ISSR-Based Genetic Diversity Assessment of Genus <i>J</i><i>asminum</i> L. (Oleaceae) from Pakistan
The genus <i>Jasminum</i> L., of the family Oleaceae, includes many species occurring in the wild, or cultivated worldwide. A preliminary investigation based on inter-simple sequence repeats (ISSR) was performed to assess the genetic diversity among 28 accessions, representing nine speci...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/2223-7747/10/7/1270 |
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| author | Naeem Akhtar Ishfaq Ahmad Hafiz Muhammad Qasim Hayat Daniel Potter Nadeem Akhtar Abbasi Umer Habib Adil Hussain Hina Hafeez Muhammad Ajmal Bashir Saad Imran Malik |
| author_facet | Naeem Akhtar Ishfaq Ahmad Hafiz Muhammad Qasim Hayat Daniel Potter Nadeem Akhtar Abbasi Umer Habib Adil Hussain Hina Hafeez Muhammad Ajmal Bashir Saad Imran Malik |
| author_sort | Naeem Akhtar |
| collection | DOAJ |
| description | The genus <i>Jasminum</i> L., of the family Oleaceae, includes many species occurring in the wild, or cultivated worldwide. A preliminary investigation based on inter-simple sequence repeats (ISSR) was performed to assess the genetic diversity among 28 accessions, representing nine species of <i>Jasminum</i> from various regions, representing a range of altitudes in Pakistan. A total of 21 ISSR primers were used, which produced 570 amplified bands of different sizes, with a mean polymorphic band percentage of 98.26%. The maximum resolving power, polymorphism information content, and index values of the ISSR markers recorded for primers 6, 16, and 19 were 0.40, 12.32, and 24.21, respectively. Based on the data of the ISSR markers, the resulting UPGMA dendrogram with the Jaccard coefficient divided the 28 accessions into two main clades. At the species level, the highest values for Shannon’s information index, polymorphism percentage, effective allele number, Nei’s genetic variations, and genetic unbiased diversity were found in <i>Jasminum sambac</i> L. and <i>J. humile</i> L., while the lowest were observed in <i>J. mesnyi</i> Hance and <i>J. nitidum</i> Skan. Based on Nei’s unbiased genetic identity pairwise population matrix, the maximum identity (0.804) was observed between <i>J. elongatum</i> Willd and <i>J. multiflorum</i> (Burm. f.) Andrews, and the lowest (0.566) between <i>J. nitidum</i> Skan. and <i>J. azoricum</i> L. Molecular variance analysis displayed a genetic variation of 79% among the nine populations. The study was aimed to established genetic diversity in <i>Jasminum</i> species using ISSR markers. With the help of this technique, we were able to establish immense intra- and interspecific diversity across the <i>Jasminum</i> species. |
| first_indexed | 2024-03-10T10:09:25Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
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| publishDate | 2021-06-01 |
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| spelling | doaj.art-1aa7f12d9c1c44458f3431634b5d9fd42023-11-22T01:16:02ZengMDPI AGPlants2223-77472021-06-01107127010.3390/plants10071270ISSR-Based Genetic Diversity Assessment of Genus <i>J</i><i>asminum</i> L. (Oleaceae) from PakistanNaeem Akhtar0Ishfaq Ahmad Hafiz1Muhammad Qasim Hayat2Daniel Potter3Nadeem Akhtar Abbasi4Umer Habib5Adil Hussain6Hina Hafeez7Muhammad Ajmal Bashir8Saad Imran Malik9Department of Horticulture, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46000, PakistanDepartment of Horticulture, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46000, PakistanPlant Systematics and Evolution Laboratory, Department of Plant Biotechnology, Atta ur Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Islamabad 44000, PakistanDepartment of Plant Sciences, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616, USADepartment of Horticulture, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46000, PakistanDepartment of Horticulture, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46000, PakistanDepartment of Biotechnology, Faculty of Life Sciences, University of Okara, Okara 56130, PakistanNational Institute for Genomics and Advanced Biotechnology, National Agriculture Research Center, Islamabad 44000, PakistanDepartment of Horticulture, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46000, PakistanDepartment of Plant Breeding and Genetics, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46000, PakistanThe genus <i>Jasminum</i> L., of the family Oleaceae, includes many species occurring in the wild, or cultivated worldwide. A preliminary investigation based on inter-simple sequence repeats (ISSR) was performed to assess the genetic diversity among 28 accessions, representing nine species of <i>Jasminum</i> from various regions, representing a range of altitudes in Pakistan. A total of 21 ISSR primers were used, which produced 570 amplified bands of different sizes, with a mean polymorphic band percentage of 98.26%. The maximum resolving power, polymorphism information content, and index values of the ISSR markers recorded for primers 6, 16, and 19 were 0.40, 12.32, and 24.21, respectively. Based on the data of the ISSR markers, the resulting UPGMA dendrogram with the Jaccard coefficient divided the 28 accessions into two main clades. At the species level, the highest values for Shannon’s information index, polymorphism percentage, effective allele number, Nei’s genetic variations, and genetic unbiased diversity were found in <i>Jasminum sambac</i> L. and <i>J. humile</i> L., while the lowest were observed in <i>J. mesnyi</i> Hance and <i>J. nitidum</i> Skan. Based on Nei’s unbiased genetic identity pairwise population matrix, the maximum identity (0.804) was observed between <i>J. elongatum</i> Willd and <i>J. multiflorum</i> (Burm. f.) Andrews, and the lowest (0.566) between <i>J. nitidum</i> Skan. and <i>J. azoricum</i> L. Molecular variance analysis displayed a genetic variation of 79% among the nine populations. The study was aimed to established genetic diversity in <i>Jasminum</i> species using ISSR markers. With the help of this technique, we were able to establish immense intra- and interspecific diversity across the <i>Jasminum</i> species.https://www.mdpi.com/2223-7747/10/7/1270<i>Jasminum</i> L.genetic diversityISSRPakistanpolymorphism |
| spellingShingle | Naeem Akhtar Ishfaq Ahmad Hafiz Muhammad Qasim Hayat Daniel Potter Nadeem Akhtar Abbasi Umer Habib Adil Hussain Hina Hafeez Muhammad Ajmal Bashir Saad Imran Malik ISSR-Based Genetic Diversity Assessment of Genus <i>J</i><i>asminum</i> L. (Oleaceae) from Pakistan Plants <i>Jasminum</i> L. genetic diversity ISSR Pakistan polymorphism |
| title | ISSR-Based Genetic Diversity Assessment of Genus <i>J</i><i>asminum</i> L. (Oleaceae) from Pakistan |
| title_full | ISSR-Based Genetic Diversity Assessment of Genus <i>J</i><i>asminum</i> L. (Oleaceae) from Pakistan |
| title_fullStr | ISSR-Based Genetic Diversity Assessment of Genus <i>J</i><i>asminum</i> L. (Oleaceae) from Pakistan |
| title_full_unstemmed | ISSR-Based Genetic Diversity Assessment of Genus <i>J</i><i>asminum</i> L. (Oleaceae) from Pakistan |
| title_short | ISSR-Based Genetic Diversity Assessment of Genus <i>J</i><i>asminum</i> L. (Oleaceae) from Pakistan |
| title_sort | issr based genetic diversity assessment of genus i j i i asminum i l oleaceae from pakistan |
| topic | <i>Jasminum</i> L. genetic diversity ISSR Pakistan polymorphism |
| url | https://www.mdpi.com/2223-7747/10/7/1270 |
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