Effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo (Bubalus bubalis) semen
The present study was carried out to investigate the effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo semen. Ejaculates (48) with mass motility ≥3+ from 3 bulls (16 from each bull) were collected as per standard procedure. Each ejaculate was d...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2016-11-01
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| Series: | Indian Journal of Animal Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/62993 |
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| author | S A LONE J K PRASAD S K GHOSH NARENDRA KUMAR SHOWKAT A BHAT G K DAS |
| author_facet | S A LONE J K PRASAD S K GHOSH NARENDRA KUMAR SHOWKAT A BHAT G K DAS |
| author_sort | S A LONE |
| collection | DOAJ |
| description |
The present study was carried out to investigate the effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo semen. Ejaculates (48) with mass motility ≥3+ from 3 bulls (16 from each bull) were collected as per standard procedure. Each ejaculate was divided into group I (control): diluted with Tris-egg yolk-glycerol dilutor up to 60×106 sperm/ml and group II: treated with cholesterol loaded cyclodextrin @ 3 mg/120×106 sperm and incubated at 37ºC for 10 min and then finally diluted with Tris-egg yolk-glycerol dilutor up to 60×106 sperm/ml. French medium straws (0.5 ml) were filled with the extended semen samples, sealed with polyvinyl alcohol powder and kept for 3 h at 5ºC for equilibration and then kept in automatic programmable freezer till temperature of straws reached –145°C followed by plunging into liquid nitrogen (– 196°C). Ejaculates were evaluated freshly, before freezing and after thawing for seminal attributes (progressive motility, viability, acrosomal integrity and hypo-osmotic swelling response) and antioxidants (superoxide dismutase, catalase, glutathione peroxidase and total antioxidants). After thawing, activity of superoxide dismutase, catalase and total antioxidants were significantly higher in group II as compared to group I. It is concluded that cholesterol loaded cyclodextrin enhances seminal antioxidant activity.
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| first_indexed | 2024-03-12T16:13:09Z |
| format | Article |
| id | doaj.art-1ada6e270ff64dd9810fa61733738423 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-03-12T16:13:09Z |
| publishDate | 2016-11-01 |
| publisher | Indian Council of Agricultural Research |
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| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-1ada6e270ff64dd9810fa617337384232023-08-09T09:44:12ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272016-11-01861110.56093/ijans.v86i11.62993Effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo (Bubalus bubalis) semenS A LONE0J K PRASAD1S K GHOSH2NARENDRA KUMAR3SHOWKAT A BHAT4G K DAS5ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-National Dairy Research Institute, Karnal, HaryanaICAR-National Dairy Research Institute, Karnal, HaryanaICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India The present study was carried out to investigate the effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo semen. Ejaculates (48) with mass motility ≥3+ from 3 bulls (16 from each bull) were collected as per standard procedure. Each ejaculate was divided into group I (control): diluted with Tris-egg yolk-glycerol dilutor up to 60×106 sperm/ml and group II: treated with cholesterol loaded cyclodextrin @ 3 mg/120×106 sperm and incubated at 37ºC for 10 min and then finally diluted with Tris-egg yolk-glycerol dilutor up to 60×106 sperm/ml. French medium straws (0.5 ml) were filled with the extended semen samples, sealed with polyvinyl alcohol powder and kept for 3 h at 5ºC for equilibration and then kept in automatic programmable freezer till temperature of straws reached –145°C followed by plunging into liquid nitrogen (– 196°C). Ejaculates were evaluated freshly, before freezing and after thawing for seminal attributes (progressive motility, viability, acrosomal integrity and hypo-osmotic swelling response) and antioxidants (superoxide dismutase, catalase, glutathione peroxidase and total antioxidants). After thawing, activity of superoxide dismutase, catalase and total antioxidants were significantly higher in group II as compared to group I. It is concluded that cholesterol loaded cyclodextrin enhances seminal antioxidant activity. https://epubs.icar.org.in/index.php/IJAnS/article/view/62993AntioxidantsBuffaloCholesterol loaded cyclodextrinCryopreservationSemen |
| spellingShingle | S A LONE J K PRASAD S K GHOSH NARENDRA KUMAR SHOWKAT A BHAT G K DAS Effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo (Bubalus bubalis) semen Indian Journal of Animal Sciences Antioxidants Buffalo Cholesterol loaded cyclodextrin Cryopreservation Semen |
| title | Effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo (Bubalus bubalis) semen |
| title_full | Effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo (Bubalus bubalis) semen |
| title_fullStr | Effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo (Bubalus bubalis) semen |
| title_full_unstemmed | Effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo (Bubalus bubalis) semen |
| title_short | Effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo (Bubalus bubalis) semen |
| title_sort | effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo bubalus bubalis semen |
| topic | Antioxidants Buffalo Cholesterol loaded cyclodextrin Cryopreservation Semen |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/62993 |
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