Histopathology of the Intervertebral Disc of <i>Nothobranchius furzeri</i>, a Fish Model of Accelerated Aging
Introduction: Osteoarthritis is a classical age-related disease, which affects millions of patients worldwide. To further understand the pathophysiology and to develop therapeutic strategies for this disease, animal models play a significant role. <i>Nothobranchius furzeri</i> is an esta...
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MDPI AG
2023-10-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/12/10/1305 |
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| author | Maria Butylina Katharina Wahl-Figlash Michael Kothmayer Katharina Gelles Oliver Pusch Peter Pietschmann |
| author_facet | Maria Butylina Katharina Wahl-Figlash Michael Kothmayer Katharina Gelles Oliver Pusch Peter Pietschmann |
| author_sort | Maria Butylina |
| collection | DOAJ |
| description | Introduction: Osteoarthritis is a classical age-related disease, which affects millions of patients worldwide. To further understand the pathophysiology and to develop therapeutic strategies for this disease, animal models play a significant role. <i>Nothobranchius furzeri</i> is an established model for accelerated aging that spontaneously develops spinal deformities. Although the bone properties of <i>N. furzeri</i> are well described, characteristics of the intervertebral discs are still unknown. The aim of this study was to investigate the characteristics of the intervertebral discs of healthy and deformed <i>N. furzeri</i>. Material and Methods: Intervertebral properties of healthy and deformed <i>N. furzeri</i> were investigated in 8-, 12-, 18- and 21.5-week-old male fish of the GRZ strain. For histological evaluations the fish were decalcified, paraffin-embedded and stained with (1) hematoxylin and eosin, (2) toluidine blue and (3) alcian blue/picrosirius red. Results: 8-week-old and deformed <i>N. furzeri</i> showed spongy-like tissue containing vacuolated notochord cells and a beginning formation of fibrous tissue in the central area. Older healthy fish showed fibrous tissue in the central region and a spongy-like tissue in the peripheral region. Conclusion: Our study revealed age- and disease-related alterations of the vertebral discs in <i>N. furzeri</i>. Further studies should investigate the utility of <i>N. furzeri</i> as a model for degenerative spine diseases. |
| first_indexed | 2024-03-10T21:25:11Z |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T21:25:11Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj.art-1ad522c0bc0f4f4687cd2aa6d6c838432023-11-19T15:43:25ZengMDPI AGBiology2079-77372023-10-011210130510.3390/biology12101305Histopathology of the Intervertebral Disc of <i>Nothobranchius furzeri</i>, a Fish Model of Accelerated AgingMaria Butylina0Katharina Wahl-Figlash1Michael Kothmayer2Katharina Gelles3Oliver Pusch4Peter Pietschmann5Institute for Pathophysiology and Allergy Research (IPA), Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaInstitute for Pathophysiology and Allergy Research (IPA), Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaCenter of Anatomy and Cell Biology, Medical University of Vienna, 1090 Vienna, AustriaInstitute for Pathophysiology and Allergy Research (IPA), Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaCenter of Anatomy and Cell Biology, Medical University of Vienna, 1090 Vienna, AustriaInstitute for Pathophysiology and Allergy Research (IPA), Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaIntroduction: Osteoarthritis is a classical age-related disease, which affects millions of patients worldwide. To further understand the pathophysiology and to develop therapeutic strategies for this disease, animal models play a significant role. <i>Nothobranchius furzeri</i> is an established model for accelerated aging that spontaneously develops spinal deformities. Although the bone properties of <i>N. furzeri</i> are well described, characteristics of the intervertebral discs are still unknown. The aim of this study was to investigate the characteristics of the intervertebral discs of healthy and deformed <i>N. furzeri</i>. Material and Methods: Intervertebral properties of healthy and deformed <i>N. furzeri</i> were investigated in 8-, 12-, 18- and 21.5-week-old male fish of the GRZ strain. For histological evaluations the fish were decalcified, paraffin-embedded and stained with (1) hematoxylin and eosin, (2) toluidine blue and (3) alcian blue/picrosirius red. Results: 8-week-old and deformed <i>N. furzeri</i> showed spongy-like tissue containing vacuolated notochord cells and a beginning formation of fibrous tissue in the central area. Older healthy fish showed fibrous tissue in the central region and a spongy-like tissue in the peripheral region. Conclusion: Our study revealed age- and disease-related alterations of the vertebral discs in <i>N. furzeri</i>. Further studies should investigate the utility of <i>N. furzeri</i> as a model for degenerative spine diseases.https://www.mdpi.com/2079-7737/12/10/1305<i>Nothobranchius furzeri</i>spinal deformityintervertebral discshistology |
| spellingShingle | Maria Butylina Katharina Wahl-Figlash Michael Kothmayer Katharina Gelles Oliver Pusch Peter Pietschmann Histopathology of the Intervertebral Disc of <i>Nothobranchius furzeri</i>, a Fish Model of Accelerated Aging Biology <i>Nothobranchius furzeri</i> spinal deformity intervertebral discs histology |
| title | Histopathology of the Intervertebral Disc of <i>Nothobranchius furzeri</i>, a Fish Model of Accelerated Aging |
| title_full | Histopathology of the Intervertebral Disc of <i>Nothobranchius furzeri</i>, a Fish Model of Accelerated Aging |
| title_fullStr | Histopathology of the Intervertebral Disc of <i>Nothobranchius furzeri</i>, a Fish Model of Accelerated Aging |
| title_full_unstemmed | Histopathology of the Intervertebral Disc of <i>Nothobranchius furzeri</i>, a Fish Model of Accelerated Aging |
| title_short | Histopathology of the Intervertebral Disc of <i>Nothobranchius furzeri</i>, a Fish Model of Accelerated Aging |
| title_sort | histopathology of the intervertebral disc of i nothobranchius furzeri i a fish model of accelerated aging |
| topic | <i>Nothobranchius furzeri</i> spinal deformity intervertebral discs histology |
| url | https://www.mdpi.com/2079-7737/12/10/1305 |
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