Stress induced <it>Salmonella </it>Typhimurium recrudescence in pigs coincides with cortisol induced increased intracellular proliferation in macrophages
<p>Abstract</p> <p><it>Salmonella </it>Typhimurium infections in pigs often result in the development of carriers that intermittently excrete <it>Salmonella </it>in very low numbers. During periods of stress, for example transport to the slaughterhouse, recr...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-12-01
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| Series: | Veterinary Research |
| Online Access: | http://www.veterinaryresearch.org/content/42/1/118 |
| _version_ | 1818579872094617600 |
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| author | Verbrugghe Elin Boyen Filip Van Parys Alexander Van Deun Kim Croubels Siska Thompson Arthur Shearer Neil Leyman Bregje Haesebrouck Freddy Pasmans Frank |
| author_facet | Verbrugghe Elin Boyen Filip Van Parys Alexander Van Deun Kim Croubels Siska Thompson Arthur Shearer Neil Leyman Bregje Haesebrouck Freddy Pasmans Frank |
| author_sort | Verbrugghe Elin |
| collection | DOAJ |
| description | <p>Abstract</p> <p><it>Salmonella </it>Typhimurium infections in pigs often result in the development of carriers that intermittently excrete <it>Salmonella </it>in very low numbers. During periods of stress, for example transport to the slaughterhouse, recrudescence of <it>Salmonella </it>may occur, but the mechanism of this stress related recrudescence is poorly understood. Therefore, the aim of the present study was to determine the role of the stress hormone cortisol in <it>Salmonella </it>recrudescence by pigs. We showed that a 24 h feed withdrawal increases the intestinal <it>Salmonella </it>Typhimurium load in pigs, which is correlated with increased serum cortisol levels. A second in vivo trial demonstrated that stress related recrudescence of <it>Salmonella </it>Typhimurium in pigs can be induced by intramuscular injection of dexamethasone. Furthermore, we found that cortisol, but not epinephrine, norepinephrine and dopamine, promotes intracellular proliferation of <it>Salmonella </it>Typhimurium in primary porcine alveolar macrophages, but not in intestinal epithelial cells and a transformed cell line of porcine alveolar macrophages. A microarray based transcriptomic analysis revealed that cortisol did not directly affect the growth or the gene expression or <it>Salmonella </it>Typhimurium in a rich medium, which implies that the enhanced intracellular proliferation of the bacterium is probably caused by an indirect effect through the cell. These results highlight the role of cortisol in the recrudescence of <it>Salmonella </it>Typhimurium by pigs and they provide new evidence for the role of microbial endocrinology in host-pathogen interactions.</p> |
| first_indexed | 2024-12-16T07:08:36Z |
| format | Article |
| id | doaj.art-1acd7ae42a2e4b4c94742a684062ded3 |
| institution | Directory Open Access Journal |
| issn | 0928-4249 1297-9716 |
| language | English |
| last_indexed | 2024-12-16T07:08:36Z |
| publishDate | 2011-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-1acd7ae42a2e4b4c94742a684062ded32022-12-21T22:39:57ZengBMCVeterinary Research0928-42491297-97162011-12-0142111810.1186/1297-9716-42-118Stress induced <it>Salmonella </it>Typhimurium recrudescence in pigs coincides with cortisol induced increased intracellular proliferation in macrophagesVerbrugghe ElinBoyen FilipVan Parys AlexanderVan Deun KimCroubels SiskaThompson ArthurShearer NeilLeyman BregjeHaesebrouck FreddyPasmans Frank<p>Abstract</p> <p><it>Salmonella </it>Typhimurium infections in pigs often result in the development of carriers that intermittently excrete <it>Salmonella </it>in very low numbers. During periods of stress, for example transport to the slaughterhouse, recrudescence of <it>Salmonella </it>may occur, but the mechanism of this stress related recrudescence is poorly understood. Therefore, the aim of the present study was to determine the role of the stress hormone cortisol in <it>Salmonella </it>recrudescence by pigs. We showed that a 24 h feed withdrawal increases the intestinal <it>Salmonella </it>Typhimurium load in pigs, which is correlated with increased serum cortisol levels. A second in vivo trial demonstrated that stress related recrudescence of <it>Salmonella </it>Typhimurium in pigs can be induced by intramuscular injection of dexamethasone. Furthermore, we found that cortisol, but not epinephrine, norepinephrine and dopamine, promotes intracellular proliferation of <it>Salmonella </it>Typhimurium in primary porcine alveolar macrophages, but not in intestinal epithelial cells and a transformed cell line of porcine alveolar macrophages. A microarray based transcriptomic analysis revealed that cortisol did not directly affect the growth or the gene expression or <it>Salmonella </it>Typhimurium in a rich medium, which implies that the enhanced intracellular proliferation of the bacterium is probably caused by an indirect effect through the cell. These results highlight the role of cortisol in the recrudescence of <it>Salmonella </it>Typhimurium by pigs and they provide new evidence for the role of microbial endocrinology in host-pathogen interactions.</p>http://www.veterinaryresearch.org/content/42/1/118 |
| spellingShingle | Verbrugghe Elin Boyen Filip Van Parys Alexander Van Deun Kim Croubels Siska Thompson Arthur Shearer Neil Leyman Bregje Haesebrouck Freddy Pasmans Frank Stress induced <it>Salmonella </it>Typhimurium recrudescence in pigs coincides with cortisol induced increased intracellular proliferation in macrophages Veterinary Research |
| title | Stress induced <it>Salmonella </it>Typhimurium recrudescence in pigs coincides with cortisol induced increased intracellular proliferation in macrophages |
| title_full | Stress induced <it>Salmonella </it>Typhimurium recrudescence in pigs coincides with cortisol induced increased intracellular proliferation in macrophages |
| title_fullStr | Stress induced <it>Salmonella </it>Typhimurium recrudescence in pigs coincides with cortisol induced increased intracellular proliferation in macrophages |
| title_full_unstemmed | Stress induced <it>Salmonella </it>Typhimurium recrudescence in pigs coincides with cortisol induced increased intracellular proliferation in macrophages |
| title_short | Stress induced <it>Salmonella </it>Typhimurium recrudescence in pigs coincides with cortisol induced increased intracellular proliferation in macrophages |
| title_sort | stress induced it salmonella it typhimurium recrudescence in pigs coincides with cortisol induced increased intracellular proliferation in macrophages |
| url | http://www.veterinaryresearch.org/content/42/1/118 |
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