Genetic analysis and gene mapping of a dwarf and liguleless mutation in barley
Leaf development underlies crop growth and productivity and has been a major target of crop domestication and improvement. However, most genes controlling leaf development in barley remain unknown. We identified a dwarf and liguleless (dl) mutant derived by ethylmethane sulfonate mutagenesis. The dl...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2022-08-01
|
| Series: | Crop Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514122000381 |
| _version_ | 1828374815601328128 |
|---|---|
| author | Baojian Guo Jiang Qi Dongfang Li Hongwei Sun Chao Lyu Feifei Wang Juan Zhu Ganggang Guo Rugen Xu |
| author_facet | Baojian Guo Jiang Qi Dongfang Li Hongwei Sun Chao Lyu Feifei Wang Juan Zhu Ganggang Guo Rugen Xu |
| author_sort | Baojian Guo |
| collection | DOAJ |
| description | Leaf development underlies crop growth and productivity and has been a major target of crop domestication and improvement. However, most genes controlling leaf development in barley remain unknown. We identified a dwarf and liguleless (dl) mutant derived by ethylmethane sulfonate mutagenesis. The dl mutant showed dramatic changes in shoot architecture compared with wild-type (Yangnongpi 5) plants. Besides lacking ligules, the dl mutant showed much shorter plant height (28 cm) than Yangnongpi 5 (78 cm). By map-based cloning, the dl gene was localized to a 56.58-kb genomic interval on the long arm of chromosome 7. A C-to-T single-nucleotide substitution was identified at exon position 790, and is a functional mutation resulting in a proline-to-serine substitution at the 264th amino acid residue of HORVU7Hr1G106960. Consequently, HORVU7Hr1G106960 was identified as the DL gene, encoding 269 amino acids and containing the Arabidopsis LSH1 and Oryza G1 (ALOG) domain. DL is highly similar to rice OsG1-LIKE 1/2 (OsG1L1/2) and sorghum AWN1/AWN1-10 at the amino acid level. Although the dl mutant allele showed no expression changes in selected tissues by real-time PCR, we propose HORVU7Hr1G106960 as a candidate gene conferring the dwarf and liguleless phenotype in barley. |
| first_indexed | 2024-04-14T07:37:20Z |
| format | Article |
| id | doaj.art-1ad3dc200fb0477aac99307f4a9eedc4 |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-04-14T07:37:20Z |
| publishDate | 2022-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-1ad3dc200fb0477aac99307f4a9eedc42022-12-22T02:05:37ZengKeAi Communications Co., Ltd.Crop Journal2214-51412022-08-0110410941102Genetic analysis and gene mapping of a dwarf and liguleless mutation in barleyBaojian Guo0Jiang Qi1Dongfang Li2Hongwei Sun3Chao Lyu4Feifei Wang5Juan Zhu6Ganggang Guo7Rugen Xu8Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology, Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, Jiangsu, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology, Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, Jiangsu, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology, Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, Jiangsu, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology, Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, Jiangsu, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology, Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, Jiangsu, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology, Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, Jiangsu, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology, Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, Jiangsu, ChinaKey Laboratory of Crop Germplasm Resources and Utilization (MOA), The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology, Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, Jiangsu, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, Jiangsu, China; Corresponding author.Leaf development underlies crop growth and productivity and has been a major target of crop domestication and improvement. However, most genes controlling leaf development in barley remain unknown. We identified a dwarf and liguleless (dl) mutant derived by ethylmethane sulfonate mutagenesis. The dl mutant showed dramatic changes in shoot architecture compared with wild-type (Yangnongpi 5) plants. Besides lacking ligules, the dl mutant showed much shorter plant height (28 cm) than Yangnongpi 5 (78 cm). By map-based cloning, the dl gene was localized to a 56.58-kb genomic interval on the long arm of chromosome 7. A C-to-T single-nucleotide substitution was identified at exon position 790, and is a functional mutation resulting in a proline-to-serine substitution at the 264th amino acid residue of HORVU7Hr1G106960. Consequently, HORVU7Hr1G106960 was identified as the DL gene, encoding 269 amino acids and containing the Arabidopsis LSH1 and Oryza G1 (ALOG) domain. DL is highly similar to rice OsG1-LIKE 1/2 (OsG1L1/2) and sorghum AWN1/AWN1-10 at the amino acid level. Although the dl mutant allele showed no expression changes in selected tissues by real-time PCR, we propose HORVU7Hr1G106960 as a candidate gene conferring the dwarf and liguleless phenotype in barley.http://www.sciencedirect.com/science/article/pii/S2214514122000381BarleyEMSLigulelessALOGShoot architecture |
| spellingShingle | Baojian Guo Jiang Qi Dongfang Li Hongwei Sun Chao Lyu Feifei Wang Juan Zhu Ganggang Guo Rugen Xu Genetic analysis and gene mapping of a dwarf and liguleless mutation in barley Crop Journal Barley EMS Liguleless ALOG Shoot architecture |
| title | Genetic analysis and gene mapping of a dwarf and liguleless mutation in barley |
| title_full | Genetic analysis and gene mapping of a dwarf and liguleless mutation in barley |
| title_fullStr | Genetic analysis and gene mapping of a dwarf and liguleless mutation in barley |
| title_full_unstemmed | Genetic analysis and gene mapping of a dwarf and liguleless mutation in barley |
| title_short | Genetic analysis and gene mapping of a dwarf and liguleless mutation in barley |
| title_sort | genetic analysis and gene mapping of a dwarf and liguleless mutation in barley |
| topic | Barley EMS Liguleless ALOG Shoot architecture |
| url | http://www.sciencedirect.com/science/article/pii/S2214514122000381 |
| work_keys_str_mv | AT baojianguo geneticanalysisandgenemappingofadwarfandligulelessmutationinbarley AT jiangqi geneticanalysisandgenemappingofadwarfandligulelessmutationinbarley AT dongfangli geneticanalysisandgenemappingofadwarfandligulelessmutationinbarley AT hongweisun geneticanalysisandgenemappingofadwarfandligulelessmutationinbarley AT chaolyu geneticanalysisandgenemappingofadwarfandligulelessmutationinbarley AT feifeiwang geneticanalysisandgenemappingofadwarfandligulelessmutationinbarley AT juanzhu geneticanalysisandgenemappingofadwarfandligulelessmutationinbarley AT ganggangguo geneticanalysisandgenemappingofadwarfandligulelessmutationinbarley AT rugenxu geneticanalysisandgenemappingofadwarfandligulelessmutationinbarley |