Effects of Mild Hypothermia on Superoxide Anion Production, Superoxide Dismutase Expression, and Activity Following Transient Focal Cerebral Ischemia
Following a transient ischemic insult there is a marked increase in free radical (FR) production within the first 10–15 min of reperfusion and again at the peak of the inflammatory process. Hypothermia decreases lipid peroxidation following global ischemia, raising the possibility that it may act by...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2002-10-01
|
| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996102905139 |
| _version_ | 1829519130549551104 |
|---|---|
| author | Carolina M. Maier Guo Hua Sun Danye Cheng Midori A. Yenari Pak H. Chan Gary K. Steinberg |
| author_facet | Carolina M. Maier Guo Hua Sun Danye Cheng Midori A. Yenari Pak H. Chan Gary K. Steinberg |
| author_sort | Carolina M. Maier |
| collection | DOAJ |
| description | Following a transient ischemic insult there is a marked increase in free radical (FR) production within the first 10–15 min of reperfusion and again at the peak of the inflammatory process. Hypothermia decreases lipid peroxidation following global ischemia, raising the possibility that it may act by reducing FR production early on and by maintaining or increasing endogenous antioxidant systems. By means of FR fluorescence, Western blot, immunohistochemistry, and enzymatic assay, we studied the effects of mild hypothermia on superoxide (O−•2) anion production, superoxide dismutase SOD expression, and activity following focal cerebral ischemia in rats. Mild hypothermia significantly reduced O−•2 generation in the ischemic penumbra and corresponding contralateral region, but did not alter the bilateral SOD expression. SOD enzymatic activity in the ischemic core was slightly reduced in hypothermia-treated animals compared with normothermic controls. Our results suggest that the neuroprotective effect of mild hypothermia may be due, in part, to a reduction in neuronal and endothelial O−•2 production during early reperfusion. |
| first_indexed | 2024-12-16T14:33:57Z |
| format | Article |
| id | doaj.art-1ab8d3dca5364023a5b47087d9fd59d1 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-16T14:33:57Z |
| publishDate | 2002-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-1ab8d3dca5364023a5b47087d9fd59d12022-12-21T22:28:09ZengElsevierNeurobiology of Disease1095-953X2002-10-011112842Effects of Mild Hypothermia on Superoxide Anion Production, Superoxide Dismutase Expression, and Activity Following Transient Focal Cerebral IschemiaCarolina M. Maier0Guo Hua Sun1Danye Cheng2Midori A. Yenari3Pak H. Chan4Gary K. Steinberg5Department of Neurosurgery, Stanford University, Stanford, California, 94305-5487; Department of Neurology, Stanford University, Stanford, California, 94305-5487; Stanford Stroke Center, Stanford University, Stanford, California, 94305-5487Department of Neurosurgery, Stanford University, Stanford, California, 94305-5487; Department of Neurology, Stanford University, Stanford, California, 94305-5487; Stanford Stroke Center, Stanford University, Stanford, California, 94305-5487Department of Neurosurgery, Stanford University, Stanford, California, 94305-5487; Department of Neurology, Stanford University, Stanford, California, 94305-5487; Stanford Stroke Center, Stanford University, Stanford, California, 94305-5487Department of Neurosurgery, Stanford University, Stanford, California, 94305-5487; Department of Neurology, Stanford University, Stanford, California, 94305-5487; Stanford Stroke Center, Stanford University, Stanford, California, 94305-5487Department of Neurosurgery, Stanford University, Stanford, California, 94305-5487; Department of Neurology, Stanford University, Stanford, California, 94305-5487; Stanford Stroke Center, Stanford University, Stanford, California, 94305-5487Department of Neurosurgery, Stanford University, Stanford, California, 94305-5487; Department of Neurology, Stanford University, Stanford, California, 94305-5487; Stanford Stroke Center, Stanford University, Stanford, California, 94305-5487Following a transient ischemic insult there is a marked increase in free radical (FR) production within the first 10–15 min of reperfusion and again at the peak of the inflammatory process. Hypothermia decreases lipid peroxidation following global ischemia, raising the possibility that it may act by reducing FR production early on and by maintaining or increasing endogenous antioxidant systems. By means of FR fluorescence, Western blot, immunohistochemistry, and enzymatic assay, we studied the effects of mild hypothermia on superoxide (O−•2) anion production, superoxide dismutase SOD expression, and activity following focal cerebral ischemia in rats. Mild hypothermia significantly reduced O−•2 generation in the ischemic penumbra and corresponding contralateral region, but did not alter the bilateral SOD expression. SOD enzymatic activity in the ischemic core was slightly reduced in hypothermia-treated animals compared with normothermic controls. Our results suggest that the neuroprotective effect of mild hypothermia may be due, in part, to a reduction in neuronal and endothelial O−•2 production during early reperfusion.http://www.sciencedirect.com/science/article/pii/S0969996102905139hypothermiacerebral ischemiasuperoxide anionsuperoxide dismutasehydroethidinefree radicals |
| spellingShingle | Carolina M. Maier Guo Hua Sun Danye Cheng Midori A. Yenari Pak H. Chan Gary K. Steinberg Effects of Mild Hypothermia on Superoxide Anion Production, Superoxide Dismutase Expression, and Activity Following Transient Focal Cerebral Ischemia Neurobiology of Disease hypothermia cerebral ischemia superoxide anion superoxide dismutase hydroethidine free radicals |
| title | Effects of Mild Hypothermia on Superoxide Anion Production, Superoxide Dismutase Expression, and Activity Following Transient Focal Cerebral Ischemia |
| title_full | Effects of Mild Hypothermia on Superoxide Anion Production, Superoxide Dismutase Expression, and Activity Following Transient Focal Cerebral Ischemia |
| title_fullStr | Effects of Mild Hypothermia on Superoxide Anion Production, Superoxide Dismutase Expression, and Activity Following Transient Focal Cerebral Ischemia |
| title_full_unstemmed | Effects of Mild Hypothermia on Superoxide Anion Production, Superoxide Dismutase Expression, and Activity Following Transient Focal Cerebral Ischemia |
| title_short | Effects of Mild Hypothermia on Superoxide Anion Production, Superoxide Dismutase Expression, and Activity Following Transient Focal Cerebral Ischemia |
| title_sort | effects of mild hypothermia on superoxide anion production superoxide dismutase expression and activity following transient focal cerebral ischemia |
| topic | hypothermia cerebral ischemia superoxide anion superoxide dismutase hydroethidine free radicals |
| url | http://www.sciencedirect.com/science/article/pii/S0969996102905139 |
| work_keys_str_mv | AT carolinammaier effectsofmildhypothermiaonsuperoxideanionproductionsuperoxidedismutaseexpressionandactivityfollowingtransientfocalcerebralischemia AT guohuasun effectsofmildhypothermiaonsuperoxideanionproductionsuperoxidedismutaseexpressionandactivityfollowingtransientfocalcerebralischemia AT danyecheng effectsofmildhypothermiaonsuperoxideanionproductionsuperoxidedismutaseexpressionandactivityfollowingtransientfocalcerebralischemia AT midoriayenari effectsofmildhypothermiaonsuperoxideanionproductionsuperoxidedismutaseexpressionandactivityfollowingtransientfocalcerebralischemia AT pakhchan effectsofmildhypothermiaonsuperoxideanionproductionsuperoxidedismutaseexpressionandactivityfollowingtransientfocalcerebralischemia AT garyksteinberg effectsofmildhypothermiaonsuperoxideanionproductionsuperoxidedismutaseexpressionandactivityfollowingtransientfocalcerebralischemia |