Transition of microbiota in chicken cecal droppings from commercial broiler farms
Abstract Background Chickens are major sources of human nutrition worldwide, but the chicken intestinal microbiota can be a source of bacterial infection. The microbiota has potential to regulate the colonization of pathogens by competitive exclusion, production of antimicrobial compounds, and stimu...
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| Format: | Article |
| Language: | English |
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BMC
2021-01-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-020-02688-7 |
| _version_ | 1818580156100378624 |
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| author | Nachiko Takeshita Takayasu Watanabe Kasumi Ishida-Kuroki Tsutomu Sekizaki |
| author_facet | Nachiko Takeshita Takayasu Watanabe Kasumi Ishida-Kuroki Tsutomu Sekizaki |
| author_sort | Nachiko Takeshita |
| collection | DOAJ |
| description | Abstract Background Chickens are major sources of human nutrition worldwide, but the chicken intestinal microbiota can be a source of bacterial infection. The microbiota has potential to regulate the colonization of pathogens by competitive exclusion, production of antimicrobial compounds, and stimulation of the mucosal immune system. But information on the microbiota in commercial broiler chickens is limited because of the difficulty of conducting studies at commercial farms. To obtain fundamental information that can be used to control pathogens in chickens, we determined the 6-week dynamics of microbiota in chicken cecal droppings from commercial broiler farms. Results Cecal droppings from four chickens were collected once a week from 1 to 6 weeks of age at three commercial broiler farms. A total of 168 samples were collected from 7 flocks and subjected to 16S rRNA amplicon sequencing. Despite the farms have distinctly different climate conditions, the microbiota in the same growth stages were similar among farms. Moreover, as the chickens grew and the feed types were switched, the richness and diversity of the microbiota gradually increased and convergence of the composition of the microbiota was apparent. Notably, minor bacterial taxa (i.e. OTUs with relative abundance < 0.05%) within the microbiota were changed by the chicken age, switching of feed types, and presence of Campylobacter. In particular, the effects of switching of feed types on the microbiota were larger than the effects of age and Campylobacter. Conclusions Irrespective of the locations of the farms, the microbiota of chicken cecum, especially minor bacteria, was successively changed more affected by feed types than by ages. Switching of feed types inducing the alteration of the microbiota may be associated with the colonization of pathogens in the chicken gut. These results will also help with extrapolation of studies in experimental animals to those in the commercial farms. |
| first_indexed | 2024-12-16T07:13:07Z |
| format | Article |
| id | doaj.art-1aa8d2a0c48f496eb61cbe250423aa75 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-16T07:13:07Z |
| publishDate | 2021-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-1aa8d2a0c48f496eb61cbe250423aa752022-12-21T22:39:51ZengBMCBMC Veterinary Research1746-61482021-01-0117111110.1186/s12917-020-02688-7Transition of microbiota in chicken cecal droppings from commercial broiler farmsNachiko Takeshita0Takayasu Watanabe1Kasumi Ishida-Kuroki2Tsutomu Sekizaki3Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of TokyoResearch Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of TokyoResearch Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of TokyoResearch Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of TokyoAbstract Background Chickens are major sources of human nutrition worldwide, but the chicken intestinal microbiota can be a source of bacterial infection. The microbiota has potential to regulate the colonization of pathogens by competitive exclusion, production of antimicrobial compounds, and stimulation of the mucosal immune system. But information on the microbiota in commercial broiler chickens is limited because of the difficulty of conducting studies at commercial farms. To obtain fundamental information that can be used to control pathogens in chickens, we determined the 6-week dynamics of microbiota in chicken cecal droppings from commercial broiler farms. Results Cecal droppings from four chickens were collected once a week from 1 to 6 weeks of age at three commercial broiler farms. A total of 168 samples were collected from 7 flocks and subjected to 16S rRNA amplicon sequencing. Despite the farms have distinctly different climate conditions, the microbiota in the same growth stages were similar among farms. Moreover, as the chickens grew and the feed types were switched, the richness and diversity of the microbiota gradually increased and convergence of the composition of the microbiota was apparent. Notably, minor bacterial taxa (i.e. OTUs with relative abundance < 0.05%) within the microbiota were changed by the chicken age, switching of feed types, and presence of Campylobacter. In particular, the effects of switching of feed types on the microbiota were larger than the effects of age and Campylobacter. Conclusions Irrespective of the locations of the farms, the microbiota of chicken cecum, especially minor bacteria, was successively changed more affected by feed types than by ages. Switching of feed types inducing the alteration of the microbiota may be associated with the colonization of pathogens in the chicken gut. These results will also help with extrapolation of studies in experimental animals to those in the commercial farms.https://doi.org/10.1186/s12917-020-02688-716S rRNA amplicon sequenceBroiler chickensCecal droppingCampylobacterSalmonella |
| spellingShingle | Nachiko Takeshita Takayasu Watanabe Kasumi Ishida-Kuroki Tsutomu Sekizaki Transition of microbiota in chicken cecal droppings from commercial broiler farms BMC Veterinary Research 16S rRNA amplicon sequence Broiler chickens Cecal dropping Campylobacter Salmonella |
| title | Transition of microbiota in chicken cecal droppings from commercial broiler farms |
| title_full | Transition of microbiota in chicken cecal droppings from commercial broiler farms |
| title_fullStr | Transition of microbiota in chicken cecal droppings from commercial broiler farms |
| title_full_unstemmed | Transition of microbiota in chicken cecal droppings from commercial broiler farms |
| title_short | Transition of microbiota in chicken cecal droppings from commercial broiler farms |
| title_sort | transition of microbiota in chicken cecal droppings from commercial broiler farms |
| topic | 16S rRNA amplicon sequence Broiler chickens Cecal dropping Campylobacter Salmonella |
| url | https://doi.org/10.1186/s12917-020-02688-7 |
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