Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology
Abstract Cellular organization and functions encompass multiple scales in vivo. Emerging high-plex imaging technologies are limited in resolving subcellular biomolecular features. Expansion Microscopy (ExM) and related techniques physically expand samples for enhanced spatial resolution, but are cha...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-07-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-39616-w |
| _version_ | 1827905345936162816 |
|---|---|
| author | Yunhao Bai Bokai Zhu John-Paul Oliveria Bryan J. Cannon Dorien Feyaerts Marc Bosse Kausalia Vijayaragavan Noah F. Greenwald Darci Phillips Christian M. Schürch Samuel M. Naik Edward A. Ganio Brice Gaudilliere Scott J. Rodig Michael B. Miller Michael Angelo Sean C. Bendall Xavier Rovira-Clavé Garry P. Nolan Sizun Jiang |
| author_facet | Yunhao Bai Bokai Zhu John-Paul Oliveria Bryan J. Cannon Dorien Feyaerts Marc Bosse Kausalia Vijayaragavan Noah F. Greenwald Darci Phillips Christian M. Schürch Samuel M. Naik Edward A. Ganio Brice Gaudilliere Scott J. Rodig Michael B. Miller Michael Angelo Sean C. Bendall Xavier Rovira-Clavé Garry P. Nolan Sizun Jiang |
| author_sort | Yunhao Bai |
| collection | DOAJ |
| description | Abstract Cellular organization and functions encompass multiple scales in vivo. Emerging high-plex imaging technologies are limited in resolving subcellular biomolecular features. Expansion Microscopy (ExM) and related techniques physically expand samples for enhanced spatial resolution, but are challenging to be combined with high-plex imaging technologies to enable integrative multiscaled tissue biology insights. Here, we introduce Expand and comPRESS hydrOgels (ExPRESSO), an ExM framework that allows high-plex protein staining, physical expansion, and removal of water, while retaining the lateral tissue expansion. We demonstrate ExPRESSO imaging of archival clinical tissue samples on Multiplexed Ion Beam Imaging and Imaging Mass Cytometry platforms, with detection capabilities of > 40 markers. Application of ExPRESSO on archival human lymphoid and brain tissues resolved tissue architecture at the subcellular level, particularly that of the blood-brain barrier. ExPRESSO hence provides a platform for extending the analysis compatibility of hydrogel-expanded biospecimens to mass spectrometry, with minimal modifications to protocols and instrumentation. |
| first_indexed | 2024-03-13T00:41:20Z |
| format | Article |
| id | doaj.art-1aaa853ff55a4176b7b32d00970f18f6 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-13T00:41:20Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-1aaa853ff55a4176b7b32d00970f18f62023-07-09T11:17:40ZengNature PortfolioNature Communications2041-17232023-07-0114111810.1038/s41467-023-39616-wExpanded vacuum-stable gels for multiplexed high-resolution spatial histopathologyYunhao Bai0Bokai Zhu1John-Paul Oliveria2Bryan J. Cannon3Dorien Feyaerts4Marc Bosse5Kausalia Vijayaragavan6Noah F. Greenwald7Darci Phillips8Christian M. Schürch9Samuel M. Naik10Edward A. Ganio11Brice Gaudilliere12Scott J. Rodig13Michael B. Miller14Michael Angelo15Sean C. Bendall16Xavier Rovira-Clavé17Garry P. Nolan18Sizun Jiang19Department of Pathology, Stanford UniversityDepartment of Pathology, Stanford UniversityDepartment of Translational Medicine, Genentech, Inc.Department of Pathology, Stanford UniversityDepartment of Anesthesiology, Perioperative and Pain Medicine, Stanford UniversityDepartment of Pathology, Stanford UniversityDepartment of Pathology, Stanford UniversityDepartment of Pathology, Stanford UniversityDepartment of Pathology, Stanford UniversityDepartment of Pathology, Stanford UniversityDepartment of Pathology, Brigham and Women’s Hospital, Harvard Medical SchoolDepartment of Anesthesiology, Perioperative and Pain Medicine, Stanford UniversityDepartment of Anesthesiology, Perioperative and Pain Medicine, Stanford UniversityDepartment of Pathology, Brigham and Women’s Hospital, Harvard Medical SchoolDepartment of Pathology, Brigham and Women’s Hospital, Harvard Medical SchoolDepartment of Pathology, Stanford UniversityDepartment of Pathology, Stanford UniversityDepartment of Pathology, Stanford UniversityDepartment of Pathology, Stanford UniversityDepartment of Pathology, Stanford UniversityAbstract Cellular organization and functions encompass multiple scales in vivo. Emerging high-plex imaging technologies are limited in resolving subcellular biomolecular features. Expansion Microscopy (ExM) and related techniques physically expand samples for enhanced spatial resolution, but are challenging to be combined with high-plex imaging technologies to enable integrative multiscaled tissue biology insights. Here, we introduce Expand and comPRESS hydrOgels (ExPRESSO), an ExM framework that allows high-plex protein staining, physical expansion, and removal of water, while retaining the lateral tissue expansion. We demonstrate ExPRESSO imaging of archival clinical tissue samples on Multiplexed Ion Beam Imaging and Imaging Mass Cytometry platforms, with detection capabilities of > 40 markers. Application of ExPRESSO on archival human lymphoid and brain tissues resolved tissue architecture at the subcellular level, particularly that of the blood-brain barrier. ExPRESSO hence provides a platform for extending the analysis compatibility of hydrogel-expanded biospecimens to mass spectrometry, with minimal modifications to protocols and instrumentation.https://doi.org/10.1038/s41467-023-39616-w |
| spellingShingle | Yunhao Bai Bokai Zhu John-Paul Oliveria Bryan J. Cannon Dorien Feyaerts Marc Bosse Kausalia Vijayaragavan Noah F. Greenwald Darci Phillips Christian M. Schürch Samuel M. Naik Edward A. Ganio Brice Gaudilliere Scott J. Rodig Michael B. Miller Michael Angelo Sean C. Bendall Xavier Rovira-Clavé Garry P. Nolan Sizun Jiang Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology Nature Communications |
| title | Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology |
| title_full | Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology |
| title_fullStr | Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology |
| title_full_unstemmed | Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology |
| title_short | Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology |
| title_sort | expanded vacuum stable gels for multiplexed high resolution spatial histopathology |
| url | https://doi.org/10.1038/s41467-023-39616-w |
| work_keys_str_mv | AT yunhaobai expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT bokaizhu expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT johnpauloliveria expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT bryanjcannon expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT dorienfeyaerts expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT marcbosse expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT kausaliavijayaragavan expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT noahfgreenwald expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT darciphillips expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT christianmschurch expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT samuelmnaik expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT edwardaganio expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT bricegaudilliere expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT scottjrodig expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT michaelbmiller expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT michaelangelo expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT seancbendall expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT xavierroviraclave expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT garrypnolan expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology AT sizunjiang expandedvacuumstablegelsformultiplexedhighresolutionspatialhistopathology |