The collaborative mode by PmSVPs and PmDAMs reveals neofunctionalization in the switch of the flower bud development and dormancy for Prunus mume
Prunus mume (Rosaceae, Prunoideae) serves as an excellent ornamental woody plant with a large-temperature-range cultivation scope. Its flower buds require a certain low temperature to achieve flowering circulation. Thus, it is important to delve into the processes of flower bud differentiation and d...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1023628/full |
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| author | Kai Zhao Yuzhen Zhou Yan Zheng Rui-yue Zheng Meijuan Hu Yan Tong Xianmei Luo Yangting Zhang Ming-li Shen |
| author_facet | Kai Zhao Yuzhen Zhou Yan Zheng Rui-yue Zheng Meijuan Hu Yan Tong Xianmei Luo Yangting Zhang Ming-li Shen |
| author_sort | Kai Zhao |
| collection | DOAJ |
| description | Prunus mume (Rosaceae, Prunoideae) serves as an excellent ornamental woody plant with a large-temperature-range cultivation scope. Its flower buds require a certain low temperature to achieve flowering circulation. Thus, it is important to delve into the processes of flower bud differentiation and dormancy, which affected its continuous flowering. These processes are generally considered as regulation by the MADS-box homologs, SHORT VEGETATIVE PHASE (SVP), and DORMANCY-ASSOCIATED MADS-BOX (DAM). However, a precise model on their interdependence and specific function, when acting as a complex in the flower development of P. mume, is needed. Therefore, this study highlighted the integral roles of PmDAMs and PmSVPs in flower organ development and dormancy cycle. The segregation of PmDAMs and PmSVPs in a different cluster suggested distinct functions and neofunctionalization. The expression pattern and yeast two-hybrid assays jointly revealed that eight genes were involved in the floral organ development stages, with PmDAM1 and PmDAM5 specifically related to prolificated flower formation. PmSVP1–2 mingled in the protein complex in bud dormancy stages with PmDAMs. Finally, we proposed the hypothesis that PmSVP1 and PmSVP2 could combine with PmDAM1 to have an effect on flower organogenesis and interact with PmDAM5 and PmDAM6 to regulate flower bud dormancy. These findings could help expand the current molecular mechanism based on MADS-box genes during flower bud development and dormancy. |
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| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T06:31:22Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-1acb0a0d581b412886d5a88ea027d9c32022-12-22T04:40:01ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-12-011310.3389/fpls.2022.10236281023628The collaborative mode by PmSVPs and PmDAMs reveals neofunctionalization in the switch of the flower bud development and dormancy for Prunus mumeKai Zhao0Yuzhen Zhou1Yan Zheng2Rui-yue Zheng3Meijuan Hu4Yan Tong5Xianmei Luo6Yangting Zhang7Ming-li Shen8College of Life Sciences, Fujian Normal University, Fuzhou, ChinaCollege of Landscape Architecture, Ornamental Plant Germplasm Resources Innovation and Engineering Application Research Center at College of Landscape Architecture, Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Landscape Architecture, Ornamental Plant Germplasm Resources Innovation and Engineering Application Research Center at College of Landscape Architecture, Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Landscape Architecture, Ornamental Plant Germplasm Resources Innovation and Engineering Application Research Center at College of Landscape Architecture, Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Landscape Architecture, Ornamental Plant Germplasm Resources Innovation and Engineering Application Research Center at College of Landscape Architecture, Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Landscape Architecture, Ornamental Plant Germplasm Resources Innovation and Engineering Application Research Center at College of Landscape Architecture, Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Life Sciences, Fujian Normal University, Fuzhou, ChinaCollege of Landscape Architecture, Ornamental Plant Germplasm Resources Innovation and Engineering Application Research Center at College of Landscape Architecture, Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Life Sciences, Fujian Normal University, Fuzhou, ChinaPrunus mume (Rosaceae, Prunoideae) serves as an excellent ornamental woody plant with a large-temperature-range cultivation scope. Its flower buds require a certain low temperature to achieve flowering circulation. Thus, it is important to delve into the processes of flower bud differentiation and dormancy, which affected its continuous flowering. These processes are generally considered as regulation by the MADS-box homologs, SHORT VEGETATIVE PHASE (SVP), and DORMANCY-ASSOCIATED MADS-BOX (DAM). However, a precise model on their interdependence and specific function, when acting as a complex in the flower development of P. mume, is needed. Therefore, this study highlighted the integral roles of PmDAMs and PmSVPs in flower organ development and dormancy cycle. The segregation of PmDAMs and PmSVPs in a different cluster suggested distinct functions and neofunctionalization. The expression pattern and yeast two-hybrid assays jointly revealed that eight genes were involved in the floral organ development stages, with PmDAM1 and PmDAM5 specifically related to prolificated flower formation. PmSVP1–2 mingled in the protein complex in bud dormancy stages with PmDAMs. Finally, we proposed the hypothesis that PmSVP1 and PmSVP2 could combine with PmDAM1 to have an effect on flower organogenesis and interact with PmDAM5 and PmDAM6 to regulate flower bud dormancy. These findings could help expand the current molecular mechanism based on MADS-box genes during flower bud development and dormancy.https://www.frontiersin.org/articles/10.3389/fpls.2022.1023628/fullPrunus mumeSVP geneDAM genefloral organ developmentdormancyyeast two-hybrid assays |
| spellingShingle | Kai Zhao Yuzhen Zhou Yan Zheng Rui-yue Zheng Meijuan Hu Yan Tong Xianmei Luo Yangting Zhang Ming-li Shen The collaborative mode by PmSVPs and PmDAMs reveals neofunctionalization in the switch of the flower bud development and dormancy for Prunus mume Frontiers in Plant Science Prunus mume SVP gene DAM gene floral organ development dormancy yeast two-hybrid assays |
| title | The collaborative mode by PmSVPs and PmDAMs reveals neofunctionalization in the switch of the flower bud development and dormancy for Prunus mume |
| title_full | The collaborative mode by PmSVPs and PmDAMs reveals neofunctionalization in the switch of the flower bud development and dormancy for Prunus mume |
| title_fullStr | The collaborative mode by PmSVPs and PmDAMs reveals neofunctionalization in the switch of the flower bud development and dormancy for Prunus mume |
| title_full_unstemmed | The collaborative mode by PmSVPs and PmDAMs reveals neofunctionalization in the switch of the flower bud development and dormancy for Prunus mume |
| title_short | The collaborative mode by PmSVPs and PmDAMs reveals neofunctionalization in the switch of the flower bud development and dormancy for Prunus mume |
| title_sort | collaborative mode by pmsvps and pmdams reveals neofunctionalization in the switch of the flower bud development and dormancy for prunus mume |
| topic | Prunus mume SVP gene DAM gene floral organ development dormancy yeast two-hybrid assays |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1023628/full |
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