Comprehensive RNA-Seq-based study and metabolite profiling to identify genes involved in podophyllotoxin biosynthesis in Linum album Kotschy ex Boiss. (Linaceae)
Abstract Linum album is a well-known rich source of anticancer compounds, i.e., podophyllotoxin (PTOX) and other lignans. These compounds play an important role in the plant’s defensive system. The RNA-Seq data of flax (L. usitatissimum) were analyzed under various biotic and abiotic stresses to com...
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| Format: | Article |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-36102-7 |
| _version_ | 1797806758427623424 |
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| author | Zahra Danaeipour Ghasemali Garoosi Masoud Tohidfar Mohammad Reza Bakhtiarizadeh Mohammad Hossein Mirjalili |
| author_facet | Zahra Danaeipour Ghasemali Garoosi Masoud Tohidfar Mohammad Reza Bakhtiarizadeh Mohammad Hossein Mirjalili |
| author_sort | Zahra Danaeipour |
| collection | DOAJ |
| description | Abstract Linum album is a well-known rich source of anticancer compounds, i.e., podophyllotoxin (PTOX) and other lignans. These compounds play an important role in the plant’s defensive system. The RNA-Seq data of flax (L. usitatissimum) were analyzed under various biotic and abiotic stresses to comprehend better the importance of lignans in plant defense responses. Then, the association between the lignan contents and some related gene expressions was experimented with HPLC and qRT-PCR, respectively. Transcriptomic profiling showed a specific expression pattern in different organs, and just the commonly regulated gene EP3 was detected with a significant increase under all stresses. The in silico analysis of the PTOX biosynthesis pathway identified a list of genes, including laccase (LAC11), lactoperoxidase (POD), 4-coumarate-CoA ligase (4CL), and secoisolariciresinol dehydrogenase (SDH). These genes increased significantly under individual stresses. The HPLC analysis showed that the measured lignan contents generally increased under stress. In contrast, a quantitative expression of the genes involved in this pathway using qRT-PCR showed a different pattern that seems to contribute to regulating PTOX content in response to stress. Identified modifications of critical genes related to PTOX biosynthesis in response to multiple stresses can provide a baseline for improving PTOX content in L. album. |
| first_indexed | 2024-03-13T06:12:13Z |
| format | Article |
| id | doaj.art-1ace009149ea4675abf243a3c6582c07 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T06:12:13Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1ace009149ea4675abf243a3c6582c072023-06-11T11:11:07ZengNature PortfolioScientific Reports2045-23222023-06-0113111510.1038/s41598-023-36102-7Comprehensive RNA-Seq-based study and metabolite profiling to identify genes involved in podophyllotoxin biosynthesis in Linum album Kotschy ex Boiss. (Linaceae)Zahra Danaeipour0Ghasemali Garoosi1Masoud Tohidfar2Mohammad Reza Bakhtiarizadeh3Mohammad Hossein Mirjalili4Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International UniversityDepartment of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International UniversityDepartment of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti UniversityDepartment of Animal and Poultry Science, College of Aburaihan, University of TehranDepartment of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti UniversityAbstract Linum album is a well-known rich source of anticancer compounds, i.e., podophyllotoxin (PTOX) and other lignans. These compounds play an important role in the plant’s defensive system. The RNA-Seq data of flax (L. usitatissimum) were analyzed under various biotic and abiotic stresses to comprehend better the importance of lignans in plant defense responses. Then, the association between the lignan contents and some related gene expressions was experimented with HPLC and qRT-PCR, respectively. Transcriptomic profiling showed a specific expression pattern in different organs, and just the commonly regulated gene EP3 was detected with a significant increase under all stresses. The in silico analysis of the PTOX biosynthesis pathway identified a list of genes, including laccase (LAC11), lactoperoxidase (POD), 4-coumarate-CoA ligase (4CL), and secoisolariciresinol dehydrogenase (SDH). These genes increased significantly under individual stresses. The HPLC analysis showed that the measured lignan contents generally increased under stress. In contrast, a quantitative expression of the genes involved in this pathway using qRT-PCR showed a different pattern that seems to contribute to regulating PTOX content in response to stress. Identified modifications of critical genes related to PTOX biosynthesis in response to multiple stresses can provide a baseline for improving PTOX content in L. album.https://doi.org/10.1038/s41598-023-36102-7 |
| spellingShingle | Zahra Danaeipour Ghasemali Garoosi Masoud Tohidfar Mohammad Reza Bakhtiarizadeh Mohammad Hossein Mirjalili Comprehensive RNA-Seq-based study and metabolite profiling to identify genes involved in podophyllotoxin biosynthesis in Linum album Kotschy ex Boiss. (Linaceae) Scientific Reports |
| title | Comprehensive RNA-Seq-based study and metabolite profiling to identify genes involved in podophyllotoxin biosynthesis in Linum album Kotschy ex Boiss. (Linaceae) |
| title_full | Comprehensive RNA-Seq-based study and metabolite profiling to identify genes involved in podophyllotoxin biosynthesis in Linum album Kotschy ex Boiss. (Linaceae) |
| title_fullStr | Comprehensive RNA-Seq-based study and metabolite profiling to identify genes involved in podophyllotoxin biosynthesis in Linum album Kotschy ex Boiss. (Linaceae) |
| title_full_unstemmed | Comprehensive RNA-Seq-based study and metabolite profiling to identify genes involved in podophyllotoxin biosynthesis in Linum album Kotschy ex Boiss. (Linaceae) |
| title_short | Comprehensive RNA-Seq-based study and metabolite profiling to identify genes involved in podophyllotoxin biosynthesis in Linum album Kotschy ex Boiss. (Linaceae) |
| title_sort | comprehensive rna seq based study and metabolite profiling to identify genes involved in podophyllotoxin biosynthesis in linum album kotschy ex boiss linaceae |
| url | https://doi.org/10.1038/s41598-023-36102-7 |
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