Ex situ seed baiting to isolate germination-enhancing fungi for assisted colonization in Paphiopedilum spicerianum, a critically endangered orchid in China
Using orchid mycorrhizal fungi to facilitate orchid recovery projects is considered to be an effective method for orchid conservation. In this study, we used ex situ seed baiting to isolate fungi to assist seed germination in Paphiopedilum spicerianum, a critically endangered orchid with only a sing...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Global Ecology and Conservation |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2351989420306880 |
| _version_ | 1819110718978392064 |
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| author | Wen-Ke Yang Tai-Qiang Li Shi-Mao Wu Patrick M. Finnegan Jiang-Yun Gao |
| author_facet | Wen-Ke Yang Tai-Qiang Li Shi-Mao Wu Patrick M. Finnegan Jiang-Yun Gao |
| author_sort | Wen-Ke Yang |
| collection | DOAJ |
| description | Using orchid mycorrhizal fungi to facilitate orchid recovery projects is considered to be an effective method for orchid conservation. In this study, we used ex situ seed baiting to isolate fungi to assist seed germination in Paphiopedilum spicerianum, a critically endangered orchid with only a single population found in south China. Two fungal species, GYBQ01 and GYBQ02, were successfully obtained and identified as belonging to Tulasnellaceae. Both fungi were capable of promoting seed germination up to the seedling stage, as the percentage of seedlings in GYBQ01 and GYBQ02 treatments reached 34.9 ± 2.7% and 50.8 ± 3.5%, respectively, at 120 days after incubation. Both fungi also showed a strong host-specific relationship with P. spicerianum. Using these fungi, we developed fungi-seed bags to be used as propagules for assisted colonization of P. spicerianum. This approach had many advantages in practice, e.g., low-cost mass production, long term storage, convenient transportation, controllable seedling quantity and density, ease of use in the field, and environmently-friendly biodegradable paper bags. This is the first time that this approach has been applied to assisted colonization of an endangered orchid, and has universal application to other orchids. Our results have potential to provide a complete solution for orchid reintroduction, as well as assisted colonization, based on symbiotic seed germination. |
| first_indexed | 2024-12-22T03:46:11Z |
| format | Article |
| id | doaj.art-1aba64ca522d48baa2c228e7bffbe8f2 |
| institution | Directory Open Access Journal |
| issn | 2351-9894 |
| language | English |
| last_indexed | 2024-12-22T03:46:11Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Global Ecology and Conservation |
| spelling | doaj.art-1aba64ca522d48baa2c228e7bffbe8f22022-12-21T18:40:07ZengElsevierGlobal Ecology and Conservation2351-98942020-09-0123e01147Ex situ seed baiting to isolate germination-enhancing fungi for assisted colonization in Paphiopedilum spicerianum, a critically endangered orchid in ChinaWen-Ke Yang0Tai-Qiang Li1Shi-Mao Wu2Patrick M. Finnegan3Jiang-Yun Gao4Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology, Kunming 650500, China; Laboratory of Ecology and Evolutionary Biology, Yunnan University, Kunming 650500, ChinaYunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology, Kunming 650500, China; Laboratory of Ecology and Evolutionary Biology, Yunnan University, Kunming 650500, ChinaYunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology, Kunming 650500, China; Laboratory of Ecology and Evolutionary Biology, Yunnan University, Kunming 650500, ChinaSchool of Biological Sciences, University of Western Australia, Perth, WA, 6009, AustraliaYunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology, Kunming 650500, China; Laboratory of Ecology and Evolutionary Biology, Yunnan University, Kunming 650500, China; Corresponding author. Laboratory of Ecology and Evolutionary Biology, Yunnan University, Kunming 650500, China.Using orchid mycorrhizal fungi to facilitate orchid recovery projects is considered to be an effective method for orchid conservation. In this study, we used ex situ seed baiting to isolate fungi to assist seed germination in Paphiopedilum spicerianum, a critically endangered orchid with only a single population found in south China. Two fungal species, GYBQ01 and GYBQ02, were successfully obtained and identified as belonging to Tulasnellaceae. Both fungi were capable of promoting seed germination up to the seedling stage, as the percentage of seedlings in GYBQ01 and GYBQ02 treatments reached 34.9 ± 2.7% and 50.8 ± 3.5%, respectively, at 120 days after incubation. Both fungi also showed a strong host-specific relationship with P. spicerianum. Using these fungi, we developed fungi-seed bags to be used as propagules for assisted colonization of P. spicerianum. This approach had many advantages in practice, e.g., low-cost mass production, long term storage, convenient transportation, controllable seedling quantity and density, ease of use in the field, and environmently-friendly biodegradable paper bags. This is the first time that this approach has been applied to assisted colonization of an endangered orchid, and has universal application to other orchids. Our results have potential to provide a complete solution for orchid reintroduction, as well as assisted colonization, based on symbiotic seed germination.http://www.sciencedirect.com/science/article/pii/S2351989420306880Fungi-seed bagOrchid conservationOrchid mycorrhiza fungiPSESP in ChinaSymbiotic seed germinationTulasnella |
| spellingShingle | Wen-Ke Yang Tai-Qiang Li Shi-Mao Wu Patrick M. Finnegan Jiang-Yun Gao Ex situ seed baiting to isolate germination-enhancing fungi for assisted colonization in Paphiopedilum spicerianum, a critically endangered orchid in China Global Ecology and Conservation Fungi-seed bag Orchid conservation Orchid mycorrhiza fungi PSESP in China Symbiotic seed germination Tulasnella |
| title | Ex situ seed baiting to isolate germination-enhancing fungi for assisted colonization in Paphiopedilum spicerianum, a critically endangered orchid in China |
| title_full | Ex situ seed baiting to isolate germination-enhancing fungi for assisted colonization in Paphiopedilum spicerianum, a critically endangered orchid in China |
| title_fullStr | Ex situ seed baiting to isolate germination-enhancing fungi for assisted colonization in Paphiopedilum spicerianum, a critically endangered orchid in China |
| title_full_unstemmed | Ex situ seed baiting to isolate germination-enhancing fungi for assisted colonization in Paphiopedilum spicerianum, a critically endangered orchid in China |
| title_short | Ex situ seed baiting to isolate germination-enhancing fungi for assisted colonization in Paphiopedilum spicerianum, a critically endangered orchid in China |
| title_sort | ex situ seed baiting to isolate germination enhancing fungi for assisted colonization in paphiopedilum spicerianum a critically endangered orchid in china |
| topic | Fungi-seed bag Orchid conservation Orchid mycorrhiza fungi PSESP in China Symbiotic seed germination Tulasnella |
| url | http://www.sciencedirect.com/science/article/pii/S2351989420306880 |
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