Draft genome sequence analysis of a mcr-1-producing Klebsiella pneumoniae ST661 isolated from a mink in China
ABSTRACT: Objectives: Klebsiella pneumoniae is a major opportunistic pathogen that is a member of the Enterobacteriaceae. Klebsiella pneumoniae causes pneumonia in mink and has become the primary infectious disease that limits mink farming. In this study, we report the draft genome sequence of a mu...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524000341 |
| _version_ | 1797235517294641152 |
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| author | Na Sun Yilin Luo Yaru Zhao Guisheng Wang Li Guo Liming Liu Zhihao San Cuiqing Zhao Yuening Cheng Qiang Chen |
| author_facet | Na Sun Yilin Luo Yaru Zhao Guisheng Wang Li Guo Liming Liu Zhihao San Cuiqing Zhao Yuening Cheng Qiang Chen |
| author_sort | Na Sun |
| collection | DOAJ |
| description | ABSTRACT: Objectives: Klebsiella pneumoniae is a major opportunistic pathogen that is a member of the Enterobacteriaceae. Klebsiella pneumoniae causes pneumonia in mink and has become the primary infectious disease that limits mink farming. In this study, we report the draft genome sequence of a multidrug-resistant (MDR) strain of K. pneumoniae that harbours the mcr-1 gene isolated from a mink in China. Methods: The agar microdilution method was used to determine the minimum inhibitory concentration of the strain. The entire genomic DNA was sequenced using an Illumina MiSeq platform. A multilocus sequence type (MLST) and a core genome SNP phylogenetic tree analysis with a heatmap of the resistance genes and virulence genes were performed. Results: The size of the genome was 5451.826 kb, and it included one chromosome and one plasmid. The draft genome of K. pneumoniae indicated that the isolate was a member of MLST 661. Four types of virulence genes were detected. The results of antimicrobial susceptibility testing showed multiple drug resistance, and 17 resistance genes were identified. Conclusion: The genome sequence reported in this study will help to reveal the key role of antibiotic resistance and pathogenic mechanisms. It will provide useful information for the role of mobile genetic elements in the adaptive translocation and spread of antimicrobial resistance. |
| first_indexed | 2024-04-24T16:49:13Z |
| format | Article |
| id | doaj.art-1abaae2d6e5e451bb1e998cff942bf94 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-04-24T16:49:13Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-1abaae2d6e5e451bb1e998cff942bf942024-03-29T05:50:17ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-06-01377274Draft genome sequence analysis of a mcr-1-producing Klebsiella pneumoniae ST661 isolated from a mink in ChinaNa Sun0Yilin Luo1Yaru Zhao2Guisheng Wang3Li Guo4Liming Liu5Zhihao San6Cuiqing Zhao7Yuening Cheng8Qiang Chen9College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin ChinaShandong Provincial Center for Animal Disease Control and Prevention, Jinan, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin ChinaKey Laboratory of Special Animal Epidemic Disease, Ministry of Agriculture, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin China; Corresponding author.ABSTRACT: Objectives: Klebsiella pneumoniae is a major opportunistic pathogen that is a member of the Enterobacteriaceae. Klebsiella pneumoniae causes pneumonia in mink and has become the primary infectious disease that limits mink farming. In this study, we report the draft genome sequence of a multidrug-resistant (MDR) strain of K. pneumoniae that harbours the mcr-1 gene isolated from a mink in China. Methods: The agar microdilution method was used to determine the minimum inhibitory concentration of the strain. The entire genomic DNA was sequenced using an Illumina MiSeq platform. A multilocus sequence type (MLST) and a core genome SNP phylogenetic tree analysis with a heatmap of the resistance genes and virulence genes were performed. Results: The size of the genome was 5451.826 kb, and it included one chromosome and one plasmid. The draft genome of K. pneumoniae indicated that the isolate was a member of MLST 661. Four types of virulence genes were detected. The results of antimicrobial susceptibility testing showed multiple drug resistance, and 17 resistance genes were identified. Conclusion: The genome sequence reported in this study will help to reveal the key role of antibiotic resistance and pathogenic mechanisms. It will provide useful information for the role of mobile genetic elements in the adaptive translocation and spread of antimicrobial resistance.http://www.sciencedirect.com/science/article/pii/S2213716524000341Klebsiella pneumoniaeMinkmcr-1Multidrug resistance |
| spellingShingle | Na Sun Yilin Luo Yaru Zhao Guisheng Wang Li Guo Liming Liu Zhihao San Cuiqing Zhao Yuening Cheng Qiang Chen Draft genome sequence analysis of a mcr-1-producing Klebsiella pneumoniae ST661 isolated from a mink in China Journal of Global Antimicrobial Resistance Klebsiella pneumoniae Mink mcr-1 Multidrug resistance |
| title | Draft genome sequence analysis of a mcr-1-producing Klebsiella pneumoniae ST661 isolated from a mink in China |
| title_full | Draft genome sequence analysis of a mcr-1-producing Klebsiella pneumoniae ST661 isolated from a mink in China |
| title_fullStr | Draft genome sequence analysis of a mcr-1-producing Klebsiella pneumoniae ST661 isolated from a mink in China |
| title_full_unstemmed | Draft genome sequence analysis of a mcr-1-producing Klebsiella pneumoniae ST661 isolated from a mink in China |
| title_short | Draft genome sequence analysis of a mcr-1-producing Klebsiella pneumoniae ST661 isolated from a mink in China |
| title_sort | draft genome sequence analysis of a mcr 1 producing klebsiella pneumoniae st661 isolated from a mink in china |
| topic | Klebsiella pneumoniae Mink mcr-1 Multidrug resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524000341 |
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