Expression levels of novel cytokine IL-32 in periodontitis and its role in the suppression of IL-8 production by human gingival fibroblasts stimulated with Porphyromonas gingivalis
Background:IL-32 was recently found to be elevated in the tissue of rheumatoid arthritis and inflammatory bowel disease. Periodontitis is a chronic inflammatory disease caused by polymicrobial infections that result in soft tissue destruction and alveolar bone loss. Although IL-32 is also thought to...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2012-03-01
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| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://www.journaloforalmicrobiology.net/index.php/jom/article/view/14832/20850 |
| _version_ | 1818791728046407680 |
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| author | Kazuhisa Ouhara Toshihisa Kawai Marcelo J.B. Silva Tsuyoshi Fujita Kouichi Hayashida Nadeem Y. Karimbux Mikihito Kajiya Hideki Shiba Hiroyuki Kawaguchi Hidemi Kurihara |
| author_facet | Kazuhisa Ouhara Toshihisa Kawai Marcelo J.B. Silva Tsuyoshi Fujita Kouichi Hayashida Nadeem Y. Karimbux Mikihito Kajiya Hideki Shiba Hiroyuki Kawaguchi Hidemi Kurihara |
| author_sort | Kazuhisa Ouhara |
| collection | DOAJ |
| description | Background:IL-32 was recently found to be elevated in the tissue of rheumatoid arthritis and inflammatory bowel disease. Periodontitis is a chronic inflammatory disease caused by polymicrobial infections that result in soft tissue destruction and alveolar bone loss. Although IL-32 is also thought to be associated with periodontal disease, its expression and possible role in periodontal tissue remain unclear. Therefore, this study investigated the expression patterns of IL-32 in healthy and periodontally diseased gingival tissue. The expression of IL-32 in cultured human gingival fibroblasts (HGF) as well as effects of autocrine IL-32 on IL-8 production from HGF were also examined.Methods:Periodontal tissue was collected from both healthy volunteers and periodontitis patients, and immunofluorescent staining was performed in order to determine the production of IL-32. Using real-time PCR and ELISA, mRNA expression and protein production of IL-32 in HGF, stimulated by Porphyromonas gingivalis (Pg), were also investigated.Results:Contrary to our expectation, the production of IL-32 in the periodontitis patients was significantly lower than in the healthy volunteers. According to immunofluorescent microscopy, positive staining for IL-32 was detected in prickle and basal cell layers in the epithelium as well as fibroblastic cells in connective tissue. Addition of fixed Pg in vitro was found to suppress the otherwise constitutive expression of IL-32 mRNA and protein in HGF. However, recombinant IL-32 in vitro inhibited the expression of IL-8 mRNA by HGF stimulated with Pg. Interestingly, anti-IL-32 neutralizing antibody upregulated the IL-8 mRNA expression in non-stimulated HGF, indicating that constitutive expression of IL-32 in HGF suppressed IL-8 mRNA expression in the absence of bacterial stimulation.Conclusion:These results indicate that IL-32 is constitutively produced by HGF which can be suppressed by Pg and may play a role in the downregulation of inflammatory responses, such as IL-8 production, in periodontal tissue. |
| first_indexed | 2024-12-18T15:15:57Z |
| format | Article |
| id | doaj.art-1acb7f28b91e41f88985083bad37aecf |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-18T15:15:57Z |
| publishDate | 2012-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-1acb7f28b91e41f88985083bad37aecf2022-12-21T21:03:31ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972012-03-01401810.3402/jom.v4i0.14832Expression levels of novel cytokine IL-32 in periodontitis and its role in the suppression of IL-8 production by human gingival fibroblasts stimulated with Porphyromonas gingivalisKazuhisa OuharaToshihisa KawaiMarcelo J.B. SilvaTsuyoshi FujitaKouichi HayashidaNadeem Y. KarimbuxMikihito KajiyaHideki ShibaHiroyuki KawaguchiHidemi KuriharaBackground:IL-32 was recently found to be elevated in the tissue