Transcriptome dataset of gall-rust infected Sengon (Falcataria falcata) seedlings using long-read PCR-cDNA sequencing
Sengon (Falcataria falcata) is an economically important legume tree widely cultivated in community forests, especially in Java Island. However, attacks of gall rust disease by Uromycladium falcatariae is difficult to manage. Understanding sengon genes expressions when artificially infected with gal...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340923009587 |
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| author | Aditya Nugroho Iskandar Zulkarnaen Siregar Deden Derajat Matra Ulfah Juniarti Siregar |
| author_facet | Aditya Nugroho Iskandar Zulkarnaen Siregar Deden Derajat Matra Ulfah Juniarti Siregar |
| author_sort | Aditya Nugroho |
| collection | DOAJ |
| description | Sengon (Falcataria falcata) is an economically important legume tree widely cultivated in community forests, especially in Java Island. However, attacks of gall rust disease by Uromycladium falcatariae is difficult to manage. Understanding sengon genes expressions when artificially infected with gall rust fungi can help unravel its resistance mechanisms. Total RNA was extracted from sengon seedlings samples inoculated with U. falcatariae fungi at 7, 21, and 35 days after inoculation (DAI) and from the control group. Total RNA sequencing was performed using the PCR-cDNA Sequencing protocol (SQK-PCB109) from Oxford Nanopore Technologies. The RNA-Seq obtained varies from 1.3 million to 1.9 million total reads. The assembled full-length transcript was constructed using the RATTLE program, resulting in 21,819 transcripts. The TransDecoder program used to define open reading frames (ORFs) generated 2,342 transcripts, of which 34.15% were 5′prime_partial, 8.15% were 3′prime_partial, 8.5% were internal, and 49.14% were complete. Analysis of differentially expressed genes (DEGs) between resistant and susceptible seedlings, found that 1,013 genes that were up-regulated and 1,130 genes that were down-regulated in the resistant lines. The transcriptome data discussed in this article have been deposited in the DDBJ with accession number DRA015681. |
| first_indexed | 2024-03-08T03:30:01Z |
| format | Article |
| id | doaj.art-1ac03643c95a4df386244cdcfc39bbc4 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-03-08T03:30:01Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-1ac03643c95a4df386244cdcfc39bbc42024-02-11T05:10:33ZengElsevierData in Brief2352-34092024-02-0152109919Transcriptome dataset of gall-rust infected Sengon (Falcataria falcata) seedlings using long-read PCR-cDNA sequencingAditya Nugroho0Iskandar Zulkarnaen Siregar1Deden Derajat Matra2Ulfah Juniarti Siregar3Tropical Silviculture Study Program, Graduate School of IPB University, Bogor, IndonesiaDepartment of Silviculture, Faculty of Forestry and Environment, IPB University, Bogor, IndonesiaDepartment of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, IndonesiaDepartment of Silviculture, Faculty of Forestry and Environment, IPB University, Bogor, Indonesia; Corresponding author.Sengon (Falcataria falcata) is an economically important legume tree widely cultivated in community forests, especially in Java Island. However, attacks of gall rust disease by Uromycladium falcatariae is difficult to manage. Understanding sengon genes expressions when artificially infected with gall rust fungi can help unravel its resistance mechanisms. Total RNA was extracted from sengon seedlings samples inoculated with U. falcatariae fungi at 7, 21, and 35 days after inoculation (DAI) and from the control group. Total RNA sequencing was performed using the PCR-cDNA Sequencing protocol (SQK-PCB109) from Oxford Nanopore Technologies. The RNA-Seq obtained varies from 1.3 million to 1.9 million total reads. The assembled full-length transcript was constructed using the RATTLE program, resulting in 21,819 transcripts. The TransDecoder program used to define open reading frames (ORFs) generated 2,342 transcripts, of which 34.15% were 5′prime_partial, 8.15% were 3′prime_partial, 8.5% were internal, and 49.14% were complete. Analysis of differentially expressed genes (DEGs) between resistant and susceptible seedlings, found that 1,013 genes that were up-regulated and 1,130 genes that were down-regulated in the resistant lines. The transcriptome data discussed in this article have been deposited in the DDBJ with accession number DRA015681.http://www.sciencedirect.com/science/article/pii/S2352340923009587Long-read sequencingPlant defenseResistanceSengonUromycladium falcatariae |
| spellingShingle | Aditya Nugroho Iskandar Zulkarnaen Siregar Deden Derajat Matra Ulfah Juniarti Siregar Transcriptome dataset of gall-rust infected Sengon (Falcataria falcata) seedlings using long-read PCR-cDNA sequencing Data in Brief Long-read sequencing Plant defense Resistance Sengon Uromycladium falcatariae |
| title | Transcriptome dataset of gall-rust infected Sengon (Falcataria falcata) seedlings using long-read PCR-cDNA sequencing |
| title_full | Transcriptome dataset of gall-rust infected Sengon (Falcataria falcata) seedlings using long-read PCR-cDNA sequencing |
| title_fullStr | Transcriptome dataset of gall-rust infected Sengon (Falcataria falcata) seedlings using long-read PCR-cDNA sequencing |
| title_full_unstemmed | Transcriptome dataset of gall-rust infected Sengon (Falcataria falcata) seedlings using long-read PCR-cDNA sequencing |
| title_short | Transcriptome dataset of gall-rust infected Sengon (Falcataria falcata) seedlings using long-read PCR-cDNA sequencing |
| title_sort | transcriptome dataset of gall rust infected sengon falcataria falcata seedlings using long read pcr cdna sequencing |
| topic | Long-read sequencing Plant defense Resistance Sengon Uromycladium falcatariae |
| url | http://www.sciencedirect.com/science/article/pii/S2352340923009587 |
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