Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease
Trypanosoma cruzi strains from distinct geographic areas show differences in drug resistance and association between parasites genetic and treatment response has been observed. Considering that benznidazole (BZ) can reduce the parasite burden and tissues damage, even in not cured animals and individ...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2015-02-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000100086&lng=en&tlng=en |
| _version_ | 1797758486913744896 |
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| author | Jaquelline Carla Valamiel de Oliveira-Silva Girley Francisco Machado-de-Assis Maykon Tavares Oliveira Nívia Carolina Noguieira Paiva Márcio Sobreira Silva Araújo Cláudia Martins Carneiro Olindo Assis Martins-Filho Helen Rodrigues Martins Marta de Lana |
| author_facet | Jaquelline Carla Valamiel de Oliveira-Silva Girley Francisco Machado-de-Assis Maykon Tavares Oliveira Nívia Carolina Noguieira Paiva Márcio Sobreira Silva Araújo Cláudia Martins Carneiro Olindo Assis Martins-Filho Helen Rodrigues Martins Marta de Lana |
| author_sort | Jaquelline Carla Valamiel de Oliveira-Silva |
| collection | DOAJ |
| description | Trypanosoma cruzi strains from distinct geographic areas show differences in drug resistance and association between parasites genetic and treatment response has been observed. Considering that benznidazole (BZ) can reduce the parasite burden and tissues damage, even in not cured animals and individuals, the goal is to assess the drug response to BZ of T. cruzi II strains isolated from children of the Jequitinhonha Valley, state of Minas Gerais, Brazil, before treatment. Mice infected and treated with BZ in both phases of infection were compared with the untreated and evaluated by fresh blood examination, haemoculture, polymerase chain reaction, conventional (ELISA) and non-conventional (FC-ALTA) serologies. In mice treated in the acute phase, a significant decrease in parasitaemia was observed for all strains. Positive parasitological and/or serological tests in animals treated during the acute and chronic (95.1-100%) phases showed that most of the strains were BZ resistant. However, beneficial effect was demonstrated because significant reduction (p < 0.05%) and/or suppression of parasitaemia was observed in mice infected with all strains (acute phase), associated to reduction/elimination of inflammation and fibrosis for two/eight strains. BZ offered some benefit, even in not cured animals, what suggest that BZ use may be recommended at least for recent chronic infection of the studied region. |
| first_indexed | 2024-03-12T18:30:40Z |
| format | Article |
| id | doaj.art-1ad34a78cd964db8a2f0e951a646e454 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:30:40Z |
| publishDate | 2015-02-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-1ad34a78cd964db8a2f0e951a646e4542023-08-02T08:19:00ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602015-02-011101869410.1590/0074-02760140260S0074-02762015000100086Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas diseaseJaquelline Carla Valamiel de Oliveira-SilvaGirley Francisco Machado-de-AssisMaykon Tavares OliveiraNívia Carolina Noguieira PaivaMárcio Sobreira Silva AraújoCláudia Martins CarneiroOlindo Assis Martins-FilhoHelen Rodrigues MartinsMarta de LanaTrypanosoma cruzi strains from distinct geographic areas show differences in drug resistance and association between parasites genetic and treatment response has been observed. Considering that benznidazole (BZ) can reduce the parasite burden and tissues damage, even in not cured animals and individuals, the goal is to assess the drug response to BZ of T. cruzi II strains isolated from children of the Jequitinhonha Valley, state of Minas Gerais, Brazil, before treatment. Mice infected and treated with BZ in both phases of infection were compared with the untreated and evaluated by fresh blood examination, haemoculture, polymerase chain reaction, conventional (ELISA) and non-conventional (FC-ALTA) serologies. In mice treated in the acute phase, a significant decrease in parasitaemia was observed for all strains. Positive parasitological and/or serological tests in animals treated during the acute and chronic (95.1-100%) phases showed that most of the strains were BZ resistant. However, beneficial effect was demonstrated because significant reduction (p < 0.05%) and/or suppression of parasitaemia was observed in mice infected with all strains (acute phase), associated to reduction/elimination of inflammation and fibrosis for two/eight strains. BZ offered some benefit, even in not cured animals, what suggest that BZ use may be recommended at least for recent chronic infection of the studied region.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000100086&lng=en&tlng=enTrypanosoma cruzi IIbenznidazole responseacute and chronic phasesmurine modelJequitinhonha ValleyMGBrazil |
| spellingShingle | Jaquelline Carla Valamiel de Oliveira-Silva Girley Francisco Machado-de-Assis Maykon Tavares Oliveira Nívia Carolina Noguieira Paiva Márcio Sobreira Silva Araújo Cláudia Martins Carneiro Olindo Assis Martins-Filho Helen Rodrigues Martins Marta de Lana Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease Memorias do Instituto Oswaldo Cruz Trypanosoma cruzi II benznidazole response acute and chronic phases murine model Jequitinhonha Valley MG Brazil |
| title | Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease |
| title_full | Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease |
| title_fullStr | Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease |
| title_full_unstemmed | Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease |
| title_short | Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease |
| title_sort | experimental benznidazole treatment of trypanosoma cruzi ii strains isolated from children of the jequitinhonha valley minas gerais brazil with chagas disease |
| topic | Trypanosoma cruzi II benznidazole response acute and chronic phases murine model Jequitinhonha Valley MG Brazil |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000100086&lng=en&tlng=en |
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