Polymorphism and molecular characteristics of the <i>CSN1S2</i> gene in river and swamp buffalo
<p>The <span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-casein (<span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-CN) is a member of the casein fam...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2020-09-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/63/345/2020/aab-63-345-2020.pdf |
| _version_ | 1818412933679415296 |
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| author | X. Fan S. Gao L. Fu L. Qiu Y. Miao |
| author_facet | X. Fan S. Gao L. Fu L. Qiu Y. Miao |
| author_sort | X. Fan |
| collection | DOAJ |
| description | <p>The <span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-casein (<span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-CN) is a member of the casein
family associated with milk traits in ruminants, but so far the buffalo
<i>CSN1S2</i> gene has not been well understood. In this work, the polymorphisms of
<i>CSN1S2</i> in river and swamp buffalo were detected using direct sequencing of polymerase chain reaction (PCR) products. As a result, 13 single nucleotide polymorphisms (SNPs) were identified in the coding sequence (CDS) of <i>CSN1S2</i> in two types
of buffalo, of which eight SNPs were non-synonymous. The amino acid changes
caused by c.580T<span class="inline-formula">></span>C and c.642C<span class="inline-formula">></span>G may affect the
function of buffalo <span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-CN. A total of 11 <i>CSN1S2</i> CDS haplotypes were
defined, and accordingly 11 variants of buffalo <span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-CN were
inferred and named. The <i>CSN1S2</i> CDSs of both types of buffalo were 669 nucleotides,
which encoded a precursor of 222 amino acids (AAs), and the first 15 AAs
constitute a signal peptide. The composition and physicochemical
characteristics of two types of buffalo <span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-CNs were similar
but slightly different from those of cattle <span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-CN. The
<span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-CN mature peptides of buffalo and the species of <i>Bos</i> genus contained a casein
domain, and their secondary structures were highly consistent, indicating
that they are functionally similar. The results here provide initial
insights into the variation, characteristics and biological function of
buffalo <i>CSN1S2</i>.</p> |
| first_indexed | 2024-12-14T10:55:11Z |
| format | Article |
| id | doaj.art-1acedd3e9a084826bdc23b46e4b4da25 |
| institution | Directory Open Access Journal |
| issn | 0003-9438 2363-9822 |
| language | English |
| last_indexed | 2024-12-14T10:55:11Z |
| publishDate | 2020-09-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Archives Animal Breeding |
| spelling | doaj.art-1acedd3e9a084826bdc23b46e4b4da252022-12-21T23:04:57ZengCopernicus PublicationsArchives Animal Breeding0003-94382363-98222020-09-016334535410.5194/aab-63-345-2020Polymorphism and molecular characteristics of the <i>CSN1S2</i> gene in river and swamp buffaloX. Fan0S. Gao1L. Fu2L. Qiu3Y. Miao4Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, Yunnan, China<p>The <span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-casein (<span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-CN) is a member of the casein family associated with milk traits in ruminants, but so far the buffalo <i>CSN1S2</i> gene has not been well understood. In this work, the polymorphisms of <i>CSN1S2</i> in river and swamp buffalo were detected using direct sequencing of polymerase chain reaction (PCR) products. As a result, 13 single nucleotide polymorphisms (SNPs) were identified in the coding sequence (CDS) of <i>CSN1S2</i> in two types of buffalo, of which eight SNPs were non-synonymous. The amino acid changes caused by c.580T<span class="inline-formula">></span>C and c.642C<span class="inline-formula">></span>G may affect the function of buffalo <span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-CN. A total of 11 <i>CSN1S2</i> CDS haplotypes were defined, and accordingly 11 variants of buffalo <span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-CN were inferred and named. The <i>CSN1S2</i> CDSs of both types of buffalo were 669 nucleotides, which encoded a precursor of 222 amino acids (AAs), and the first 15 AAs constitute a signal peptide. The composition and physicochemical characteristics of two types of buffalo <span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-CNs were similar but slightly different from those of cattle <span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-CN. The <span class="inline-formula"><i>α</i><sub><i>S</i>2</sub></span>-CN mature peptides of buffalo and the species of <i>Bos</i> genus contained a casein domain, and their secondary structures were highly consistent, indicating that they are functionally similar. The results here provide initial insights into the variation, characteristics and biological function of buffalo <i>CSN1S2</i>.</p>https://aab.copernicus.org/articles/63/345/2020/aab-63-345-2020.pdf |
| spellingShingle | X. Fan S. Gao L. Fu L. Qiu Y. Miao Polymorphism and molecular characteristics of the <i>CSN1S2</i> gene in river and swamp buffalo Archives Animal Breeding |
| title | Polymorphism and molecular characteristics of the <i>CSN1S2</i> gene in river and swamp buffalo |
| title_full | Polymorphism and molecular characteristics of the <i>CSN1S2</i> gene in river and swamp buffalo |
| title_fullStr | Polymorphism and molecular characteristics of the <i>CSN1S2</i> gene in river and swamp buffalo |
| title_full_unstemmed | Polymorphism and molecular characteristics of the <i>CSN1S2</i> gene in river and swamp buffalo |
| title_short | Polymorphism and molecular characteristics of the <i>CSN1S2</i> gene in river and swamp buffalo |
| title_sort | polymorphism and molecular characteristics of the i csn1s2 i gene in river and swamp buffalo |
| url | https://aab.copernicus.org/articles/63/345/2020/aab-63-345-2020.pdf |
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