High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised <i>Trametes hirsuta</i> PW17-41 and FTIR Analysis
Coloured wastewater from the textile industry is a very serious global problem. Among 16 different white-rot fungal isolates, <i>Trametes hirsuta</i> PW17-41 revealed high potential for decolourisation of mixed textile dyes (Navy EC-R, Ruby S3B and Super Black G) from real industrial was...
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MDPI AG
2022-05-01
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| Series: | Microorganisms |
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| author | Bancha Thampraphaphon Cherdchai Phosri Nipon Pisutpaisal Pisit Thamvithayakorn Kruawan Chotelersak Sarper Sarp Nuttika Suwannasai |
| author_facet | Bancha Thampraphaphon Cherdchai Phosri Nipon Pisutpaisal Pisit Thamvithayakorn Kruawan Chotelersak Sarper Sarp Nuttika Suwannasai |
| author_sort | Bancha Thampraphaphon |
| collection | DOAJ |
| description | Coloured wastewater from the textile industry is a very serious global problem. Among 16 different white-rot fungal isolates, <i>Trametes hirsuta</i> PW17-41 revealed high potential for decolourisation of mixed textile dyes (Navy EC-R, Ruby S3B and Super Black G) from real industrial wastewater samples. The efficiency of dye decolourisation was evaluated using the American Dye Manufacturers’ Institute (ADMI) standard methodology. The suitable support for fungal mycelium immobilisation was nylon sponges. The optimal dye decolourisation (95.39%) was achieved by using palm sugar and ammonium nitrate as carbon and nitrogen sources, respectively. The initial pH was 5 and the agitation speed was 100 rpm at 30 °C. The ADMI values of textile dyes decreased from 2475 to 114 within two days, reducing the treatment time from seven days before optimisation. The major mechanism of dye decolourisation was biodegradation, which was confirmed by UV–visible and FTIR spectra. Manganese peroxidase (MnP) (4942 U L<sup>−1</sup>) was found to be the main enzyme during the decolourisation process at an initial dye concentration of 21,200 ADMI. The results indicated the strong potential of immobilised fungal cells to remove high concentrations of textile dyes from industrial wastewater and their potential ability to produce high MnP and laccase activities that can be used in further application. |
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| last_indexed | 2024-03-10T03:21:47Z |
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| series | Microorganisms |
| spelling | doaj.art-1aac703a4d1846849710ec44c9ff6f392023-11-23T12:16:10ZengMDPI AGMicroorganisms2076-26072022-05-0110599210.3390/microorganisms10050992High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised <i>Trametes hirsuta</i> PW17-41 and FTIR AnalysisBancha Thampraphaphon0Cherdchai Phosri1Nipon Pisutpaisal2Pisit Thamvithayakorn3Kruawan Chotelersak4Sarper Sarp5Nuttika Suwannasai6Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, ThailandDepartment of Biology, Faculty of Science, Nakhon Phanom University, Nakhon Phanom 48000, ThailandDepartment of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangkok 10800, ThailandDepartment of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, ThailandDepartment of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, ThailandCentre for Water Advanced Technologies and Environmental Research (CWATER), College of Engineering, Swansea University, Fabian Way, Swansea SA1 8EN, UKDepartment of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, ThailandColoured wastewater from the textile industry is a very serious global problem. Among 16 different white-rot fungal isolates, <i>Trametes hirsuta</i> PW17-41 revealed high potential for decolourisation of mixed textile dyes (Navy EC-R, Ruby S3B and Super Black G) from real industrial wastewater samples. The efficiency of dye decolourisation was evaluated using the American Dye Manufacturers’ Institute (ADMI) standard methodology. The suitable support for fungal mycelium immobilisation was nylon sponges. The optimal dye decolourisation (95.39%) was achieved by using palm sugar and ammonium nitrate as carbon and nitrogen sources, respectively. The initial pH was 5 and the agitation speed was 100 rpm at 30 °C. The ADMI values of textile dyes decreased from 2475 to 114 within two days, reducing the treatment time from seven days before optimisation. The major mechanism of dye decolourisation was biodegradation, which was confirmed by UV–visible and FTIR spectra. Manganese peroxidase (MnP) (4942 U L<sup>−1</sup>) was found to be the main enzyme during the decolourisation process at an initial dye concentration of 21,200 ADMI. The results indicated the strong potential of immobilised fungal cells to remove high concentrations of textile dyes from industrial wastewater and their potential ability to produce high MnP and laccase activities that can be used in further application.https://www.mdpi.com/2076-2607/10/5/992textile dyesdecolourisationwhite-rot fungimanganese peroxidaseimmobilisationADMI |
| spellingShingle | Bancha Thampraphaphon Cherdchai Phosri Nipon Pisutpaisal Pisit Thamvithayakorn Kruawan Chotelersak Sarper Sarp Nuttika Suwannasai High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised <i>Trametes hirsuta</i> PW17-41 and FTIR Analysis Microorganisms textile dyes decolourisation white-rot fungi manganese peroxidase immobilisation ADMI |
| title | High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised <i>Trametes hirsuta</i> PW17-41 and FTIR Analysis |
| title_full | High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised <i>Trametes hirsuta</i> PW17-41 and FTIR Analysis |
| title_fullStr | High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised <i>Trametes hirsuta</i> PW17-41 and FTIR Analysis |
| title_full_unstemmed | High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised <i>Trametes hirsuta</i> PW17-41 and FTIR Analysis |
| title_short | High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised <i>Trametes hirsuta</i> PW17-41 and FTIR Analysis |
| title_sort | high potential decolourisation of textile dyes from wastewater by manganese peroxidase production of newly immobilised i trametes hirsuta i pw17 41 and ftir analysis |
| topic | textile dyes decolourisation white-rot fungi manganese peroxidase immobilisation ADMI |
| url | https://www.mdpi.com/2076-2607/10/5/992 |
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