Effects of maleimide-polyethylene glycol-modified human hemoglobin (MP4) on tissue necrosis in SKH1-hr hairless mice
<p>Abstract</p> <p>Objective</p> <p>Tissue hypoxia after blood loss, replantation and flap reperfusion remains a challenging task in surgery. Normovolemic hemodilution improves hemorheologic properties without increasing oxygen carrying capacity. Red blood cell transfus...
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| Format: | Article |
| Language: | English |
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BMC
2009-03-01
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| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | http://www.eurjmedres.com/content/14/3/123 |
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| author | Goertz O Kirschner MH Lilienfein H Babilas P Steinau HU Andree C Daigeler A Stachon A Homann H Langer S |
| author_facet | Goertz O Kirschner MH Lilienfein H Babilas P Steinau HU Andree C Daigeler A Stachon A Homann H Langer S |
| author_sort | Goertz O |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Objective</p> <p>Tissue hypoxia after blood loss, replantation and flap reperfusion remains a challenging task in surgery. Normovolemic hemodilution improves hemorheologic properties without increasing oxygen carrying capacity. Red blood cell transfusion is the current standard of treatment with its attendant risks. The aim of this study was to investigate the potential of the chemically modified hemoglobin, MP4, to reduce skin flap necrosis and its effect on selected blood markers and kidneys.</p> <p>Materials and methods</p> <p>Tissue ischemia was induced in the ear of hairless mice (n = 26). Hemodilution was performed by replacing one third of blood volume with the similar amount of MP4, dextran, or blood. The extent of non-perfused tissue was assessed by intravital fluorescent microscopy.</p> <p>Results</p> <p>Of all groups, MP4 showed the smallest area of no perfusion (in percentage of the ear ± SEM: 16.3% ± 2.4), the control group the largest (22.4% ± 3.5). Leukocytes showed a significant increase in the MP4 and dextran group (from 8.7 to 13.6 respectively 15.4*10<sup>9</sup>/l). On histology no changes of the kidneys could be observed.</p> <p>Conclusion</p> <p>MP4 causes an increase of leukocytes, improves the oxygen supply of the tissue and shows no evidence of renal impairment.</p> |
| first_indexed | 2024-12-18T06:03:25Z |
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| id | doaj.art-1aba6d90663c481eae774828d28a08c5 |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-12-18T06:03:25Z |
| publishDate | 2009-03-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-1aba6d90663c481eae774828d28a08c52022-12-21T21:18:37ZengBMCEuropean Journal of Medical Research2047-783X2009-03-0114312310.1186/2047-783X-14-3-123Effects of maleimide-polyethylene glycol-modified human hemoglobin (MP4) on tissue necrosis in SKH1-hr hairless miceGoertz OKirschner MHLilienfein HBabilas PSteinau HUAndree CDaigeler AStachon AHomann HLanger S<p>Abstract</p> <p>Objective</p> <p>Tissue hypoxia after blood loss, replantation and flap reperfusion remains a challenging task in surgery. Normovolemic hemodilution improves hemorheologic properties without increasing oxygen carrying capacity. Red blood cell transfusion is the current standard of treatment with its attendant risks. The aim of this study was to investigate the potential of the chemically modified hemoglobin, MP4, to reduce skin flap necrosis and its effect on selected blood markers and kidneys.</p> <p>Materials and methods</p> <p>Tissue ischemia was induced in the ear of hairless mice (n = 26). Hemodilution was performed by replacing one third of blood volume with the similar amount of MP4, dextran, or blood. The extent of non-perfused tissue was assessed by intravital fluorescent microscopy.</p> <p>Results</p> <p>Of all groups, MP4 showed the smallest area of no perfusion (in percentage of the ear ± SEM: 16.3% ± 2.4), the control group the largest (22.4% ± 3.5). Leukocytes showed a significant increase in the MP4 and dextran group (from 8.7 to 13.6 respectively 15.4*10<sup>9</sup>/l). On histology no changes of the kidneys could be observed.</p> <p>Conclusion</p> <p>MP4 causes an increase of leukocytes, improves the oxygen supply of the tissue and shows no evidence of renal impairment.</p>http://www.eurjmedres.com/content/14/3/123MP4hairless miceintravital fluorescent microscopyoxygen carrierfree hemoglobinintracardiac accesshemodilution |
| spellingShingle | Goertz O Kirschner MH Lilienfein H Babilas P Steinau HU Andree C Daigeler A Stachon A Homann H Langer S Effects of maleimide-polyethylene glycol-modified human hemoglobin (MP4) on tissue necrosis in SKH1-hr hairless mice European Journal of Medical Research MP4 hairless mice intravital fluorescent microscopy oxygen carrier free hemoglobin intracardiac access hemodilution |
| title | Effects of maleimide-polyethylene glycol-modified human hemoglobin (MP4) on tissue necrosis in SKH1-hr hairless mice |
| title_full | Effects of maleimide-polyethylene glycol-modified human hemoglobin (MP4) on tissue necrosis in SKH1-hr hairless mice |
| title_fullStr | Effects of maleimide-polyethylene glycol-modified human hemoglobin (MP4) on tissue necrosis in SKH1-hr hairless mice |
| title_full_unstemmed | Effects of maleimide-polyethylene glycol-modified human hemoglobin (MP4) on tissue necrosis in SKH1-hr hairless mice |
| title_short | Effects of maleimide-polyethylene glycol-modified human hemoglobin (MP4) on tissue necrosis in SKH1-hr hairless mice |
| title_sort | effects of maleimide polyethylene glycol modified human hemoglobin mp4 on tissue necrosis in skh1 hr hairless mice |
| topic | MP4 hairless mice intravital fluorescent microscopy oxygen carrier free hemoglobin intracardiac access hemodilution |
| url | http://www.eurjmedres.com/content/14/3/123 |
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