A novel method to extend viability and functionality of living heart slices
Living heart slices have recently emerged as a powerful experimental model for fundamental cardiac research. By retaining the structure and function of the native myocardium while maintaining the simplicity of cell culture models, heart slices can be easily employed in electrophysiological, pharmaco...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Cardiovascular Medicine |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2023.1244630/full |
| _version_ | 1797662845582704640 |
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| author | Abigail J. Ross Iva Krumova Berfin Tunc Qin Wu Qin Wu Changhao Wu Patrizia Camelliti |
| author_facet | Abigail J. Ross Iva Krumova Berfin Tunc Qin Wu Qin Wu Changhao Wu Patrizia Camelliti |
| author_sort | Abigail J. Ross |
| collection | DOAJ |
| description | Living heart slices have recently emerged as a powerful experimental model for fundamental cardiac research. By retaining the structure and function of the native myocardium while maintaining the simplicity of cell culture models, heart slices can be easily employed in electrophysiological, pharmacological, biochemical, and structural investigations. One single heart yields many slices (>20 slices for rodents, >100 slices for porcine or human hearts), however due to the low throughput of most assays and rapid slice degeneration within 24 h of preparation, many slices remain unused and are discarded at the end of the preparation day. Here we present a novel method to extend viability and functionality of living heart slices, enabling their use in experiments over several consecutive days following preparation. By combining hypothermic conditions with inhibition of myosin II ATPase using 2,3-butanedione monoxime (BDM), slices prepared from the left ventricle of porcine hearts remain viable and exhibit preserved contractile function and morphology for up to 6 days. Electrophysiological function was also confirmed over the 6 days by extracellular field potentials recordings. This simple method not only maximizes the use of slices prepared from one single heart, thus reducing the number of animals required, but also increases data reproducibility by allowing multiple electrophysiological, pharmacological, biochemical, and structural studies to be performed from the same heart. |
| first_indexed | 2024-03-11T19:06:01Z |
| format | Article |
| id | doaj.art-1ac636cf22f64001a5c033c41252f2c9 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-03-11T19:06:01Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-1ac636cf22f64001a5c033c41252f2c92023-10-10T07:16:31ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2023-10-011010.3389/fcvm.2023.12446301244630A novel method to extend viability and functionality of living heart slicesAbigail J. Ross0Iva Krumova1Berfin Tunc2Qin Wu3Qin Wu4Changhao Wu5Patrizia Camelliti6School of Biosciences and Medicine, University of Surrey, Guildford, United KingdomSchool of Biosciences and Medicine, University of Surrey, Guildford, United KingdomSchool of Biosciences and Medicine, University of Surrey, Guildford, United KingdomSchool of Biosciences and Medicine, University of Surrey, Guildford, United KingdomSchool of Medicine, Jiangsu Vocational College of Medicine, Yancheng, ChinaSchool of Biosciences and Medicine, University of Surrey, Guildford, United KingdomSchool of Biosciences and Medicine, University of Surrey, Guildford, United KingdomLiving heart slices have recently emerged as a powerful experimental model for fundamental cardiac research. By retaining the structure and function of the native myocardium while maintaining the simplicity of cell culture models, heart slices can be easily employed in electrophysiological, pharmacological, biochemical, and structural investigations. One single heart yields many slices (>20 slices for rodents, >100 slices for porcine or human hearts), however due to the low throughput of most assays and rapid slice degeneration within 24 h of preparation, many slices remain unused and are discarded at the end of the preparation day. Here we present a novel method to extend viability and functionality of living heart slices, enabling their use in experiments over several consecutive days following preparation. By combining hypothermic conditions with inhibition of myosin II ATPase using 2,3-butanedione monoxime (BDM), slices prepared from the left ventricle of porcine hearts remain viable and exhibit preserved contractile function and morphology for up to 6 days. Electrophysiological function was also confirmed over the 6 days by extracellular field potentials recordings. This simple method not only maximizes the use of slices prepared from one single heart, thus reducing the number of animals required, but also increases data reproducibility by allowing multiple electrophysiological, pharmacological, biochemical, and structural studies to be performed from the same heart.https://www.frontiersin.org/articles/10.3389/fcvm.2023.1244630/fullmyocardial slicesorganotypic ex-vivo modelsBDM (2,3-Butanedione monoxime)myosin II ATPasecontractilityfield potential |
| spellingShingle | Abigail J. Ross Iva Krumova Berfin Tunc Qin Wu Qin Wu Changhao Wu Patrizia Camelliti A novel method to extend viability and functionality of living heart slices Frontiers in Cardiovascular Medicine myocardial slices organotypic ex-vivo models BDM (2,3-Butanedione monoxime) myosin II ATPase contractility field potential |
| title | A novel method to extend viability and functionality of living heart slices |
| title_full | A novel method to extend viability and functionality of living heart slices |
| title_fullStr | A novel method to extend viability and functionality of living heart slices |
| title_full_unstemmed | A novel method to extend viability and functionality of living heart slices |
| title_short | A novel method to extend viability and functionality of living heart slices |
| title_sort | novel method to extend viability and functionality of living heart slices |
| topic | myocardial slices organotypic ex-vivo models BDM (2,3-Butanedione monoxime) myosin II ATPase contractility field potential |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2023.1244630/full |
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