Qualitative study on diabetic cutaneous wound healing with radiation crosslinked bilayer collagen scaffold in rat model
Abstract Diabetes may leave patients more prone to skin problems, and minor skin conditions can more easily turn into serious damage to the extracellular matrix, which further impairs the skin's mechanical properties and delays wound healing. Therefore, the aim of the work is to develop extrace...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-33372-z |
| _version_ | 1797841058573320192 |
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| author | Hongwei Li Xin Chen Kang Ren Lihao Wu Gong Chen Ling Xu |
| author_facet | Hongwei Li Xin Chen Kang Ren Lihao Wu Gong Chen Ling Xu |
| author_sort | Hongwei Li |
| collection | DOAJ |
| description | Abstract Diabetes may leave patients more prone to skin problems, and minor skin conditions can more easily turn into serious damage to the extracellular matrix, which further impairs the skin's mechanical properties and delays wound healing. Therefore, the aim of the work is to develop extracellular matrix substitution to remodel the mechanical properties of diabetic cutaneous wound and thus accelerate diabetic wound healing. A green fabrication approach was used to prepare radiation crosslinked bilayer collagen scaffold from collagen dispersion. The morphological, mechanical and swelling characteristics of radiation crosslinked bilayer collagen scaffold were assessed to be suitable for cutaneous wound remodeling. The feasibility of radiation crosslinked bilayer collagen scaffold was performed on full-skin defect of streptozotocin-induced diabetic rats. The tissue specimens were harvested after 7, 14, and 21 days. Histopathological analysis showed that radiation crosslinked bilayer collagen scaffold has beneficial effects on inducing skin regeneration and remodeling in diabetic rats. In addition, immunohistochemical staining further revealed that the radiation crosslinked bilayer collagen scaffold could not only significantly accelerate the diabetic wound healing, but also promote angiogenesis factor (CD31) production. Vascularization was observed as early as day 7. The work expands the therapeutic ideas for cutaneous wound healing in diabetes. |
| first_indexed | 2024-04-09T16:24:50Z |
| format | Article |
| id | doaj.art-1abb5952a72f4fd8b86cbfc01f6f39d8 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T16:24:50Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1abb5952a72f4fd8b86cbfc01f6f39d82023-04-23T11:15:17ZengNature PortfolioScientific Reports2045-23222023-04-0113111110.1038/s41598-023-33372-zQualitative study on diabetic cutaneous wound healing with radiation crosslinked bilayer collagen scaffold in rat modelHongwei Li0Xin Chen1Kang Ren2Lihao Wu3Gong Chen4Ling Xu5State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Department of Laboratory Medicine, School of Public Heath, Xiamen UniversityDepartment of Burn, Beijing Jishuitan HospitalState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Department of Laboratory Medicine, School of Public Heath, Xiamen UniversityState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Department of Laboratory Medicine, School of Public Heath, Xiamen UniversityState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Department of Laboratory Medicine, School of Public Heath, Xiamen UniversityState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Department of Laboratory Medicine, School of Public Heath, Xiamen UniversityAbstract Diabetes may leave patients more prone to skin problems, and minor skin conditions can more easily turn into serious damage to the extracellular matrix, which further impairs the skin's mechanical properties and delays wound healing. Therefore, the aim of the work is to develop extracellular matrix substitution to remodel the mechanical properties of diabetic cutaneous wound and thus accelerate diabetic wound healing. A green fabrication approach was used to prepare radiation crosslinked bilayer collagen scaffold from collagen dispersion. The morphological, mechanical and swelling characteristics of radiation crosslinked bilayer collagen scaffold were assessed to be suitable for cutaneous wound remodeling. The feasibility of radiation crosslinked bilayer collagen scaffold was performed on full-skin defect of streptozotocin-induced diabetic rats. The tissue specimens were harvested after 7, 14, and 21 days. Histopathological analysis showed that radiation crosslinked bilayer collagen scaffold has beneficial effects on inducing skin regeneration and remodeling in diabetic rats. In addition, immunohistochemical staining further revealed that the radiation crosslinked bilayer collagen scaffold could not only significantly accelerate the diabetic wound healing, but also promote angiogenesis factor (CD31) production. Vascularization was observed as early as day 7. The work expands the therapeutic ideas for cutaneous wound healing in diabetes.https://doi.org/10.1038/s41598-023-33372-z |
| spellingShingle | Hongwei Li Xin Chen Kang Ren Lihao Wu Gong Chen Ling Xu Qualitative study on diabetic cutaneous wound healing with radiation crosslinked bilayer collagen scaffold in rat model Scientific Reports |
| title | Qualitative study on diabetic cutaneous wound healing with radiation crosslinked bilayer collagen scaffold in rat model |
| title_full | Qualitative study on diabetic cutaneous wound healing with radiation crosslinked bilayer collagen scaffold in rat model |
| title_fullStr | Qualitative study on diabetic cutaneous wound healing with radiation crosslinked bilayer collagen scaffold in rat model |
| title_full_unstemmed | Qualitative study on diabetic cutaneous wound healing with radiation crosslinked bilayer collagen scaffold in rat model |
| title_short | Qualitative study on diabetic cutaneous wound healing with radiation crosslinked bilayer collagen scaffold in rat model |
| title_sort | qualitative study on diabetic cutaneous wound healing with radiation crosslinked bilayer collagen scaffold in rat model |
| url | https://doi.org/10.1038/s41598-023-33372-z |
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