Bio-Based Decontamination and Detoxification of Total Petroleum Hydrocarbon-Contaminated Dredged Sediments: Perspectives to Produce Constructed Technosols in the Frame of the Circular Economy
To accelerate the depletion of total petroleum hydrocarbons, a hydrocarburoclastic ascomycetes, <i>Lambertella</i> sp. MUT 5852, was bioaugmented to dredged sediments co-composting with a lignocellulosic matrix. After only 28 days of incubation, a complete depletion of the contamination...
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MDPI AG
2023-11-01
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| Online Access: | https://www.mdpi.com/2073-4441/15/23/4106 |
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| author | Simone Becarelli Giacomo Bernabei Giovanna Siracusa Diego Baderna Monica Ruffini Castiglione Giampiero De Simone Simona Di Gregorio |
| author_facet | Simone Becarelli Giacomo Bernabei Giovanna Siracusa Diego Baderna Monica Ruffini Castiglione Giampiero De Simone Simona Di Gregorio |
| author_sort | Simone Becarelli |
| collection | DOAJ |
| description | To accelerate the depletion of total petroleum hydrocarbons, a hydrocarburoclastic ascomycetes, <i>Lambertella</i> sp. MUT 5852, was bioaugmented to dredged sediments co-composting with a lignocellulosic matrix. After only 28 days of incubation, a complete depletion of the contamination was observed. The 16S rDNA metabarcoding of the bacterial community and a predictive functional metagenomic analysis were adopted to evaluate potential bacterial degrading and detoxifying functions. A combination of toxicological assays on two eukaryotic models, the root tips of <i>Vicia faba</i> and the human intestinal epithelial Caco-2 cells, was adopted to assess the robustness of the process not only for the decontamination but also for the detoxification of the dredged sediments. Bacterial taxa, such as <i>Kocuria</i> and <i>Sphingobacterium</i> sps., resulted to be involved in both the decontamination and detoxification of the co-composting dredged sediments by potential activation of diverse oxidative processes. At the same time, the <i>Kocuria</i> sp. showed plant growth-promoting activity by the potential expression of the 1-aminocyclopropane-1-carboxylate deaminase activity, providing functional traits of interest for a technosol in terms of sustaining primary producer growth and development. |
| first_indexed | 2024-03-09T01:40:44Z |
| format | Article |
| id | doaj.art-1aa8768969a74429b73281881c42d050 |
| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-09T01:40:44Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-1aa8768969a74429b73281881c42d0502023-12-08T15:28:29ZengMDPI AGWater2073-44412023-11-011523410610.3390/w15234106Bio-Based Decontamination and Detoxification of Total Petroleum Hydrocarbon-Contaminated Dredged Sediments: Perspectives to Produce Constructed Technosols in the Frame of the Circular EconomySimone Becarelli0Giacomo Bernabei1Giovanna Siracusa2Diego Baderna3Monica Ruffini Castiglione4Giampiero De Simone5Simona Di Gregorio6Department of Biology, University of Pisa, 56126 Pisa, ItalyDepartment of Biology, University of Pisa, 56126 Pisa, ItalyDepartment of Biology, University of Pisa, 56126 Pisa, ItalyChemservice s.r.l.—Lab Analysis Group, Via F.lli Beltrami, 15, Novate Milanese, 20026 Milan, ItalyDepartment of Biology, University of Pisa, 56126 Pisa, ItalyDepartment of Biology, University of Pisa, 56126 Pisa, ItalyDepartment of Biology, University of Pisa, 56126 Pisa, ItalyTo accelerate the depletion of total petroleum hydrocarbons, a hydrocarburoclastic ascomycetes, <i>Lambertella</i> sp. MUT 5852, was bioaugmented to dredged sediments co-composting with a lignocellulosic matrix. After only 28 days of incubation, a complete depletion of the contamination was observed. The 16S rDNA metabarcoding of the bacterial community and a predictive functional metagenomic analysis were adopted to evaluate potential bacterial degrading and detoxifying functions. A combination of toxicological assays on two eukaryotic models, the root tips of <i>Vicia faba</i> and the human intestinal epithelial Caco-2 cells, was adopted to assess the robustness of the process not only for the decontamination but also for the detoxification of the dredged sediments. Bacterial taxa, such as <i>Kocuria</i> and <i>Sphingobacterium</i> sps., resulted to be involved in both the decontamination and detoxification of the co-composting dredged sediments by potential activation of diverse oxidative processes. At the same time, the <i>Kocuria</i> sp. showed plant growth-promoting activity by the potential expression of the 1-aminocyclopropane-1-carboxylate deaminase activity, providing functional traits of interest for a technosol in terms of sustaining primary producer growth and development.https://www.mdpi.com/2073-4441/15/23/4106mycoremediation<i>Lambertella</i> sp. MUT 5852predictive functional metagenomic analysis<i>Kocuria</i> sp.<i>Sphingobacterium</i> sp. |
| spellingShingle | Simone Becarelli Giacomo Bernabei Giovanna Siracusa Diego Baderna Monica Ruffini Castiglione Giampiero De Simone Simona Di Gregorio Bio-Based Decontamination and Detoxification of Total Petroleum Hydrocarbon-Contaminated Dredged Sediments: Perspectives to Produce Constructed Technosols in the Frame of the Circular Economy Water mycoremediation <i>Lambertella</i> sp. MUT 5852 predictive functional metagenomic analysis <i>Kocuria</i> sp. <i>Sphingobacterium</i> sp. |
| title | Bio-Based Decontamination and Detoxification of Total Petroleum Hydrocarbon-Contaminated Dredged Sediments: Perspectives to Produce Constructed Technosols in the Frame of the Circular Economy |
| title_full | Bio-Based Decontamination and Detoxification of Total Petroleum Hydrocarbon-Contaminated Dredged Sediments: Perspectives to Produce Constructed Technosols in the Frame of the Circular Economy |
| title_fullStr | Bio-Based Decontamination and Detoxification of Total Petroleum Hydrocarbon-Contaminated Dredged Sediments: Perspectives to Produce Constructed Technosols in the Frame of the Circular Economy |
| title_full_unstemmed | Bio-Based Decontamination and Detoxification of Total Petroleum Hydrocarbon-Contaminated Dredged Sediments: Perspectives to Produce Constructed Technosols in the Frame of the Circular Economy |
| title_short | Bio-Based Decontamination and Detoxification of Total Petroleum Hydrocarbon-Contaminated Dredged Sediments: Perspectives to Produce Constructed Technosols in the Frame of the Circular Economy |
| title_sort | bio based decontamination and detoxification of total petroleum hydrocarbon contaminated dredged sediments perspectives to produce constructed technosols in the frame of the circular economy |
| topic | mycoremediation <i>Lambertella</i> sp. MUT 5852 predictive functional metagenomic analysis <i>Kocuria</i> sp. <i>Sphingobacterium</i> sp. |
| url | https://www.mdpi.com/2073-4441/15/23/4106 |
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