous melanoma, effective and durable responses were only reported in a few patients. A better understanding of the interaction of melanoma cells with the microenvironment, including extracellular matrix...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12967-023-04476-x |
| _version_ | 1797556827183906816 |
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| author | Helene Schäfer Karthikeyan Subbarayan Chiara Massa Christoforos Vaxevanis Anja Mueller Barbara Seliger |
| author_facet | Helene Schäfer Karthikeyan Subbarayan Chiara Massa Christoforos Vaxevanis Anja Mueller Barbara Seliger |
| author_sort | Helene Schäfer |
| collection | DOAJ |
| description | Abstract Background Despite immunotherapies having revolutionized the treatment of advanced cutaneous melanoma, effective and durable responses were only reported in a few patients. A better understanding of the interaction of melanoma cells with the microenvironment, including extracellular matrix (ECM) components, might provide novel therapeutic options. Although the ECM has been linked to several hallmarks of cancer, little information is available regarding the expression and function of the ECM protein purine-arginine-rich and leucine-rich protein (PRELP) in cancer, including melanoma. Methods The structural integrity, expression and function of PRELP, its correlation with the expression of immune modulatory molecules, immune cell infiltration and clinical parameters were determined using standard methods and/or bioinformatics. Results Bioinformatics analysis revealed a heterogeneous, but statistically significant reduced PRELP expression in available datasets of skin cutaneous melanoma when compared to adjacent normal tissues, which was associated with reduced patients’ survival, low expression levels of components of the MHC class I antigen processing machinery (APM) and interferon (IFN)-γ signal transduction pathway, but increased expression of the transforming growth factor (TGF)-β isoform 1 (TFGB1) and TGF-β receptor 1 (TGFBR1). In addition, a high frequency of intra-tumoral T cells directly correlated with the expression of MHC class I and PRELP as well as the T cell attractant CCL5 in melanoma lesions. Marginal to low PRELP expression levels were found in the 47/49 human melanoma cell lines analysis. Transfection of PRELP into melanoma cell lines restored MHC class I surface expression due to transcriptional upregulation of major MHC class I APM and IFN-γ pathway components. In addition, PRELP overexpression is accompanied by high CCL5 secretion levels in cell supernatant, an impaired TGF-β signaling as well as a reduced cell proliferation, migration and invasion of melanoma cells. Conclusions Our findings suggest that PRELP induces the expression of MHC class I and CCL5 in melanoma, which might be involved in an enhanced T cell recruitment and immunogenicity associated with an improved patients’ outcome. Therefore, PRELP might serve as a marker for predicting disease progression and its recovery could revert the tumorigenic phenotype, which represents a novel therapeutic option for melanoma. |
| first_indexed | 2024-03-10T17:08:30Z |
| format | Article |
| id | doaj.art-1aa529272d1a421987567c94e5b26a5f |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-03-10T17:08:30Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-1aa529272d1a421987567c94e5b26a5f2023-11-20T10:43:45ZengBMCJournal of Translational Medicine1479-58762023-09-0121111610.1186/s12967-023-04476-xCorrelation of the tumor escape phenotype with loss of PRELP expression in melanomaHelene Schäfer0Karthikeyan Subbarayan1Chiara Massa2Christoforos Vaxevanis3Anja Mueller4Barbara Seliger5Medical Faculty, Martin Luther University Halle-WittenbergMedical Faculty, Martin Luther University Halle-WittenbergMedical Faculty, Martin Luther University Halle-WittenbergMedical Faculty, Martin Luther University Halle-WittenbergMedical Faculty, Martin Luther University Halle-WittenbergMedical Faculty, Martin Luther University Halle-WittenbergAbstract Background Despite immunotherapies having revolutionized the treatment of advanced cutaneous melanoma, effective and durable responses were only reported in a few patients. A better understanding of the interaction of melanoma cells with the microenvironment, including extracellular matrix (ECM) components, might provide novel therapeutic options. Although the ECM has been linked to several hallmarks of cancer, little information is available regarding the expression and function of the ECM protein purine-arginine-rich and leucine-rich protein (PRELP) in cancer, including melanoma. Methods The structural integrity, expression and function of PRELP, its correlation with the expression of immune modulatory molecules, immune cell infiltration and clinical parameters were determined using standard methods and/or bioinformatics. Results Bioinformatics analysis revealed a heterogeneous, but statistically significant reduced PRELP expression in available datasets of skin cutaneous melanoma when compared to adjacent normal tissues, which was associated with reduced patients’ survival, low expression levels of components of the MHC class I antigen processing machinery (APM) and interferon (IFN)-γ signal transduction pathway, but increased expression of the transforming growth factor (TGF)-β isoform 1 (TFGB1) and TGF-β receptor 1 (TGFBR1). In addition, a high frequency of intra-tumoral T cells directly correlated with the expression of MHC class I and PRELP as well as the T cell attractant CCL5 in melanoma lesions. Marginal to low PRELP expression levels were found in the 47/49 human melanoma cell lines analysis. Transfection of PRELP into melanoma cell lines restored MHC class I surface expression due to transcriptional upregulation of major MHC class I APM and IFN-γ pathway components. In addition, PRELP overexpression is accompanied by high CCL5 secretion levels in cell supernatant, an impaired TGF-β signaling as well as a reduced cell proliferation, migration and invasion of melanoma cells. Conclusions Our findings suggest that PRELP induces the expression of MHC class I and CCL5 in melanoma, which might be involved in an enhanced T cell recruitment and immunogenicity associated with an improved patients’ outcome. Therefore, PRELP might serve as a marker for predicting disease progression and its recovery could revert the tumorigenic phenotype, which represents a novel therapeutic option for melanoma.https://doi.org/10.1186/s12967-023-04476-xPRELPMelanomaMHC class IAntigen processingImmunogenicityIFN signaling |
| spellingShingle | Helene Schäfer Karthikeyan Subbarayan Chiara Massa Christoforos Vaxevanis Anja Mueller Barbara Seliger Correlation of the tumor escape phenotype with loss of PRELP expression in melanoma Journal of Translational Medicine PRELP Melanoma MHC class I Antigen processing Immunogenicity IFN signaling |
| title | Correlation of the tumor escape phenotype with loss of PRELP expression in melanoma |
| title_full | Correlation of the tumor escape phenotype with loss of PRELP expression in melanoma |
| title_fullStr | Correlation of the tumor escape phenotype with loss of PRELP expression in melanoma |
| title_full_unstemmed | Correlation of the tumor escape phenotype with loss of PRELP expression in melanoma |
| title_short | Correlation of the tumor escape phenotype with loss of PRELP expression in melanoma |
| title_sort | correlation of the tumor escape phenotype with loss of prelp expression in melanoma |
| topic | PRELP Melanoma MHC class I Antigen processing Immunogenicity IFN signaling |
| url | https://doi.org/10.1186/s12967-023-04476-x |
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