Production of Diamino propionic acid ammonia lyase by a new strain of <it>Salmonella typhimurium</it> PU011
<p>Abstract</p> <p>Background</p> <p>Seeds of the legume plant Lathyrus sativus, which is grown in arid and semi arid tropical regions, contain Diamino Propionic acid (DAP). DAP is a neurotoxin, which, when consumed, causes a disease called Lathyrism. Lathryrism may man...
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| Format: | Article |
| Language: | English |
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BMC
2002-03-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/2/5 |
| _version_ | 1818751409626021888 |
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| author | Shiva Kumar Vasanth V PremKumar PL Rupesh KR Jayachandran Seetharaman S |
| author_facet | Shiva Kumar Vasanth V PremKumar PL Rupesh KR Jayachandran Seetharaman S |
| author_sort | Shiva Kumar Vasanth V |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Seeds of the legume plant Lathyrus sativus, which is grown in arid and semi arid tropical regions, contain Diamino Propionic acid (DAP). DAP is a neurotoxin, which, when consumed, causes a disease called Lathyrism. Lathryrism may manifest as Neurolathyrism or Osteolathyrism, in which the nervous system, and bone formation respectively, are affected. DAP ammonia lyase is produced by a few microorganisms such as Salmonella typhi, Salmonella typhimurium and Pseudomonas, and is capable of detoxifying DAP.</p> <p>Results</p> <p>S. typhimurium PU011, a non-virulent bacterial strain isolated in our lab, was found to produce DAP ammonia lyase enzyme when grown in minimal medium containing DAP. There was a direct correlation between biomass yield and enzyme activity, until 16 h post inoculation in minimal medium containing DAP. Following ammonium sulphate precipitation and passing through Sephadex G100, CM-Sephadex and DEAE-Sephacel for crude enzyme extract preparation, about 68-fold enzyme purity was obtained. The purified enzyme gave maximum activity at pH 8.0 and was stable up to 45 degrees C. The Km value for the substrate was found to be 0.685mM, calculated from a Line Weaver Burk plot.</p> <p>Conclusion</p> <p>A new bacterial strain, S.typhimurium PU 011, which is capable of producing DAP ammonia lyase, was isolated.</p> |
| first_indexed | 2024-12-18T04:35:07Z |
| format | Article |
| id | doaj.art-1ad78baba373455698a36c51ecdc451b |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-18T04:35:07Z |
| publishDate | 2002-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-1ad78baba373455698a36c51ecdc451b2022-12-21T21:20:52ZengBMCBMC Microbiology1471-21802002-03-0121510.1186/1471-2180-2-5Production of Diamino propionic acid ammonia lyase by a new strain of <it>Salmonella typhimurium</it> PU011Shiva Kumar Vasanth VPremKumar PLRupesh KRJayachandran Seetharaman S<p>Abstract</p> <p>Background</p> <p>Seeds of the legume plant Lathyrus sativus, which is grown in arid and semi arid tropical regions, contain Diamino Propionic acid (DAP). DAP is a neurotoxin, which, when consumed, causes a disease called Lathyrism. Lathryrism may manifest as Neurolathyrism or Osteolathyrism, in which the nervous system, and bone formation respectively, are affected. DAP ammonia lyase is produced by a few microorganisms such as Salmonella typhi, Salmonella typhimurium and Pseudomonas, and is capable of detoxifying DAP.</p> <p>Results</p> <p>S. typhimurium PU011, a non-virulent bacterial strain isolated in our lab, was found to produce DAP ammonia lyase enzyme when grown in minimal medium containing DAP. There was a direct correlation between biomass yield and enzyme activity, until 16 h post inoculation in minimal medium containing DAP. Following ammonium sulphate precipitation and passing through Sephadex G100, CM-Sephadex and DEAE-Sephacel for crude enzyme extract preparation, about 68-fold enzyme purity was obtained. The purified enzyme gave maximum activity at pH 8.0 and was stable up to 45 degrees C. The Km value for the substrate was found to be 0.685mM, calculated from a Line Weaver Burk plot.</p> <p>Conclusion</p> <p>A new bacterial strain, S.typhimurium PU 011, which is capable of producing DAP ammonia lyase, was isolated.</p>http://www.biomedcentral.com/1471-2180/2/5 |
| spellingShingle | Shiva Kumar Vasanth V PremKumar PL Rupesh KR Jayachandran Seetharaman S Production of Diamino propionic acid ammonia lyase by a new strain of <it>Salmonella typhimurium</it> PU011 BMC Microbiology |
| title | Production of Diamino propionic acid ammonia lyase by a new strain of <it>Salmonella typhimurium</it> PU011 |
| title_full | Production of Diamino propionic acid ammonia lyase by a new strain of <it>Salmonella typhimurium</it> PU011 |
| title_fullStr | Production of Diamino propionic acid ammonia lyase by a new strain of <it>Salmonella typhimurium</it> PU011 |
| title_full_unstemmed | Production of Diamino propionic acid ammonia lyase by a new strain of <it>Salmonella typhimurium</it> PU011 |
| title_short | Production of Diamino propionic acid ammonia lyase by a new strain of <it>Salmonella typhimurium</it> PU011 |
| title_sort | production of diamino propionic acid ammonia lyase by a new strain of it salmonella typhimurium it pu011 |
| url | http://www.biomedcentral.com/1471-2180/2/5 |
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