Evaluation of Alpha-Amylase Inhibitory, Antioxidant, and Antimicrobial Potential and Phytochemical Contents of <i>Polygonum hydropiper</i> L.
<i>Polygonum hydropiper</i> L. is a traditionally used medicinal plant. The present study was designed to explore the α-amylase inhibitory, antioxidant, and antimicrobial activities of <i>Polygonum hydropiper</i> L. Polarity-based solvent extracts (<i>n</i>-hexane...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/7/852 |
| _version_ | 1797563204260331520 |
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| author | Abdul Nasir Mushtaq Khan Zainab Rehman Atif Ali Khan Khalil Saira Farman Naeema Begum Muhammad Irfan Wasim Sajjad Zahida Parveen |
| author_facet | Abdul Nasir Mushtaq Khan Zainab Rehman Atif Ali Khan Khalil Saira Farman Naeema Begum Muhammad Irfan Wasim Sajjad Zahida Parveen |
| author_sort | Abdul Nasir |
| collection | DOAJ |
| description | <i>Polygonum hydropiper</i> L. is a traditionally used medicinal plant. The present study was designed to explore the α-amylase inhibitory, antioxidant, and antimicrobial activities of <i>Polygonum hydropiper</i> L. Polarity-based solvent extracts (<i>n</i>-hexane, acetone, chloroform, methanol, ethanol, and water) of <i>Polygonum hydropiper</i> leaves and stem were used. Antioxidant activity was assessed by free radical scavenging assay (FRAP) and 2,2-diphenylpicrylhydrazyl (DPPH) free radical scavenging activity methods. Quantitative phytochemical analyses suggested that the stem of <i>Polygonum hydropiper</i> L. contains higher levels of bioactive compounds than its leaves (<i>p</i> < 0.05). The results suggested that stem-derived extracts of <i>Polygonum hydropiper</i> L. are more active against bacterial species, including two Gram-positive and three Gram-negative strains. Moreover, our results showed that the bioactive compounds of <i>Polygonum hydropiper</i> L. significantly inhibit α-amylase activity. Finally, we reported the polarity-based solvent extracts of <i>Polygonum hydropiper</i> L. and revealed that the stem, rather than leaves, has a high antioxidant potential as measured by FRAP and DPPH assay with IC<sub>50</sub> values of 1.38 and 1.59 mg/mL, respectively. It may also be deducted from the data that the <i>Polygonum hydropiper</i> L. could be a significant candidate, which should be subjected to further isolation and characterization, to be used as an antidiabetic, antimicrobial and antioxidant resource in many industries, like food, pharmaceuticals and cosmetics. |
| first_indexed | 2024-03-10T18:39:11Z |
| format | Article |
| id | doaj.art-1ac000fee1784d6aaf3a6877d4f1e3c1 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T18:39:11Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-1ac000fee1784d6aaf3a6877d4f1e3c12023-11-20T05:58:25ZengMDPI AGPlants2223-77472020-07-019785210.3390/plants9070852Evaluation of Alpha-Amylase Inhibitory, Antioxidant, and Antimicrobial Potential and Phytochemical Contents of <i>Polygonum hydropiper</i> L.Abdul Nasir0Mushtaq Khan1Zainab Rehman2Atif Ali Khan Khalil3Saira Farman4Naeema Begum5Muhammad Irfan6Wasim Sajjad7Zahida Parveen8Department of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, PakistanDepartment of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, PakistanLaboratory of Animal and Human Physiology, Department of Animal Sciences, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Biological Sciences, National University of Medical Sciences, Rawalpindi 46000, PakistanDepartment of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, PakistanDepartment of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, PakistanCollege of Dentistry, Department of Oral Biology, University of Florida, Gainesville, FL 32610, USADepartment of Biological Sciences, National University of Medical Sciences, Rawalpindi 46000, PakistanDepartment of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan<i>Polygonum hydropiper</i> L. is a traditionally used medicinal plant. The present study was designed to explore the α-amylase inhibitory, antioxidant, and antimicrobial activities of <i>Polygonum hydropiper</i> L. Polarity-based solvent extracts (<i>n</i>-hexane, acetone, chloroform, methanol, ethanol, and water) of <i>Polygonum hydropiper</i> leaves and stem were used. Antioxidant activity was assessed by free radical scavenging assay (FRAP) and 2,2-diphenylpicrylhydrazyl (DPPH) free radical scavenging activity methods. Quantitative phytochemical analyses suggested that the stem of <i>Polygonum hydropiper</i> L. contains higher levels of bioactive compounds than its leaves (<i>p</i> < 0.05). The results suggested that stem-derived extracts of <i>Polygonum hydropiper</i> L. are more active against bacterial species, including two Gram-positive and three Gram-negative strains. Moreover, our results showed that the bioactive compounds of <i>Polygonum hydropiper</i> L. significantly inhibit α-amylase activity. Finally, we reported the polarity-based solvent extracts of <i>Polygonum hydropiper</i> L. and revealed that the stem, rather than leaves, has a high antioxidant potential as measured by FRAP and DPPH assay with IC<sub>50</sub> values of 1.38 and 1.59 mg/mL, respectively. It may also be deducted from the data that the <i>Polygonum hydropiper</i> L. could be a significant candidate, which should be subjected to further isolation and characterization, to be used as an antidiabetic, antimicrobial and antioxidant resource in many industries, like food, pharmaceuticals and cosmetics.https://www.mdpi.com/2223-7747/9/7/852water-pepperFRAPDPPHpolarity-based solvent extractionα-amylase inhibition |
| spellingShingle | Abdul Nasir Mushtaq Khan Zainab Rehman Atif Ali Khan Khalil Saira Farman Naeema Begum Muhammad Irfan Wasim Sajjad Zahida Parveen Evaluation of Alpha-Amylase Inhibitory, Antioxidant, and Antimicrobial Potential and Phytochemical Contents of <i>Polygonum hydropiper</i> L. Plants water-pepper FRAP DPPH polarity-based solvent extraction α-amylase inhibition |
| title | Evaluation of Alpha-Amylase Inhibitory, Antioxidant, and Antimicrobial Potential and Phytochemical Contents of <i>Polygonum hydropiper</i> L. |
| title_full | Evaluation of Alpha-Amylase Inhibitory, Antioxidant, and Antimicrobial Potential and Phytochemical Contents of <i>Polygonum hydropiper</i> L. |
| title_fullStr | Evaluation of Alpha-Amylase Inhibitory, Antioxidant, and Antimicrobial Potential and Phytochemical Contents of <i>Polygonum hydropiper</i> L. |
| title_full_unstemmed | Evaluation of Alpha-Amylase Inhibitory, Antioxidant, and Antimicrobial Potential and Phytochemical Contents of <i>Polygonum hydropiper</i> L. |
| title_short | Evaluation of Alpha-Amylase Inhibitory, Antioxidant, and Antimicrobial Potential and Phytochemical Contents of <i>Polygonum hydropiper</i> L. |
| title_sort | evaluation of alpha amylase inhibitory antioxidant and antimicrobial potential and phytochemical contents of i polygonum hydropiper i l |
| topic | water-pepper FRAP DPPH polarity-based solvent extraction α-amylase inhibition |
| url | https://www.mdpi.com/2223-7747/9/7/852 |
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