Identification of potential bioactive compounds of Passiflora edulis leaf extract against colon adenocarcinoma cells
Plant bioactive compounds such as flavonoids and triterpenes can affect lipid metabolism. Here, we report the cytotoxic and lipid-lowering activities of the ethanolic extract of P. edulis leaves on human colon adenocarcinoma SW480 cells, also the molecular interactions of bioactive compounds present...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580823000341 |
| _version_ | 1797813710929002496 |
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| author | Johan Alexander Villada Ramos Johanny Aguillón Osma Beatriz Restrepo Cortes Nelsy Loango Chamarro María Elena Maldonado Celis |
| author_facet | Johan Alexander Villada Ramos Johanny Aguillón Osma Beatriz Restrepo Cortes Nelsy Loango Chamarro María Elena Maldonado Celis |
| author_sort | Johan Alexander Villada Ramos |
| collection | DOAJ |
| description | Plant bioactive compounds such as flavonoids and triterpenes can affect lipid metabolism. Here, we report the cytotoxic and lipid-lowering activities of the ethanolic extract of P. edulis leaves on human colon adenocarcinoma SW480 cells, also the molecular interactions of bioactive compounds present in P. edulis extract on ACC and HMGCR enzymes. The extract reduced cell viability and decreased intracellular triglyceride content by up to 35% and 28% at 24 and 48 h, respectively; whereas the effect was evident on cholesterol only at 24 h. In-silico analysis revealed that luteolin, chlorogenic acid, moupinamide, isoorientin, glucosyl passionflower, cyclopasifloic acid E and saponarin had optimal molecular coupling on Acetyl-CoA Carboxylase 1 and 2 as well as 3-hydroxy-3-methyl-glutaryl-CoA reductase, with possible inhibitory effects. These results show the ability of ethanolic extract to reduce intracellular levels of cholesterol and triglycerides in SW480 cells, which attracts attention for the treatment of colorectal cancer. |
| first_indexed | 2024-03-13T07:56:47Z |
| format | Article |
| id | doaj.art-1ab445f7f64946f9b3bf79de3437cded |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-03-13T07:56:47Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-1ab445f7f64946f9b3bf79de3437cded2023-06-02T04:23:23ZengElsevierBiochemistry and Biophysics Reports2405-58082023-07-0134101453Identification of potential bioactive compounds of Passiflora edulis leaf extract against colon adenocarcinoma cellsJohan Alexander Villada Ramos0Johanny Aguillón Osma1Beatriz Restrepo Cortes2Nelsy Loango Chamarro3María Elena Maldonado Celis4Facultad de Ciencias de la Salud, Universidad del Quindío, Armenia, Colombia; Facultad de Ciencias Básicas y Tecnologías, Universidad del Quindío, Armenia, ColombiaFacultad de Ciencias de la Salud, Universidad del Quindío, Armenia, Colombia; Facultad de Ciencias Básicas y Tecnologías, Universidad del Quindío, Armenia, ColombiaFacultad de Ciencias de la Salud, Universidad del Quindío, Armenia, ColombiaFacultad de Ciencias de la Salud, Universidad del Quindío, Armenia, Colombia; Facultad de Ciencias Básicas y Tecnologías, Universidad del Quindío, Armenia, ColombiaEscuela de Nutrición y Dietética, Universidad de Antioquia, Apartado, 1226, Medellín, Colombia; Corresponding author. Escuela de Nutrición y Dietética, Universidad de Antioquia, Carrera 75 #67-87, Medellín, Antioquia, Colombia.Plant bioactive compounds such as flavonoids and triterpenes can affect lipid metabolism. Here, we report the cytotoxic and lipid-lowering activities of the ethanolic extract of P. edulis leaves on human colon adenocarcinoma SW480 cells, also the molecular interactions of bioactive compounds present in P. edulis extract on ACC and HMGCR enzymes. The extract reduced cell viability and decreased intracellular triglyceride content by up to 35% and 28% at 24 and 48 h, respectively; whereas the effect was evident on cholesterol only at 24 h. In-silico analysis revealed that luteolin, chlorogenic acid, moupinamide, isoorientin, glucosyl passionflower, cyclopasifloic acid E and saponarin had optimal molecular coupling on Acetyl-CoA Carboxylase 1 and 2 as well as 3-hydroxy-3-methyl-glutaryl-CoA reductase, with possible inhibitory effects. These results show the ability of ethanolic extract to reduce intracellular levels of cholesterol and triglycerides in SW480 cells, which attracts attention for the treatment of colorectal cancer.http://www.sciencedirect.com/science/article/pii/S2405580823000341PassifloraLipid metabolismDocking molecular methodsColon cancerTriglyceridesCholesterol |
| spellingShingle | Johan Alexander Villada Ramos Johanny Aguillón Osma Beatriz Restrepo Cortes Nelsy Loango Chamarro María Elena Maldonado Celis Identification of potential bioactive compounds of Passiflora edulis leaf extract against colon adenocarcinoma cells Biochemistry and Biophysics Reports Passiflora Lipid metabolism Docking molecular methods Colon cancer Triglycerides Cholesterol |
| title | Identification of potential bioactive compounds of Passiflora edulis leaf extract against colon adenocarcinoma cells |
| title_full | Identification of potential bioactive compounds of Passiflora edulis leaf extract against colon adenocarcinoma cells |
| title_fullStr | Identification of potential bioactive compounds of Passiflora edulis leaf extract against colon adenocarcinoma cells |
| title_full_unstemmed | Identification of potential bioactive compounds of Passiflora edulis leaf extract against colon adenocarcinoma cells |
| title_short | Identification of potential bioactive compounds of Passiflora edulis leaf extract against colon adenocarcinoma cells |
| title_sort | identification of potential bioactive compounds of passiflora edulis leaf extract against colon adenocarcinoma cells |
| topic | Passiflora Lipid metabolism Docking molecular methods Colon cancer Triglycerides Cholesterol |
| url | http://www.sciencedirect.com/science/article/pii/S2405580823000341 |
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