Genetic study for seed germination and shattering in Euphorbia lagascae in response to different seed treatments
Euphorbia lagascae Spreng is a promising emerging oilseed crop, with its seed oil accounting for approximately 50% of the seed weight. Euphorbia oil contains a significant amount of vernolic acid, comprising two-thirds of its composition, which boasts various industrial applications, including actin...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024040064 |
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| author | Maram Istaitieh Mohsen Yoosefzadeh Najafabadi A. Michelle Edwards Jim Todd Rene Van Acker Istvan Rajcan |
| author_facet | Maram Istaitieh Mohsen Yoosefzadeh Najafabadi A. Michelle Edwards Jim Todd Rene Van Acker Istvan Rajcan |
| author_sort | Maram Istaitieh |
| collection | DOAJ |
| description | Euphorbia lagascae Spreng is a promising emerging oilseed crop, with its seed oil accounting for approximately 50% of the seed weight. Euphorbia oil contains a significant amount of vernolic acid, comprising two-thirds of its composition, which boasts various industrial applications, including acting as a stabilizer-plasticizer and natural dye. However, this species was known to have a high degree of seed-shattering and a low germination rate, which act as two important barriers to large-scale production and exploitation. Therefore, the objective of this study is to determine the genetic control of seed germination and seed-shattering traits in order to develop a reliable pipeline that would be applicable for industries and breeders to select superior E. lagascae lines and design a robust breeding scheme in a short time at reduced labor costs. For this objective, five different wild-type genotypes of E. lagascae that demonstrated high germination potential were crossed with an ethyl methanesulfonate (EMS) mutant genotype that produces non-shattering capsules. The F2 populations from two successful crosses (A and B) were separated into three different treated groups for seed germination evaluation and to study the segregation of 200 individuals per F2 population. The three treatments were: light, gibberellic acid (GA3), and control treatment. Consequently, plants treated with approximately 250 μmol/m2/s of light showed significant improvement in germination up to 75% in cross A and 82.4 % in cross B compared with the control plants and the group treated with 0.05% GA3. According to the chi-square test results, the inheritance pattern of seed germination in response to light treatment follows a 3:1 segregation ratio between germinated and non-germinated seeds, indicating a dominant gene action in the F2 generation. The same conclusion was followed for the shattering trait in the group treated with light, which was also simply inherited as a 3:1 ratio for shattering vs. non-shattering capsules. Our results emphasize the importance and significance of light treatment in producing uniform populations through acceptable germination and shattering resistance of the mutant genotypes of E. lagascae. This is the first report of light treatment that significantly improved seed germination of E. lagascae, which may enhance efforts in the development of this new industrial crop as a feedstock for vernolic acid production. |
| first_indexed | 2024-04-24T19:19:21Z |
| format | Article |
| id | doaj.art-1ad1a06d927a47389c6651c61ca093c3 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T19:19:21Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-1ad1a06d927a47389c6651c61ca093c32024-03-26T04:26:48ZengElsevierHeliyon2405-84402024-04-01107e27975Genetic study for seed germination and shattering in Euphorbia lagascae in response to different seed treatmentsMaram Istaitieh0Mohsen Yoosefzadeh Najafabadi1A. Michelle Edwards2Jim Todd3Rene Van Acker4Istvan Rajcan5Department of Plant Agriculture, University of Guelph, Guelph, ON, N1G 2W1, CanadaDepartment of Plant Agriculture, University of Guelph, Guelph, ON, N1G 2W1, CanadaOffice of Research, Ontario Agri-Food Innovation Alliance, University of Guelph, Guelph, ON, N1G 2W1, CanadaOntario Ministry of Agriculture, Food and Rural Affairs, 1283 Blueline Rd, Simcoe, ON, N3Y 4K3, CanadaDepartment of Plant Agriculture, University of Guelph, Guelph, ON, N1G 2W1, CanadaDepartment of Plant Agriculture, University of Guelph, Guelph, ON, N1G 2W1, Canada; Corresponding author.Euphorbia lagascae Spreng is a promising emerging oilseed crop, with its seed oil accounting for approximately 50% of the seed weight. Euphorbia oil contains a significant amount of vernolic acid, comprising two-thirds of its composition, which boasts various industrial applications, including acting as a stabilizer-plasticizer and natural dye. However, this species was known to have a high degree of seed-shattering and a low germination rate, which act as two important barriers to large-scale production and exploitation. Therefore, the objective of this study is to determine the genetic control of seed germination and seed-shattering traits in order to develop a reliable pipeline that would be applicable for industries and breeders to select superior E. lagascae lines and design a robust breeding scheme in a short time at reduced labor costs. For this objective, five different wild-type genotypes of E. lagascae that demonstrated high germination potential were crossed with an ethyl methanesulfonate (EMS) mutant genotype that produces non-shattering capsules. The F2 populations from two successful crosses (A and B) were separated into three different treated groups for seed germination evaluation and to study the segregation of 200 individuals per F2 population. The three treatments were: light, gibberellic acid (GA3), and control treatment. Consequently, plants treated with approximately 250 μmol/m2/s of light showed significant improvement in germination up to 75% in cross A and 82.4 % in cross B compared with the control plants and the group treated with 0.05% GA3. According to the chi-square test results, the inheritance pattern of seed germination in response to light treatment follows a 3:1 segregation ratio between germinated and non-germinated seeds, indicating a dominant gene action in the F2 generation. The same conclusion was followed for the shattering trait in the group treated with light, which was also simply inherited as a 3:1 ratio for shattering vs. non-shattering capsules. Our results emphasize the importance and significance of light treatment in producing uniform populations through acceptable germination and shattering resistance of the mutant genotypes of E. lagascae. This is the first report of light treatment that significantly improved seed germination of E. lagascae, which may enhance efforts in the development of this new industrial crop as a feedstock for vernolic acid production.http://www.sciencedirect.com/science/article/pii/S2405844024040064Euphorbia breedingGenetic controlGibberellic acidIndustrial cropVernolic acid |
| spellingShingle | Maram Istaitieh Mohsen Yoosefzadeh Najafabadi A. Michelle Edwards Jim Todd Rene Van Acker Istvan Rajcan Genetic study for seed germination and shattering in Euphorbia lagascae in response to different seed treatments Heliyon Euphorbia breeding Genetic control Gibberellic acid Industrial crop Vernolic acid |
| title | Genetic study for seed germination and shattering in Euphorbia lagascae in response to different seed treatments |
| title_full | Genetic study for seed germination and shattering in Euphorbia lagascae in response to different seed treatments |
| title_fullStr | Genetic study for seed germination and shattering in Euphorbia lagascae in response to different seed treatments |
| title_full_unstemmed | Genetic study for seed germination and shattering in Euphorbia lagascae in response to different seed treatments |
| title_short | Genetic study for seed germination and shattering in Euphorbia lagascae in response to different seed treatments |
| title_sort | genetic study for seed germination and shattering in euphorbia lagascae in response to different seed treatments |
| topic | Euphorbia breeding Genetic control Gibberellic acid Industrial crop Vernolic acid |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024040064 |
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