The transcription factor GhWRKY70 from gossypium hirsutum enhances resistance to verticillium wilt via the jasmonic acid pathway
Abstract Background The WRKY transcription factors play significant roles in plant growth, development, and defense responses. However, in cotton, the molecular mechanism of most WRKY proteins and their involvement in Verticillium wilt tolerance are not well understood. Results GhWRKY70 is greatly u...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04141-x |
| _version_ | 1827984701961273344 |
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| author | Shuling Zhang Lijun Dong Xue Zhang Xiaohong Fu Lin Zhao Lizhu Wu Xingfen Wang Jianfeng Liu |
| author_facet | Shuling Zhang Lijun Dong Xue Zhang Xiaohong Fu Lin Zhao Lizhu Wu Xingfen Wang Jianfeng Liu |
| author_sort | Shuling Zhang |
| collection | DOAJ |
| description | Abstract Background The WRKY transcription factors play significant roles in plant growth, development, and defense responses. However, in cotton, the molecular mechanism of most WRKY proteins and their involvement in Verticillium wilt tolerance are not well understood. Results GhWRKY70 is greatly up-regulated in cotton by Verticillium dahliae. Subcellular localization suggests that GhWRKY70 is only located in the nucleus. Transcriptional activation of GhWRKY70 further demonstrates that GhWRKY70 function as a transcriptional activator. Transgenic Arabidopsis plants overexpressing GhWRKY70 exhibited better growth performance and higher lignin content, antioxidant enzyme activities and jasmonic acid (JA) levels than wild-type plants after infection with V. dahliae. In addition, the transgenic Arabidopsis resulted in an enhanced expression level of AtAOS1, a gene related to JA synthesis, further leading to a higher JA accumulation compared to the wild type. However, the disease index (DI) values of the VIGS-treated cotton plants with TRV:WRKY70 were also significantly higher than those of the VIGS-treated cotton plants with TRV:00. The chlorophyll and lignin contents of TRV:WRKY70 plants were significantly lower than those of TRV:00 plants. GhAOS1 expression and JA abundance in TRV:WRKY70 plants were decreased. The GhWRKY70 protein was confirmed to bind to the W-box element in the promoter region of GhAOS by yeast one-hybrid assay and transient expression. Conclusion These results indicate that the GhWRKY70 transcription factor is a positive regulator in Verticillium wilt tolerance of cotton, and may promote the production of JA via regulation of GhAOS1 expression. |
| first_indexed | 2024-04-09T23:03:45Z |
| format | Article |
| id | doaj.art-1aaa89e99829433a8136f29a460b566d |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-09T23:03:45Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-1aaa89e99829433a8136f29a460b566d2023-03-22T10:49:54ZengBMCBMC Plant Biology1471-22292023-03-0123111510.1186/s12870-023-04141-xThe transcription factor GhWRKY70 from gossypium hirsutum enhances resistance to verticillium wilt via the jasmonic acid pathwayShuling Zhang0Lijun Dong1Xue Zhang2Xiaohong Fu3Lin Zhao4Lizhu Wu5Xingfen Wang6Jianfeng Liu7School of Life Sciences, Institute of Life Science and Green Development, Hebei UniversitySchool of Life Sciences, Institute of Life Science and Green Development, Hebei UniversitySchool of Life Sciences, Institute of Life Science and Green Development, Hebei UniversitySchool of Life Sciences, Institute of Life Science and Green Development, Hebei UniversitySchool of Life Sciences, Institute of Life Science and Green Development, Hebei UniversityState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural UniversityState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural UniversitySchool of Life Sciences, Institute of Life Science and Green Development, Hebei UniversityAbstract Background The WRKY transcription factors play significant roles in plant growth, development, and defense responses. However, in cotton, the molecular mechanism of most WRKY proteins and their involvement in Verticillium wilt tolerance are not well understood. Results GhWRKY70 is greatly up-regulated in cotton by Verticillium dahliae. Subcellular localization suggests that GhWRKY70 is only located in the nucleus. Transcriptional activation of GhWRKY70 further demonstrates that GhWRKY70 function as a transcriptional activator. Transgenic Arabidopsis plants overexpressing GhWRKY70 exhibited better growth performance and higher lignin content, antioxidant enzyme activities and jasmonic acid (JA) levels than wild-type plants after infection with V. dahliae. In addition, the transgenic Arabidopsis resulted in an enhanced expression level of AtAOS1, a gene related to JA synthesis, further leading to a higher JA accumulation compared to the wild type. However, the disease index (DI) values of the VIGS-treated cotton plants with TRV:WRKY70 were also significantly higher than those of the VIGS-treated cotton plants with TRV:00. The chlorophyll and lignin contents of TRV:WRKY70 plants were significantly lower than those of TRV:00 plants. GhAOS1 expression and JA abundance in TRV:WRKY70 plants were decreased. The GhWRKY70 protein was confirmed to bind to the W-box element in the promoter region of GhAOS by yeast one-hybrid assay and transient expression. Conclusion These results indicate that the GhWRKY70 transcription factor is a positive regulator in Verticillium wilt tolerance of cotton, and may promote the production of JA via regulation of GhAOS1 expression.https://doi.org/10.1186/s12870-023-04141-xCottonTranscription factorGhWRKY70Verticillium wiltJasmonic acid pathway |
| spellingShingle | Shuling Zhang Lijun Dong Xue Zhang Xiaohong Fu Lin Zhao Lizhu Wu Xingfen Wang Jianfeng Liu The transcription factor GhWRKY70 from gossypium hirsutum enhances resistance to verticillium wilt via the jasmonic acid pathway BMC Plant Biology Cotton Transcription factor GhWRKY70 Verticillium wilt Jasmonic acid pathway |
| title | The transcription factor GhWRKY70 from gossypium hirsutum enhances resistance to verticillium wilt via the jasmonic acid pathway |
| title_full | The transcription factor GhWRKY70 from gossypium hirsutum enhances resistance to verticillium wilt via the jasmonic acid pathway |
| title_fullStr | The transcription factor GhWRKY70 from gossypium hirsutum enhances resistance to verticillium wilt via the jasmonic acid pathway |
| title_full_unstemmed | The transcription factor GhWRKY70 from gossypium hirsutum enhances resistance to verticillium wilt via the jasmonic acid pathway |
| title_short | The transcription factor GhWRKY70 from gossypium hirsutum enhances resistance to verticillium wilt via the jasmonic acid pathway |
| title_sort | transcription factor ghwrky70 from gossypium hirsutum enhances resistance to verticillium wilt via the jasmonic acid pathway |
| topic | Cotton Transcription factor GhWRKY70 Verticillium wilt Jasmonic acid pathway |
| url | https://doi.org/10.1186/s12870-023-04141-x |
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