Proteomic responses of two spring wheat cultivars to the combined water deficit and aphid (Metopolophium dirhodum) treatments
In the field, plants usually have to face the combined effects of abiotic and biotic stresses. In our study, two spring wheat cultivars—Septima and Quintus—were subjected to three water regimes [70%, 50%, and 40% soil water capacity (SWC)], aphid (Metopolophium dirhodum) infestation, or the combinat...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1005755/full |
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| author | Klára Kosová Pavel Vítámvás Jiří Skuhrovec Jan Vítámvás Jan Vítámvás Sébastien Planchon Jenny Renaut Pavel Saska |
| author_facet | Klára Kosová Pavel Vítámvás Jiří Skuhrovec Jan Vítámvás Jan Vítámvás Sébastien Planchon Jenny Renaut Pavel Saska |
| author_sort | Klára Kosová |
| collection | DOAJ |
| description | In the field, plants usually have to face the combined effects of abiotic and biotic stresses. In our study, two spring wheat cultivars—Septima and Quintus—were subjected to three water regimes [70%, 50%, and 40% soil water capacity (SWC)], aphid (Metopolophium dirhodum) infestation, or the combination of both stresses, i.e., water deficit (50%, 40% SWC) and aphids. The study has a 2 × 3 × 2 factorial design with three biological replicates. In the present study, the results of proteomic analysis using 2D-DIGE followed by MALDI-TOF/TOF protein identification are presented. Water deficit but also aphid infestation led to alterations in 113 protein spots including proteins assigned to a variety of biological processes ranging from signaling via energy metabolism, redox regulation, and stress and defense responses to secondary metabolism indicating a long-term adaptation to adverse conditions. The absence of specific proteins involved in plant response to herbivorous insects indicates a loss of resistance to aphids in modern wheat cultivars during the breeding process and is in accordance with the “plant vigor hypothesis.” Septima revealed enhanced tolerance with respect to Quintus as indicated by higher values of morphophysiological characteristics (fresh aboveground biomass, leaf length, osmotic potential per full water saturation) and relative abundance of proteins involved in mitochondrial respiration and ATP biosynthesis. |
| first_indexed | 2024-04-13T12:17:55Z |
| format | Article |
| id | doaj.art-1ad8e987f9824a1b87d70ffa97400829 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T12:17:55Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1ad8e987f9824a1b87d70ffa974008292022-12-22T02:47:18ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-11-011310.3389/fpls.2022.10057551005755Proteomic responses of two spring wheat cultivars to the combined water deficit and aphid (Metopolophium dirhodum) treatmentsKlára Kosová0Pavel Vítámvás1Jiří Skuhrovec2Jan Vítámvás3Jan Vítámvás4Sébastien Planchon5Jenny Renaut6Pavel Saska7Plant Stress Biology and Biotechnology Group, Department of Plant Genetics and Breeding, Crop Research Institute, Prague, CzechiaPlant Stress Biology and Biotechnology Group, Department of Plant Genetics and Breeding, Crop Research Institute, Prague, CzechiaFunctional Diversity Group, Department of Plant Protection, Crop Research Institute, Prague, CzechiaPlant Stress Biology and Biotechnology Group, Department of Plant Genetics and Breeding, Crop Research Institute, Prague, CzechiaFaculty of Forestry and Wood Science, Czech University of Life Sciences, Prague, CzechiaBiotechnologies and Environmental Analytics Platform (BEAP), Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), Esch-sur-Alzette, LuxembourgBiotechnologies and Environmental Analytics Platform (BEAP), Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), Esch-sur-Alzette, LuxembourgFunctional Diversity Group, Department of Plant Protection, Crop Research Institute, Prague, CzechiaIn the field, plants usually have to face the combined effects of abiotic and biotic stresses. In our study, two spring wheat cultivars—Septima and Quintus—were subjected to three water regimes [70%, 50%, and 40% soil water capacity (SWC)], aphid (Metopolophium dirhodum) infestation, or the combination of both stresses, i.e., water deficit (50%, 40% SWC) and aphids. The study has a 2 × 3 × 2 factorial design with three biological replicates. In the present study, the results of proteomic analysis using 2D-DIGE followed by MALDI-TOF/TOF protein identification are presented. Water deficit but also aphid infestation led to alterations in 113 protein spots including proteins assigned to a variety of biological processes ranging from signaling via energy metabolism, redox regulation, and stress and defense responses to secondary metabolism indicating a long-term adaptation to adverse conditions. The absence of specific proteins involved in plant response to herbivorous insects indicates a loss of resistance to aphids in modern wheat cultivars during the breeding process and is in accordance with the “plant vigor hypothesis.” Septima revealed enhanced tolerance with respect to Quintus as indicated by higher values of morphophysiological characteristics (fresh aboveground biomass, leaf length, osmotic potential per full water saturation) and relative abundance of proteins involved in mitochondrial respiration and ATP biosynthesis.https://www.frontiersin.org/articles/10.3389/fpls.2022.1005755/fullspring wheatwater deficitherbivorous insectscombined stressproteome2D-DIGE |
| spellingShingle | Klára Kosová Pavel Vítámvás Jiří Skuhrovec Jan Vítámvás Jan Vítámvás Sébastien Planchon Jenny Renaut Pavel Saska Proteomic responses of two spring wheat cultivars to the combined water deficit and aphid (Metopolophium dirhodum) treatments Frontiers in Plant Science spring wheat water deficit herbivorous insects combined stress proteome 2D-DIGE |
| title | Proteomic responses of two spring wheat cultivars to the combined water deficit and aphid (Metopolophium dirhodum) treatments |
| title_full | Proteomic responses of two spring wheat cultivars to the combined water deficit and aphid (Metopolophium dirhodum) treatments |
| title_fullStr | Proteomic responses of two spring wheat cultivars to the combined water deficit and aphid (Metopolophium dirhodum) treatments |
| title_full_unstemmed | Proteomic responses of two spring wheat cultivars to the combined water deficit and aphid (Metopolophium dirhodum) treatments |
| title_short | Proteomic responses of two spring wheat cultivars to the combined water deficit and aphid (Metopolophium dirhodum) treatments |
| title_sort | proteomic responses of two spring wheat cultivars to the combined water deficit and aphid metopolophium dirhodum treatments |
| topic | spring wheat water deficit herbivorous insects combined stress proteome 2D-DIGE |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1005755/full |
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