Wavelength dependent excited state dynamics observed in canonical pyrimidine nucleosides
Epidemiological evidence indicates that damage to DNA/RNA initialized by ultraviolet (UV) radiation is associated with skin cancer. Wavelength dependence of DNA photodamage was proposed as early as 1990s and demonstrated later on. Unraveling the photo-activated dynamics involved in related reactions...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Journal of Photochemistry and Photobiology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666469023000520 |
| _version_ | 1827596305565745152 |
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| author | Peicong Wu Xueli Wang Haifeng Pan Jinquan Chen |
| author_facet | Peicong Wu Xueli Wang Haifeng Pan Jinquan Chen |
| author_sort | Peicong Wu |
| collection | DOAJ |
| description | Epidemiological evidence indicates that damage to DNA/RNA initialized by ultraviolet (UV) radiation is associated with skin cancer. Wavelength dependence of DNA photodamage was proposed as early as 1990s and demonstrated later on. Unraveling the photo-activated dynamics involved in related reactions is essential. However, studies aimed at uncovering the wavelength dependent excited state dynamics in canonical pyrimidine nucleosides have not received enough attention. In this work, excitation wavelength dependent excited state dynamics of 2′-deoxy-thymidine (dThd) and oxy-uridine (Urd) are investigated in acetonitrile solutions by femtosecond broadband transient absorption spectroscopy. Varying the excitation wavelength leads to a significant difference in the branching of the excited state population at the Franck-Condon (FC) region, resulting higher fluorescence quantum yield with 285 nm pump but higher triplet state quantum yield under 267 nm excitation. Based on our results, a vibronic coupling regulated excited state relaxation mechanism is proposed. This mechanism information is important for understanding the formation of harmful photoproducts for DNA/RNA with different wavelength UV excitations. |
| first_indexed | 2024-03-09T03:08:43Z |
| format | Article |
| id | doaj.art-1ab0b808d4b8475e9813095dfce5c789 |
| institution | Directory Open Access Journal |
| issn | 2666-4690 |
| language | English |
| last_indexed | 2024-03-09T03:08:43Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Photochemistry and Photobiology |
| spelling | doaj.art-1ab0b808d4b8475e9813095dfce5c7892023-12-04T05:24:14ZengElsevierJournal of Photochemistry and Photobiology2666-46902023-12-0118100211Wavelength dependent excited state dynamics observed in canonical pyrimidine nucleosidesPeicong Wu0Xueli Wang1Haifeng Pan2Jinquan Chen3State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, ChinaState Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, China; Corresponding authors.State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, ChinaState Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, China; Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan, Shanxi 030006, China; Corresponding authors.Epidemiological evidence indicates that damage to DNA/RNA initialized by ultraviolet (UV) radiation is associated with skin cancer. Wavelength dependence of DNA photodamage was proposed as early as 1990s and demonstrated later on. Unraveling the photo-activated dynamics involved in related reactions is essential. However, studies aimed at uncovering the wavelength dependent excited state dynamics in canonical pyrimidine nucleosides have not received enough attention. In this work, excitation wavelength dependent excited state dynamics of 2′-deoxy-thymidine (dThd) and oxy-uridine (Urd) are investigated in acetonitrile solutions by femtosecond broadband transient absorption spectroscopy. Varying the excitation wavelength leads to a significant difference in the branching of the excited state population at the Franck-Condon (FC) region, resulting higher fluorescence quantum yield with 285 nm pump but higher triplet state quantum yield under 267 nm excitation. Based on our results, a vibronic coupling regulated excited state relaxation mechanism is proposed. This mechanism information is important for understanding the formation of harmful photoproducts for DNA/RNA with different wavelength UV excitations.http://www.sciencedirect.com/science/article/pii/S2666469023000520DNA photodynamicsExcited state dynamicsPyrimidineWavelength dependent |
| spellingShingle | Peicong Wu Xueli Wang Haifeng Pan Jinquan Chen Wavelength dependent excited state dynamics observed in canonical pyrimidine nucleosides Journal of Photochemistry and Photobiology DNA photodynamics Excited state dynamics Pyrimidine Wavelength dependent |
| title | Wavelength dependent excited state dynamics observed in canonical pyrimidine nucleosides |
| title_full | Wavelength dependent excited state dynamics observed in canonical pyrimidine nucleosides |
| title_fullStr | Wavelength dependent excited state dynamics observed in canonical pyrimidine nucleosides |
| title_full_unstemmed | Wavelength dependent excited state dynamics observed in canonical pyrimidine nucleosides |
| title_short | Wavelength dependent excited state dynamics observed in canonical pyrimidine nucleosides |
| title_sort | wavelength dependent excited state dynamics observed in canonical pyrimidine nucleosides |
| topic | DNA photodynamics Excited state dynamics Pyrimidine Wavelength dependent |
| url | http://www.sciencedirect.com/science/article/pii/S2666469023000520 |
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