The prophylactic effect of probiotic Enterococcus lactis IW5 against different human cancer cells
Enterococcus lactis IW5 was obtained from human gut and the potential probiotic characteristics of this organism were then evaluated. Results showed that this strain was highly resistant to low pH and high bile salt and adhered strongly to Caco-2 human epithelial colorectal cell lines. The supernata...
| Hoofdauteurs: | , , , , |
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| Formaat: | Artikel |
| Taal: | English |
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Frontiers Media S.A.
2015-11-01
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| Reeks: | Frontiers in Microbiology |
| Onderwerpen: | |
| Online toegang: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01317/full |
| _version_ | 1830518031000797184 |
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| author | YOUSEF eNAMI Babak eHaghshenas Minoo eHaghshenas Norhafizah eAbdullah Ahmad eYari Khosroushahi |
| author_facet | YOUSEF eNAMI Babak eHaghshenas Minoo eHaghshenas Norhafizah eAbdullah Ahmad eYari Khosroushahi |
| author_sort | YOUSEF eNAMI |
| collection | DOAJ |
| description | Enterococcus lactis IW5 was obtained from human gut and the potential probiotic characteristics of this organism were then evaluated. Results showed that this strain was highly resistant to low pH and high bile salt and adhered strongly to Caco-2 human epithelial colorectal cell lines. The supernatant of E. lactis IW5 strongly inhibited the growth of several pathogenic bacteria and decreased the viability of different cancer cells, such as HeLa, AGS, HT-29, and MCF-7. Conversely, E. lactis IW5 did not inhibit the viability of normal FHs-74 cells. This strain did not generate toxic enzymes, including β-glucosidase, β-glucuronidase, and N-acetyl-β-glucosaminidase and was highly susceptible to ampicillin, gentamycin, penicillin, vancomycin, clindamycin, sulfamethoxazol, and chloramphenicol but resistant to erythromycin and tetracyclin. This study provided evidence for the effect of E. lactis IW5 on cancer cells. Therefore, E. lactis IW5, as a bioactive therapeutics, should be subjected to other relevant tests to verify the therapeutic suitability of this strain for clinical applications. |
| first_indexed | 2024-12-22T04:11:23Z |
| format | Article |
| id | doaj.art-1ae175047f7b4f6c814cd4c1c4cce7d0 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T04:11:23Z |
| publishDate | 2015-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1ae175047f7b4f6c814cd4c1c4cce7d02022-12-21T18:39:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-11-01610.3389/fmicb.2015.01317156531The prophylactic effect of probiotic Enterococcus lactis IW5 against different human cancer cellsYOUSEF eNAMI0Babak eHaghshenas1Minoo eHaghshenas2Norhafizah eAbdullah3Ahmad eYari Khosroushahi4Institute of Biosciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaInstitute of Biosciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaSchool of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of IranChemical and Environmental Engineering Department, Faculty of Engineering, University Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaDrug Applied Research Center, Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Islamic Republic of IranEnterococcus lactis IW5 was obtained from human gut and the potential probiotic characteristics of this organism were then evaluated. Results showed that this strain was highly resistant to low pH and high bile salt and adhered strongly to Caco-2 human epithelial colorectal cell lines. The supernatant of E. lactis IW5 strongly inhibited the growth of several pathogenic bacteria and decreased the viability of different cancer cells, such as HeLa, AGS, HT-29, and MCF-7. Conversely, E. lactis IW5 did not inhibit the viability of normal FHs-74 cells. This strain did not generate toxic enzymes, including β-glucosidase, β-glucuronidase, and N-acetyl-β-glucosaminidase and was highly susceptible to ampicillin, gentamycin, penicillin, vancomycin, clindamycin, sulfamethoxazol, and chloramphenicol but resistant to erythromycin and tetracyclin. This study provided evidence for the effect of E. lactis IW5 on cancer cells. Therefore, E. lactis IW5, as a bioactive therapeutics, should be subjected to other relevant tests to verify the therapeutic suitability of this strain for clinical applications.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01317/fullApoptosisCytotoxicityanticancerantibiotic susceptibilityenzyme activity |
| spellingShingle | YOUSEF eNAMI Babak eHaghshenas Minoo eHaghshenas Norhafizah eAbdullah Ahmad eYari Khosroushahi The prophylactic effect of probiotic Enterococcus lactis IW5 against different human cancer cells Frontiers in Microbiology Apoptosis Cytotoxicity anticancer antibiotic susceptibility enzyme activity |
| title | The prophylactic effect of probiotic Enterococcus lactis IW5 against different human cancer cells |
| title_full | The prophylactic effect of probiotic Enterococcus lactis IW5 against different human cancer cells |
| title_fullStr | The prophylactic effect of probiotic Enterococcus lactis IW5 against different human cancer cells |
| title_full_unstemmed | The prophylactic effect of probiotic Enterococcus lactis IW5 against different human cancer cells |
| title_short | The prophylactic effect of probiotic Enterococcus lactis IW5 against different human cancer cells |
| title_sort | prophylactic effect of probiotic enterococcus lactis iw5 against different human cancer cells |
| topic | Apoptosis Cytotoxicity anticancer antibiotic susceptibility enzyme activity |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01317/full |
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