of rheumatoid arthritis and inflammatory bowel disease. Periodontitis is a chronic inflammatory disease caused by polymicrobial infections that result in soft tissue destruction and alveolar bone loss. Although IL-32 is also thought to be associated with periodontal disease, its expression and possible role in periodontal tissue remain unclear. Therefore, this study investigated the expression patterns of IL-32 in healthy and periodontally diseased gingival tissue. The expression of IL-32 in cultured human gingival fibroblasts (HGF) as well as effects of autocrine IL-32 on IL-8 production from HGF were also examined.Methods:Periodontal tissue was collected from both healthy volunteers and periodontitis patients, and immunofluorescent staining was performed in order to determine the production of IL-32. Using real-time PCR and ELISA, mRNA expression and protein production of IL-32 in HGF, stimulated by Porphyromonas gingivalis (Pg), were also investigated.Results:Contrary to our expectation, the production of IL-32 in the periodontitis patients was significantly lower than in the healthy volunteers. According to immunofluorescent microscopy, positive staining for IL-32 was detected in prickle and basal cell layers in the epithelium as well as fibroblastic cells in connective tissue. Addition of fixed Pg in vitro was found to suppress the otherwise constitutive expression of IL-32 mRNA and protein in HGF. However, recombinant IL-32 in vitro inhibited the expression of IL-8 mRNA by HGF stimulated with Pg. Interestingly, anti-IL-32 neutralizing antibody upregulated the IL-8 mRNA expression in non-stimulated HGF, indicating that constitutive expression of IL-32 in HGF suppressed IL-8 mRNA expression in the absence of bacterial stimulation.Conclusion:These results indicate that IL-32 is constitutively produced by HGF which can be suppressed by Pg and may play a role in the downregulation of inflammatory responses, such as IL-8 production, in periodontal tissue.http://www.journaloforalmicrobiology.net/index.php/jom/article/view/14832/20850Interleukin-32periodontal diseasePorphyromonas gingivalishuman gingival fibroblastInterleukin-8 |
| spellingShingle | Kazuhisa Ouhara Toshihisa Kawai Marcelo J.B. Silva Tsuyoshi Fujita Kouichi Hayashida Nadeem Y. Karimbux Mikihito Kajiya Hideki Shiba Hiroyuki Kawaguchi Hidemi Kurihara Expression levels of novel cytokine IL-32 in periodontitis and its role in the suppression of IL-8 production by human gingival fibroblasts stimulated with Porphyromonas gingivalis Journal of Oral Microbiology Interleukin-32 periodontal disease Porphyromonas gingivalis human gingival fibroblast Interleukin-8 |
| title | Expression levels of novel cytokine IL-32 in periodontitis and its role in the suppression of IL-8 production by human gingival fibroblasts stimulated with Porphyromonas gingivalis |
| title_full | Expression levels of novel cytokine IL-32 in periodontitis and its role in the suppression of IL-8 production by human gingival fibroblasts stimulated with Porphyromonas gingivalis |
| title_fullStr | Expression levels of novel cytokine IL-32 in periodontitis and its role in the suppression of IL-8 production by human gingival fibroblasts stimulated with Porphyromonas gingivalis |
| title_full_unstemmed | Expression levels of novel cytokine IL-32 in periodontitis and its role in the suppression of IL-8 production by human gingival fibroblasts stimulated with Porphyromonas gingivalis |
| title_short | Expression levels of novel cytokine IL-32 in periodontitis and its role in the suppression of IL-8 production by human gingival fibroblasts stimulated with Porphyromonas gingivalis |
| title_sort | expression levels of novel cytokine il 32 in periodontitis and its role in the suppression of il 8 production by human gingival fibroblasts stimulated with porphyromonas gingivalis |
| topic | Interleukin-32 periodontal disease Porphyromonas gingivalis human gingival fibroblast Interleukin-8 |
| url | http://www.journaloforalmicrobiology.net/index.php/jom/article/view/14832/20850 |
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